Align ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized)
to candidate WP_110804034.1 C8J30_RS01980 carbohydrate ABC transporter permease
Query= reanno::pseudo13_GW456_L13:PfGW456L13_1896 (281 letters) >NCBI__GCF_003217355.1:WP_110804034.1 Length = 382 Score = 129 bits (323), Expect = 1e-34 Identities = 80/242 (33%), Positives = 124/242 (51%), Gaps = 14/242 (5%) Query: 42 SPEDIRTGNLLSWPTVIDGIGWIKAWDVVGGYFWNSVKITVPAVLISTFIGAMNGYVLSM 101 +P + TGN + VI G +G F N++ +T+PA +I I A Y L+ Sbjct: 152 APPRLTTGN---YARVITAEG-------IGRAFLNTMTVTIPATVIPILIAAFAAYALAW 201 Query: 102 WRFRGSQLFFGLLLFGCFLPFQTVLLPASFTLGKFGLANTTTGLVLVHVVYGLAFTTLFF 161 RF G L ++ +P Q L+P + GL + G+ L H +GL Sbjct: 202 MRFPGRALLVATIVGLLVVPLQLALIPLLKLHNQIGLNQSYLGIWLAHTGFGLPLAIYLL 261 Query: 162 RNYYVSIPDALVKAARLDGAGFFTIFLKILLPMSIPIVMVCLIWQFTQIWNDFLFGVVFA 221 RNY V +P ++++AR+DGA F IF +I+LP+S P + I+QF +WND L VF Sbjct: 262 RNYMVGLPREIIESARVDGATEFQIFRRIVLPLSFPALASFAIFQFLWVWNDLLVATVFL 321 Query: 222 SG--DAQPITVALNNLVNTSTGAKEYNVDMAAAMIAGLPTLLVYIFAGKYFLRGLTSGAV 279 D Q +T AL L+ + G ++ + A+A ++ L+V+ KY +RGL +G+V Sbjct: 322 GNATDTQVMTGALRALLGSRGG--DWEILAASAFVSIAVPLIVFFALQKYLVRGLLAGSV 379 Query: 280 KG 281 KG Sbjct: 380 KG 381 Lambda K H 0.329 0.143 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 291 Number of extensions: 16 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 281 Length of database: 382 Length adjustment: 28 Effective length of query: 253 Effective length of database: 354 Effective search space: 89562 Effective search space used: 89562 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory