Alignments for a candidate for fadB in Rhodobacter viridis JA737

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadB in Rhodobacter viridis JA737

Align fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized)
to candidate WP_110804971.1 C8J30_RS06890 3-hydroxyacyl-CoA dehydrogenase

Query= CharProtDB::CH_003230
         (714 letters)



>NCBI__GCF_003217355.1:WP_110804971.1
          Length = 728

 Score =  395 bits (1014), Expect = e-114
 Identities = 261/724 (36%), Positives = 373/724 (51%), Gaps = 35/724 (4%)

Query: 7   FTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFI 66
           FT ++  D +AVIT DV  + MN +  E  +Q+ A++     +   +G+V  S K D F 
Sbjct: 4   FTCDLGADGVAVITWDVADKSMNVMSLEGFAQLSALVDGCLADPACKGIVLTSGKKD-FA 62

Query: 67  AGADINMIGNCKTAQEAEALARQGQQLMAEIH--------------ALPIQVIAAIHGAC 112
            G D+N+I   + A  AEA+     Q+ A +               A PI  +  + G  
Sbjct: 63  GGMDLNVIAKMR-AGGAEAIFAGVMQMHAVLRKIERAGMDPKTLKGAKPIACV--LPGTA 119

Query: 113 LGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGK 172
           LG GLEL L+CH     ++PK  +GLPE+ +G+ PG+GGT RL R +G   A   +L GK
Sbjct: 120 LGIGLELPLSCHRIFAAENPKAKIGLPEIMVGIFPGAGGTTRLVRKMGAMMAAPFLLEGK 179

Query: 173 QLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAG--PLGRALLFK 230
               K A   G++D+V    +       L   +    +P   +   + G  P   A    
Sbjct: 180 LSDPKAAKAAGIIDEVCADPMAAARDWVLKATDADLVKPWDAKGYKMPGGAPYHPAGFMT 239

Query: 231 MVGKKT--EHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQAL-RS 287
            VG       KT G YPA + +L  V  G           EAR F ++ M P S A+ RS
Sbjct: 240 YVGASAMVHGKTMGVYPAAKALLSAVYEGALVPFDQALRIEARWFTKVLMNPSSGAMIRS 299

Query: 288 IFFASTDVKKDPGSDAPPAPLNSV---GILGGGLMGGGIAYVTACKAGIPVRIKDINPQG 344
           +F     ++K  G++ P     SV   G+LG G+MG GIAYV A  AGI V + D + + 
Sbjct: 300 LFINKEALEK--GANRPKVADQSVTKLGVLGAGMMGAGIAYVAA-NAGIEVVLIDASQEA 356

Query: 345 INHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQ 404
            +    YS   L+  ++RR +  +++ + L  I+ TTDY   A  DL++EAVFE+ ++K 
Sbjct: 357 ADRGKSYSTGILDKGIQRRKVTEAKKAEVLDRITATTDYAALAGCDLVVEAVFEDPKVKA 416

Query: 405 QMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAG 464
           ++ A+ E    A  IFA+NTS+LPI  +A  + RP   IG+HFFSPV+KM LVEII    
Sbjct: 417 EVTAKAEAVIGAGAIFATNTSTLPISGLAKASQRPANFIGIHFFSPVDKMNLVEIIRGKD 476

Query: 465 TSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALV 524
           T    +A  +   +   KTPIVV D   FY NR + PYINE IRM+ +G     ++ A  
Sbjct: 477 TGEVAVAKALDFVRAIRKTPIVVNDARFFYANRCIIPYINEGIRMVAEGVNPVLVENAAK 536

Query: 525 KFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERF--SAPANVVSSILNDDRKGRKNGRG 582
             G P+GP+QL+DE  ID G KI    +AA G+ +   A   VV  +    R G+K   G
Sbjct: 537 LVGMPLGPLQLVDETSIDLGVKIAKATKAAMGDAYPDGAVDEVVFWMAEQGRLGKKADAG 596

Query: 583 FYLYGQKGRKSKKQVDPAIYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSV 642
           FY Y    R        A YP    Q         V  R +M+ + EAVR  ++ V+  +
Sbjct: 597 FYTYDAGKRGGMWDGLSAQYPRAADQP----DLTHVQHRLLMVQVLEAVRAFEQGVLTDI 652

Query: 643 RDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARG 702
           R+GD+GA+ G GF P+ GGPF ++D LGA   V I   LA QYG+RF P   L ++ A+G
Sbjct: 653 REGDVGAILGWGFAPWSGGPFAWLDILGAARAVEICDGLAAQYGARFEPPALLRDIAAKG 712

Query: 703 ESFW 706
           +SF+
Sbjct: 713 DSFY 716


Lambda     K      H
   0.319    0.137    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1065
Number of extensions: 51
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 714
Length of database: 728
Length adjustment: 40
Effective length of query: 674
Effective length of database: 688
Effective search space:   463712
Effective search space used:   463712
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources