GapMind for catabolism of small carbon sources

 

Protein WP_111392228.1 in Algoriphagus aquaeductus T4

Annotation: NCBI__GCF_003253485.1:WP_111392228.1

Length: 260 amino acids

Source: GCF_003253485.1 in NCBI

Candidate for 6 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
ethanol catabolism etoh-dh-nad lo alcohol dehydrogenase (EC 1.1.1.1) (characterized) 36% 96% 164.1 levodione reductase monomer 50% 260.4
xylitol catabolism xdhA lo D-xylulose reductase (EC 1.1.1.9) (characterized) 35% 98% 161.8 levodione reductase monomer 50% 260.4
D-xylose catabolism xdhA lo D-xylulose reductase (EC 1.1.1.9) (characterized) 35% 98% 161.8 levodione reductase monomer 50% 260.4
L-rhamnose catabolism LRA5 lo 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)); 2-keto-3-deoxy-L-rhamnonate dehydrogenase; KDRDH; L-KDR dehydrogenase; L-KDR 4-dehydrogenase; EC 1.1.1.401 (characterized) 34% 96% 139.4 levodione reductase monomer 50% 260.4
L-isoleucine catabolism ivdG lo 3-hydroxy-2-methylbutyryl-CoA dehydrogenase subunit (EC 1.1.1.178) (characterized) 35% 97% 132.1 levodione reductase monomer 50% 260.4
D-sorbitol (glucitol) catabolism srlD lo Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (characterized) 32% 97% 130.6 levodione reductase monomer 50% 260.4

Sequence Analysis Tools

View WP_111392228.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKNKVMIITGAAMGLGYAAAKELASKGAHLVLVDYNQKSLEDAKSEIIKEFPQAKIITVI
ADVSKEDAVKNYVDEAVKAFGRIDGFYNNAGIEGRQAPITEYDVDVFKKVIDINLMGVYY
GMRYVIPVMQKQQFGRIVNVASVGGIRGVLNQTPYVASKHAVSGMTKNAALEYGRDGILT
NAIAPGAILTPMVAEAFKQVNPADPKAAESEYAQRNPTKRLGKPEEVAKLVAFLLSEDNG
YVSGQTIAIDGGESNIYGNS

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory