GapMind for catabolism of small carbon sources

 

Protein WP_111392913.1 in Algoriphagus aquaeductus T4

Annotation: NCBI__GCF_003253485.1:WP_111392913.1

Length: 255 amino acids

Source: GCF_003253485.1 in NCBI

Candidate for 7 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
ethanol catabolism etoh-dh-nad lo alcohol dehydrogenase (EC 1.1.1.1) (characterized) 36% 96% 167.2 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2
D-galactose catabolism galdh lo Probable galactose dehydrogenase GalD; EC 1.1.1.- (characterized) 32% 98% 136.3 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2
lactose catabolism galdh lo Probable galactose dehydrogenase GalD; EC 1.1.1.- (characterized) 32% 98% 136.3 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2
L-arabinose catabolism xacB lo L-arabinose 1-dehydrogenase; D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized) 31% 92% 121.3 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2
L-fucose catabolism fdh lo Short-chain dehydrogenase (characterized, see rationale) 33% 97% 110.9 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2
2-deoxy-D-ribonate catabolism deoxyribonate-dehyd lo 2-deoxy-D-ribonate 3-dehydrogenase (characterized) 31% 94% 109 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2
2-deoxy-D-ribose catabolism deoxyribonate-dehyd lo 2-deoxy-D-ribonate 3-dehydrogenase (characterized) 31% 94% 109 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD; EC 1.1.1.184 42% 192.2

Sequence Analysis Tools

View WP_111392913.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MDLSSVFSLEGKVALITGASKGIGFSIAEIFAAAGAKVVISSRKQDALDGMAEKLRSKGY
VGSGIACNVGNMDELPALVEKTVALYGTIDILVNNAATNPVFGPVHETSLEAFDKIMNVN
VKAAFELCRLCYPHLRKSSGASVINISSIGGISPEHGLGIYSVSKAALISLTKVFAKEWG
DSKIRVNAICPGLIQTKFSEALWTNEKIMSMIMKQLAIKRAGTSEEIGAMALFLASSASS
YTTGGVFTADGGFTI

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory