GapMind for catabolism of small carbon sources

 

L-histidine catabolism in Rhodobacter viridis JA737

Best path

hisP, hisM, hisQ, hisJ, hutH, hutU, hutI, hutG

Rules

Overview: Histidine utilization in GapMind is based on MetaCyc pathways L-histidine degradation I (link) or II (link). These pathways are very similar. Other pathways in MetaCyc (III-VI) are not complete or are not reported in prokaryotes, so they are not included.

48 steps (30 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
hisP L-histidine ABC transporter, ATPase component HisP C8J30_RS16150 C8J30_RS12895
hisM L-histidine ABC transporter, permease component 1 (HisM) C8J30_RS16145 C8J30_RS12910
hisQ L-histidine ABC transporter, permease component 2 (HisQ) C8J30_RS16140 C8J30_RS06530
hisJ L-histidine ABC transporter, substrate-binding component HisJ C8J30_RS16135 C8J30_RS12900
hutH histidine ammonia-lyase
hutU urocanase C8J30_RS09465
hutI imidazole-5-propionate hydrolase
hutG N-formiminoglutamate formiminohydrolase C8J30_RS02875
Alternative steps:
aapJ L-histidine ABC transporter, substrate-binding component AapJ C8J30_RS00060 C8J30_RS05885
aapM L-histidine ABC transporter, permease component 2 (AapM) C8J30_RS05895 C8J30_RS00070
aapP L-histidine ABC transporter, ATPase component AapP C8J30_RS05900 C8J30_RS16150
aapQ L-histidine ABC transporter, permease component 1 (AapQ) C8J30_RS05890 C8J30_RS00065
Ac3H11_2554 ABC transporter for L-Histidine, permease component 2 C8J30_RS16140 C8J30_RS16145
Ac3H11_2555 L-histidine ABC transporter, substrate-binding component 2 C8J30_RS16135 C8J30_RS12900
Ac3H11_2560 L-histidine ABC transporter, ATPase component C8J30_RS08980 C8J30_RS00305
Ac3H11_2561 L-histidine ABC transporter, permease component 1 C8J30_RS00300 C8J30_RS01665
Ac3H11_2562 L-histidine ABC transporter, substrate-binding component 1
bgtA L-histidine ABC transporter, ATPase component BgtA C8J30_RS05900 C8J30_RS16150
bgtB L-histidine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
BPHYT_RS24000 L-histidine ABC transporter, substrate-binding component C8J30_RS16135
BPHYT_RS24005 L-histidine ABC transporter, permease component 1 C8J30_RS16140 C8J30_RS06530
BPHYT_RS24010 L-histidine ABC transporter, permease component 2 C8J30_RS16145 C8J30_RS12910
BPHYT_RS24015 L-histidine ABC transporter, ATPase component C8J30_RS16150 C8J30_RS12895
braC ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC
braD ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD) C8J30_RS08775
braE ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE) C8J30_RS05180
braF ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF) C8J30_RS05195 C8J30_RS08765
braG ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG) C8J30_RS08760 C8J30_RS05170
Ga0059261_1577 L-histidine transporter
hutF N-formiminoglutamate deiminase
hutG' N-formylglutamate amidohydrolase C8J30_RS05295
hutV L-histidine ABC transporter, ATPase component HutV C8J30_RS14465 C8J30_RS16975
hutW L-histidine ABC transporter, permease component HutW C8J30_RS14470 C8J30_RS16985
hutX L-histidine ABC transporter, substrate-binding component HutX
LAT2 L-histidine transporter
LHT L-histidine transporter
natA L-histidine ABC transporter, ATPase component 1 (NatA) C8J30_RS02060 C8J30_RS08765
natB L-histidine ABC transporter, substrate-binding component NatB
natC L-histidine ABC transporter, permease component 1 (NatC)
natD L-histidine ABC transporter, permease component 2 (NatD) C8J30_RS02045
natE L-histidine ABC transporter, ATPase component 2 (NatE) C8J30_RS02055 C8J30_RS08760
PA5503 L-histidine ABC transporter, ATPase component C8J30_RS16975 C8J30_RS16150
PA5504 L-histidine ABC transporter, permease component
PA5505 L-histidine ABC transporter, substrate-binding component
permease L-histidine permease
PTR2 L-histidine:H+ symporter
S15A3 L-histidine transporter
SLC38A3 L-histidine:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory