Alignments for a candidate for BPHYT_RS16930 in Rhodobacter viridis JA737

GapMind for catabolism of small carbon sources

Alignments for a candidate for BPHYT_RS16930 in Rhodobacter viridis JA737

Align Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale)
to candidate WP_110805879.1 C8J30_RS10885 sugar ABC transporter ATP-binding protein

Query= uniprot:B2SYR5
         (512 letters)



>NCBI__GCF_003217355.1:WP_110805879.1
          Length = 511

 Score =  357 bits (917), Expect = e-103
 Identities = 207/500 (41%), Positives = 300/500 (60%), Gaps = 20/500 (4%)

Query: 12  KVFPGVRALDGVSFDVNVGQVHGLMGENGAGKSTLLKILGGEYQPDSGRVMIDGNEVRFT 71
           KV+PG RAL GV FD+ +G V+ L+GENGAGKSTL+K++ G      G + +DG E+RF 
Sbjct: 16  KVYPGTRALKGVDFDLRMGAVNVLVGENGAGKSTLMKLIAGVEDMTEGTITMDGREMRFR 75

Query: 72  SAASSIAAGIAVIHQELQYVPDLTVAENLLLGQLPNSLGWVN-----KREAKRFVRERLE 126
           + A ++AAGI ++ QEL   P+L+VAEN+ +G    + G ++      REA R + ERLE
Sbjct: 76  TKADAVAAGIGIVFQELNLFPNLSVAENIFIGH-ETTRGGIDIDIEAHREATRQLMERLE 134

Query: 127 AMGVALDPNAKLRKLSIAQRQMVEICKALLRNARVIALDEPTSSLSHRETEVLFKLVRDL 186
                + P+  L  L I Q+Q+VEI KAL +NAR++ LDEPTS+LS  E EVLF+++ +L
Sbjct: 135 Q---NIHPDTPLGNLRIGQQQIVEIAKALAQNARILILDEPTSALSAAEVEVLFRVIDEL 191

Query: 187 RADNRAMIYISHRMDEIYELCDACTIFRDGRKIASHPTLEGVTRDTIVSEMVGREISDIY 246
            A    ++YISHR++E+  + D  T+ RDG  I    ++EGV    IV  M+G    +  
Sbjct: 192 TAQGVGIVYISHRLEELIRVGDYITVLRDG-VITGARSMEGVDIPWIVKAMIGSSSKEYG 250

Query: 247 NYSARPLGEVRFAAKGIE-----GHALAQPASFEVRRGEIVGFFGLVGAGRSELMHLVYG 301
                  G   F A+ I      G       S  +R GEIVG +GL+GAGRSE +  V  
Sbjct: 251 RSEVANFGPEIFRAEDITLPRAGGGFTVDHVSLSIRSGEIVGLYGLMGAGRSEFLECVMA 310

Query: 302 ADHKKGGELLLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINISCRRHYL 361
                GG+  ++GKP+  R     I  GI L PEDRK +G++ + ++ EN+ +S    + 
Sbjct: 311 QHPHSGGKFWVEGKPLTERDVPGRIARGIALIPEDRKRDGLIQIMSIRENLTLSSLPSFT 370

Query: 362 RVGMFLDRKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLKVVIL 421
           ++   LD KKEA+TA  FIK L IK  S    +  LSGGNQQK ++ + L     KV+++
Sbjct: 371 KL-FHLDLKKEAKTAVEFIKRLTIKVASPENPVSSLSGGNQQKVVIGKALMTGP-KVLLM 428

Query: 422 DEPTRGIDVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGRISGELTR 481
           DEP+RGID+GAK E++  + +LA  G  I+ ++S+L EVL +SDRI+VM QGR++GE   
Sbjct: 429 DEPSRGIDIGAKAEVFRTMRRLAAEGLGILFVTSDLDEVLALSDRIIVMAQGRVTGEF-- 486

Query: 482 KDATEQS-VLSLALPQSSTA 500
              TE + V+S   P +  +
Sbjct: 487 PSGTEAAKVISATTPNAQNS 506



 Score = 85.9 bits (211), Expect = 3e-21
 Identities = 65/229 (28%), Positives = 113/229 (49%), Gaps = 12/229 (5%)

Query: 252 PLG-EVRFAAKGIEGHALAQPASFEVRRGEIVGFFGLVGAGRSELMHLVYGADHKKGGEL 310
           P+G  +R   K   G    +   F++R G +    G  GAG+S LM L+ G +    G +
Sbjct: 6   PIGLSIRGGVKVYPGTRALKGVDFDLRMGAVNVLVGENGAGKSTLMKLIAGVEDMTEGTI 65

Query: 311 LLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINI--SCRRHYLRVGMFLD 368
            +DG+ ++ R+  +A+  GI +     +E  +    +V+ENI I     R  + + +   
Sbjct: 66  TMDGREMRFRTKADAVAAGIGIV---FQELNLFPNLSVAENIFIGHETTRGGIDIDIEAH 122

Query: 369 RKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLKVVILDEPTRGI 428
           R+   +  +R  + +   TP    +I     G QQ   +++ LA+ + +++ILDEPT  +
Sbjct: 123 REATRQLMERLEQNIHPDTPLGNLRI-----GQQQIVEIAKALAQ-NARILILDEPTSAL 176

Query: 429 DVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGRISG 477
                  ++ VI +L  +G  IV IS  L E++ V D I V+R G I+G
Sbjct: 177 SAAEVEVLFRVIDELTAQGVGIVYISHRLEELIRVGDYITVLRDGVITG 225


Lambda     K      H
   0.320    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 624
Number of extensions: 29
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 511
Length adjustment: 35
Effective length of query: 477
Effective length of database: 476
Effective search space:   227052
Effective search space used:   227052
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources