Alignments for a candidate for bgl in Flavobacterium glycines Gm-149

GapMind for catabolism of small carbon sources

Alignments for a candidate for bgl in Flavobacterium glycines Gm-149

Align β-glucosidase (BglF3;Fjoh_0775) (EC 3.2.1.21) (characterized)
to candidate WP_066328323.1 BLR17_RS01490 glycosyl hydrolase

Query= CAZy::ABQ03809.1
         (745 letters)



>NCBI__GCF_900100165.1:WP_066328323.1
          Length = 744

 Score = 1197 bits (3096), Expect = 0.0
 Identities = 588/745 (78%), Positives = 657/745 (88%), Gaps = 1/745 (0%)

Query: 1   MKNKMIYLSAALVFAFFTSCKNETQTSASNSSQTEEYVGKEISTDHDAEIDKLISQMTLE 60
           MKNK+I LS   +   FT     +QT ++        + K + ++ D  I++LIS+MTLE
Sbjct: 1   MKNKIIRLSVFSIL-LFTGLNANSQTVSNMRLLNARPIEKPLGSEFDTNIEELISKMTLE 59

Query: 61  EKIGMLHGNSMFANAGVKRLGIPELKMADGPLGVREEISRDNWAPAGWTNDFATYYPAGG 120
           EKIGMLHGNSMFAN GV RLGIPELKMADGPLGVREEISRD+W PAG  NDFATYYPA G
Sbjct: 60  EKIGMLHGNSMFANGGVPRLGIPELKMADGPLGVREEISRDSWKPAGLDNDFATYYPASG 119

Query: 121 ALAATWNAEMAHTFGTSLGEELRARDKDMLLSPAINMVRTPLGGRTYEYMSEDPFLNKKI 180
           ALAATWN  MA  FG S+G+ELRARDKDMLLSPAIN+VRTPLGGRTYEYM+EDPFLNKK+
Sbjct: 120 ALAATWNTRMASLFGNSVGQELRARDKDMLLSPAINIVRTPLGGRTYEYMTEDPFLNKKM 179

Query: 181 AVPLVVGLQEKDVMACVKHYAANNQETNRDFVDVQIDERTLREIYLPAFEATVKEAKAYS 240
           AVPLVV LQ+ DVMACVKH+AANNQETNRDFVDVQIDERTLREIYLP FEAT+KE   YS
Sbjct: 180 AVPLVVSLQDNDVMACVKHFAANNQETNRDFVDVQIDERTLREIYLPTFEATIKEGNVYS 239

Query: 241 IMGAYNKFRGEYLCENDYMLNKILRDEWGFKGVVVSDWAAVHSTAKSLKNGLDIEMGTPK 300
           +MGAYNKFRGEYLCENDYMLN ILRDEWGFKGVVVSDWAAVHST KSL+NGLDIEMG+ K
Sbjct: 240 VMGAYNKFRGEYLCENDYMLNTILRDEWGFKGVVVSDWAAVHSTVKSLQNGLDIEMGSKK 299

Query: 301 PFNEFFLADKLIAAVKSGEVSEKEIDLHVKRILRVLFQVKAMGGGERAKGSIATEAHYQD 360
           PFNE+FLADKLIAAVK+GEVSEKEID HVKRIL VLFQVKAM G +R KGS+ATE+HYQD
Sbjct: 300 PFNEYFLADKLIAAVKAGEVSEKEIDKHVKRILHVLFQVKAMDGKDRVKGSLATESHYQD 359

Query: 361 AYKIAAEAIILLKNENNALPLKLDGVKSIAVIGNNATKKNALGGFGAGVKTKREVTPLEG 420
           AY+IA+EA++LLKN NNALPLKLDG+KSIAVIGNNATKKNALGGFGAGVKTKRE+TPLEG
Sbjct: 360 AYEIASEAVVLLKNNNNALPLKLDGIKSIAVIGNNATKKNALGGFGAGVKTKREITPLEG 419

Query: 421 LKNRLPSSVKINYAEGYLEKYEEKNKGNLGNITSTGPVTIDKLDPAKVQEAVEAAKKSDV 480
           LKNRLP+S+KINYAEGYLE+YE+KNKGNLGNITS GPVTID+LD AK+QEA+EA K S  
Sbjct: 420 LKNRLPASIKINYAEGYLERYEKKNKGNLGNITSKGPVTIDQLDDAKLQEALEAVKNSGA 479

Query: 481 AIIFAGSNRDYETEASDRRDLHLPFGQEELIKKVIEANPKTIVVMIAGAPFDLNEVSQKS 540
           AIIFAGSNRDYETEASDR+DL LPFGQEELIKKV+  NPKTIVVMIAGAPF++ E+S+K+
Sbjct: 480 AIIFAGSNRDYETEASDRKDLKLPFGQEELIKKVLAINPKTIVVMIAGAPFEMEEISKKT 539

Query: 541 SALVWSWFNGSEGGNALADVILGKVNPSGKLPWTMPKQLKDSPAHATNSFPGDKAVNYAE 600
           +ALVWSWFNGSEGGNALADV+LGKVNPSGKLPWTMPK+L DSPAHATNSFPGDK VNYAE
Sbjct: 540 NALVWSWFNGSEGGNALADVLLGKVNPSGKLPWTMPKKLMDSPAHATNSFPGDKTVNYAE 599

Query: 601 GILIGYRWFDTKNVAPLYPFGYGLSYTTFALDNAKTDKDSYAQNDVIEVTVDVKNTGKVD 660
           GIL+GYRWFDTK ++P+YPFGYGLSYT F    A +DK+ Y  ++VI + VD+KNTGKVD
Sbjct: 600 GILVGYRWFDTKKISPMYPFGYGLSYTNFTFGKAFSDKEIYNNDEVITIAVDLKNTGKVD 659

Query: 661 GKEVVQLYTSKSDSKITRAAQELKGFKKADVKAGGSEKITIKVPVKELAYYDVAAKKWTV 720
           GKEVVQLY+SK+DSKI RAAQELKGF K  VKAG ++K+ IKVPVKELAYYDVA KKWTV
Sbjct: 660 GKEVVQLYSSKTDSKIDRAAQELKGFTKILVKAGATQKVIIKVPVKELAYYDVANKKWTV 719

Query: 721 EPGKYTIKLGTSSRDIKKEINFTIK 745
           EPGKY +KLG SSRDIKKEI+  IK
Sbjct: 720 EPGKYKLKLGNSSRDIKKEIDVIIK 744


Lambda     K      H
   0.314    0.131    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1786
Number of extensions: 65
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 745
Length of database: 744
Length adjustment: 40
Effective length of query: 705
Effective length of database: 704
Effective search space:   496320
Effective search space used:   496320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources