Alignments for a candidate for astD in Rhodobacter viridis JA737

GapMind for catabolism of small carbon sources

Alignments for a candidate for astD in Rhodobacter viridis JA737

Align N-succinylglutamate 5-semialdehyde dehydrogenase 1; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase 1; SGSD 1 (uncharacterized)
to candidate WP_110805037.1 C8J30_RS07010 aldehyde dehydrogenase family protein

Query= curated2:Q12QD2
         (498 letters)



>NCBI__GCF_003217355.1:WP_110805037.1
          Length = 779

 Score =  213 bits (541), Expect = 3e-59
 Identities = 148/445 (33%), Positives = 229/445 (51%), Gaps = 23/445 (5%)

Query: 21  LAGKGPSFSSVNPANGEVIWQGLGADAGQVDAAITSARAAFYTWSAMSLTERLVIVEAFA 80
           +   GP F++ NPA GE + +   A A  V AA+ +ARAA   W+A+S  ER   + A A
Sbjct: 41  MTAPGPGFATQNPATGETLAEATQATAADVAAAVAAARAAAPGWAALSGHERAKWLYAIA 100

Query: 81  EQLKEHAELFARTIALETGKALWESRTEVGAMTGKIAISIKANAERTGTVE---NPMPGA 137
             L++    F     L+TGK + E+R         + ++I+      G  E   +  PG 
Sbjct: 101 RNLQKREAFFRVLEVLDTGKPIRETRDI------DLPLAIRHFYHHAGWAELSTDDFPG- 153

Query: 138 KAFIRHKPHGVVAVFGPYNFPGHLPNGHIVPALIAGNTVLFKPSELTPKVAELTMQLWQQ 197
                H P G V    P+NFP  +    + PAL AGNTV+ KP+E TP  A    ++  +
Sbjct: 154 -----HAPLGTVGAIIPWNFPLLMLAWKVAPALAAGNTVVLKPAEETPLTALAFAEMCLE 208

Query: 198 AGLPNGVLNLLQGEIATGKALASHKGIDGLFFTGSSNTGHLLHQQYAGQPGKILALEMGG 257
            GLP GVLN++ G+  TG ALA+   +D + FTGS+  G  L Q  AG  GK L LE+GG
Sbjct: 209 LGLPPGVLNIVTGDGTTGAALAT-SAVDKIAFTGSTEVGRSLRQATAGS-GKALTLELGG 266

Query: 258 NNPLIITEVANVDAAVHDIIQSAFISSGQRCTCARRLFIPKTANGDAILAKLLTSTAKIA 317
            +P ++   A++DAA+  ++ + + + GQ C+   R+F+ +T   +    KL+    K+ 
Sbjct: 267 KSPFLVFADADLDAAIEGVVAAVWTNQGQICSAGSRIFVAETV-AERFTDKLIARFDKLR 325

Query: 318 LGDPFAETQPFFGAMISDKAAAGMVKAQADIQAAGGVSLIELTQVTPGLG-FVTPG-IID 375
           LGDP  +     GA+IS  A    +++      A G  L+  +   P  G FV PG +++
Sbjct: 326 LGDPL-DKGTDIGAVIS-PAQRDRIQSLVARGVAAGARLVHSSAPLPAQGSFVAPGLLLE 383

Query: 376 VTDASPLADEEHFGPLLKVYRYTDFDAAIDEANNTSFGLSAGLLADSETDYQHFYRRIRA 435
               +P   EE FGP+  +  +     A++ AN + +GL+A + +++ T  Q    ++ A
Sbjct: 384 AQPTNPCMIEEIFGPIATLATFRTPSEAVELANASRYGLAASIWSENVTLAQDVAAKLVA 443

Query: 436 GIVNWNKPITGASSAAPFGGIGASG 460
           G V W    T   +AAPFGG+  SG
Sbjct: 444 GTV-WINSHTLFDAAAPFGGMRESG 467



 Score = 53.1 bits (126), Expect = 3e-11
 Identities = 51/171 (29%), Positives = 77/171 (45%), Gaps = 8/171 (4%)

Query: 64  WSAMSLTERLVIVEAFAEQLKEHAELFARTIALETGKALWESRTEVGAMTGKIAISIKAN 123
           WS M+   R  ++   AE L   A  F   +A ETG        EV A T ++A    A 
Sbjct: 565 WSKMTGHARAQVLYFLAENLSLRAAEFEARLA-ETG----HDPAEV-AQTIRLAFRAAAL 618

Query: 124 AER-TGTVENPMPGAKAFIRHKPHGVVAVFGPYNFPGHLPNGHIVPALIAGNTVLFKPSE 182
           A++  G V    P      R +P GV+ +      P       ++PA+ AGN V+  P+ 
Sbjct: 619 ADKIAGEVIQTKPQMLTLTRPEPWGVLGIACANRQPLLGLAALVLPAIAAGNRVVAIPAM 678

Query: 183 LTPKVAELTMQLWQQAGLPNGVLNLLQG-EIATGKALASHKGIDGLFFTGS 232
           + P VA    Q+   + +P GV+NL+ G      K LA H  +  L++ G+
Sbjct: 679 VQPLVAADFAQVLDTSDVPGGVVNLICGPRDDLAKVLAQHDDVAALWYAGT 729


Lambda     K      H
   0.317    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 972
Number of extensions: 47
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 498
Length of database: 779
Length adjustment: 37
Effective length of query: 461
Effective length of database: 742
Effective search space:   342062
Effective search space used:   342062
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources