Annotation: NCBI__GCF_003253485.1:WP_111394606.1
Length: 347 amino acids
Source: GCF_003253485.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| xylitol catabolism | xdhA | lo | Sorbitol dehydrogenase; SDH; EC 1.1.1.-; Glucitol dehydrogenase; L-iditol 2-dehydrogenase; EC 1.1.1.14; Polyol dehydrogenase; Xylitol dehydrogenase; EC 1.1.1.9 (uncharacterized) | 36% | 95% | 202.2 | D-arabitol-phosphate dehydrogenase; APDH; EC 1.1.1.301 | 36% | 213.0 |
| D-xylose catabolism | xdhA | lo | Sorbitol dehydrogenase; SDH; EC 1.1.1.-; Glucitol dehydrogenase; L-iditol 2-dehydrogenase; EC 1.1.1.14; Polyol dehydrogenase; Xylitol dehydrogenase; EC 1.1.1.9 (uncharacterized) | 36% | 95% | 202.2 | D-arabitol-phosphate dehydrogenase; APDH; EC 1.1.1.301 | 36% | 213.0 |
| xylitol catabolism | x5p-reductase | lo | Lmo2663 protein (characterized, see rationale) | 32% | 100% | 182.6 | D-arabitol-phosphate dehydrogenase; APDH; EC 1.1.1.301 | 36% | 213.0 |
| L-threonine catabolism | tdh | lo | L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 (uncharacterized) | 31% | 94% | 148.7 | D-arabitol-phosphate dehydrogenase; APDH; EC 1.1.1.301 | 36% | 213.0 |
View WP_111394606.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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MSSTKIPAVVNYSEQKHSVEIREIPVPEIGEEDILLEVENVGVCGSDLHQWTSDHSWPVN YPVVLGHEFGGGIAALGSRVSGWKVGDRVVSETAAVIDTQNPMSKVGLYNLDPTRKGFGY GVNGAMTRYVRVPARCLHTVPENLSFEEACLTEPCCVAFNSLVGNSHIKPGDRVIVIGPG TIGILCAAVARLCGAEVAVLGLEADRGRLEIAKNAYGCDPLIGEDAAEAWARKRDGLGAD LIVDAAGHSITLKTAMKLVRPNGQITKVGWGPQPLNFSIDPIVQKNVRLQGSFSHNWPIW ERVIALLASGALNVKPIIGGVWPITEWHTAFEKMHTGQIVKAVLKPV
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory