GapMind for catabolism of small carbon sources

 

Alignments for a candidate for galdh in Flavobacterium glycines Gm-149

Align Probable galactose dehydrogenase GalD; EC 1.1.1.- (characterized)
to candidate WP_066326091.1 BLR17_RS14060 3-oxoacyl-[acyl-carrier-protein] reductase

Query= SwissProt::Q92RN6
         (256 letters)



>NCBI__GCF_900100165.1:WP_066326091.1
          Length = 248

 Score =  138 bits (347), Expect = 1e-37
 Identities = 88/246 (35%), Positives = 138/246 (56%), Gaps = 4/246 (1%)

Query: 11  LRDRGVLVTGGGSGIGAALVEAFARQGARVAFV-DIAAESSLALCEKVAAQTGQAPHFIQ 69
           L  +  ++TG   GIG  + E FA+ GA VAF    +AES+LAL  ++ A   +A  + +
Sbjct: 4   LEGKVAIITGASRGIGRGIAEVFAKNGADVAFTYSSSAESALALENELNALGVKAKGY-K 62

Query: 70  ADLRNVEAVRAAADEAVAKLGSVRVLVNNAARDDRQALEAVTEESWDESLSVNLRHLFFM 129
           ++  + +  +   D  +A  G+V VL+NNA       L  ++E  +D+ + VNL+ +F M
Sbjct: 63  SNAADFDEAQTLVDAVLADFGTVDVLINNAGITKDNLLMRMSEADFDQVIDVNLKSVFNM 122

Query: 130 CQAVAPHMQRQGGGSIVNFSSIAFLLNMPEIPAYSTAKAGIIGLTKSLAGKLGPDNIRVN 189
            +A+     ++  GSI+N SS+  +        Y+ +KAG IG TKS+A +LG  NIR N
Sbjct: 123 TKAIQKTFLKKRAGSIINISSVVGVSGNAGQTNYAASKAGAIGFTKSVALELGSRNIRCN 182

Query: 190 AILPGMIVTERQRRLWLTEESIARMQERQCLKRMLVADDLVGPCLFLASDSSAAMTAQAM 249
           AI PG I TE   +  L+E+ +   ++   LKR    +D+   CLFLASD SA +T Q +
Sbjct: 183 AIAPGFIETEMTAK--LSEDVVKGWRDGIPLKRGGSPEDVANACLFLASDMSAYVTGQVL 240

Query: 250 IIDGGV 255
            + GG+
Sbjct: 241 NVCGGM 246


Lambda     K      H
   0.321    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 115
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 256
Length of database: 248
Length adjustment: 24
Effective length of query: 232
Effective length of database: 224
Effective search space:    51968
Effective search space used:    51968
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory