Alignments for a candidate for xad in Marinomonas arctica 328

GapMind for catabolism of small carbon sources

Alignments for a candidate for xad in Marinomonas arctica 328

Align Xylonate dehydratase (EC 4.2.1.82) (characterized)
to candidate WP_111608298.1 DK187_RS15785 dihydroxy-acid dehydratase

Query= reanno::pseudo5_N2C3_1:AO356_28760
         (594 letters)



>NCBI__GCF_003259225.1:WP_111608298.1
          Length = 573

 Score =  367 bits (942), Expect = e-106
 Identities = 218/568 (38%), Positives = 319/568 (56%), Gaps = 25/568 (4%)

Query: 9   LRSEQWFNDPAHADMTAL-YVERYMNYGMTREELQSGRPIIGIAQTGSDLTPCNRHHLEL 67
           LRS++WF   A  +M A  + +R    G  R +  + RP+IGI  T SD++ C+ H  E 
Sbjct: 8   LRSQRWF---APDNMRAFAHRQRTQQTGYKRSDFMN-RPVIGIINTWSDISTCHTHLRER 63

Query: 68  AQRVKAGIRDAGGIPMEFPVHPIAEQSRRPTAALDRNLAYLGLVEILHGYPLDGVVLTTG 127
           AQ VK GI  AGG P+E P   + E   +PT  + RN   +   E+L  +P+DG +L  G
Sbjct: 64  AQFVKEGIIRAGGYPLEMPAMSLGEVMVKPTTMMYRNFLAMETEELLRSHPIDGAILMGG 123

Query: 128 CDKTTPACLMAAATTDLPAIVLSGGPMLDGHHKGELIGSGTVLWHARNLMAAGEIDYEGF 187
           CDKTTP  LM A + ++PAI +  G  L+G+ KG+ IG+GT      +   AG +  E +
Sbjct: 124 CDKTTPGLLMGAISMNIPAIYIPAGASLNGNFKGQKIGTGTHTRKYWDEKRAGNLSDEDW 183

Query: 188 MEMTTAASPSVGHCNTMGTALSMNALAEALGMSLPGCASIPAPYRERGQMAYATGKRICE 247
           +E+    + SVG CNTMGTA S+ ++AE LG  LPG A+IPA      ++    G+RI +
Sbjct: 184 LELEAKMTRSVGTCNTMGTASSLTSMAEVLGFCLPGSATIPAADSAHQRLCSLAGERITK 243

Query: 248 LVLQDIRPSQIMTRQAFENAIAVASALGASSNCPPHLIAIARHMGVELSL---DDWQRIG 304
           +V +D+ P  + T++AFENAI    ALG S+N   HLIA+A   G++L +   D+W R  
Sbjct: 244 MVFEDLTPKSLATKEAFENAIVTYVALGGSTNGIIHLIAMAGRAGIDLPMSLFDEWAR-- 301

Query: 305 EDVPLLVNCMPAGKYLGEGFHRAGGVPSVMHELQKAGRLHEDCATVSGRTIGEIVSSSLT 364
             VP++ N MPAG+YL E    AGG+P+++  L+    LH D   V+GRT+GE +     
Sbjct: 302 -KVPVIANLMPAGEYLMEDLFYAGGLPALLTRLKDF--LHLDQGNVNGRTLGENLEGVEI 358

Query: 365 SNADVIHPFDTPLKHRAGFIVLSGNFF-DSAIMKMSVVGEAFRKTYLSEPGAENSFEARA 423
            N DVI   + P+  R    V++GN   D A+ K S           + P   N    +A
Sbjct: 359 YNEDVIRSLENPVNDRGTIGVITGNLCPDGAVCKPSA----------ASPHLLN-HRGKA 407

Query: 424 IVFEGPEDYHARIDDPALDIDERCILVIRGVGTVGYPGSAEVVNMAPPAALIKQGIDSLP 483
           +VFE     +A+IDDP LD+DE  +LV++  G +G PG  E   +  P  L+++G+  + 
Sbjct: 408 LVFENHAQMNAQIDDPNLDVDENTVLVLKNAGPIGGPGMPEWGGLPIPKKLLQKGVRDMV 467

Query: 484 CLGDGRQSGTSASPSILNMSPEAAVGGGLALLQTNDRLKVDLNTRTVNLLIDDEEMARRR 543
            + D R SGT     IL+++PE+ VGG LA +QT D + +D+  RT+NL I DEEMA R+
Sbjct: 468 RISDARMSGTHYGTCILHVAPESYVGGPLAFVQTGDWIALDVEKRTLNLEISDEEMAARK 527

Query: 544 LEWTPNIPPSQTPWQELYRQLVGQLSTG 571
             W P  P     +  ++   V Q + G
Sbjct: 528 AAWAPPPPKFARGYGAMFSTHVTQANEG 555


Lambda     K      H
   0.319    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 915
Number of extensions: 48
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 594
Length of database: 573
Length adjustment: 36
Effective length of query: 558
Effective length of database: 537
Effective search space:   299646
Effective search space used:   299646
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources