GapMind for catabolism of small carbon sources

 

L-leucine catabolism in Algoriphagus aquaeductus T4

Best path

leuT, ilvE, bkdA, bkdB, bkdC, lpd, liuA, liuB, liuD, liuC, liuE, atoA, atoD, atoB

Rules

Overview: Leucine degradation in GapMind is based on MetaCyc pathway L-leucine degradation I, via branched alpha-keto acid dehydrogenase (link). Other pathways for are not included here because they are not linked to sequence (link) or do not result in carbon incorporation.

39 steps (20 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
leuT L-leucine:Na+ symporter LeuT CLV31_RS10590
ilvE L-leucine transaminase CLV31_RS09355 CLV31_RS20475
bkdA branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit CLV31_RS20050 CLV31_RS15130
bkdB branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit CLV31_RS20050 CLV31_RS07115
bkdC branched-chain alpha-ketoacid dehydrogenase, E2 component CLV31_RS13860 CLV31_RS09795
lpd branched-chain alpha-ketoacid dehydrogenase, E3 component CLV31_RS09800 CLV31_RS07900
liuA isovaleryl-CoA dehydrogenase CLV31_RS09630 CLV31_RS14820
liuB 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit CLV31_RS08220 CLV31_RS02070
liuD 3-methylcrotonyl-CoA carboxylase, beta subunit CLV31_RS17930 CLV31_RS11745
liuC 3-methylglutaconyl-CoA hydratase CLV31_RS07705 CLV31_RS05805
liuE hydroxymethylglutaryl-CoA lyase CLV31_RS09190
atoA acetoacetyl-CoA transferase, A subunit CLV31_RS11365 CLV31_RS16400
atoD acetoacetyl-CoA transferase, B subunit CLV31_RS11360 CLV31_RS16400
atoB acetyl-CoA C-acetyltransferase CLV31_RS09120 CLV31_RS03395
Alternative steps:
aacS acetoacetyl-CoA synthetase CLV31_RS02320
AAP1 L-leucine permease AAP1
aapJ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), substrate-binding component AapJ
aapM ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM)
aapP ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP CLV31_RS07770 CLV31_RS15400
aapQ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ)
Bap2 L-leucine permease Bap2
bcaP L-leucine uptake transporter BcaP
brnQ L-leucine:Na+ symporter BrnQ/BraB
livF L-leucine ABC transporter, ATPase component 1 (LivF/BraG) CLV31_RS04135 CLV31_RS07165
livG L-leucine ABC transporter, ATPase component 2 (LivG/BraF) CLV31_RS04135 CLV31_RS07770
livH L-leucine ABC transporter, permease component 1 (LivH/BraD)
livJ L-leucine ABC transporter, substrate-binding component (LivJ/LivK/BraC/BraC3)
livM L-leucine ABC transporter, permease component 2 (LivM/BraE)
natA L-leucine ABC transporter, ATPase component 1 (NatA) CLV31_RS04135
natB L-leucine ABC transporter, substrate-binding component NatB
natC L-leucine ABC transporter, permease component 1 (NatC)
natD L-leucine ABC transporter, permease component 2 (NatD)
natE L-leucine ABC transporter, ATPase component 2 (NatE) CLV31_RS04135 CLV31_RS01215
ofo branched-chain alpha-ketoacid:ferredoxin oxidoreductase, fused
ofoA branched-chain alpha-ketoacid:ferredoxin oxidoreductase, alpha subunit OfoA
ofoB branched-chain alpha-ketoacid:ferredoxin oxidoreductase, beta subunit OfoB
vorA branched-chain alpha-ketoacid:ferredoxin oxidoreductase, alpha subunit VorA
vorB branched-chain alpha-ketoacid:ferredoxin oxidoreductase, beta subunit VorB
vorC branched-chain alpha-ketoacid:ferredoxin oxidoreductase, gamma subunit VorC

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory