Protein WP_090664757.1 in Belnapia rosea CPCC 100156
Annotation: NCBI__GCF_900101615.1:WP_090664757.1
Length: 378 amino acids
Source: GCF_900101615.1 in NCBI
Candidate for 32 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| lactose catabolism | lacK | hi | LacK, component of Lactose porter (characterized) | 45% | 100% | 292.4 | sn-glycerol-3-phosphate import ATP-binding protein UgpC; EC 7.6.2.10 | 46% | 281.6 |
| D-mannitol catabolism | mtlK | med | SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized) | 45% | 100% | 280 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-sorbitol (glucitol) catabolism | mtlK | med | MtlK, component of The polyol (mannitol, glucitol (sorbitol), arabitol (arabinitol; lyxitol)) uptake porter, MtlEFGK (characterized) | 43% | 100% | 273.1 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| sucrose catabolism | thuK | med | ABC transporter (characterized, see rationale) | 42% | 93% | 267.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| trehalose catabolism | thuK | med | Trehalose import ATP-binding protein SugC; EC 7.5.2.- (characterized) | 48% | 75% | 267.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | med | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 43% | 100% | 265 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | med | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 43% | 100% | 265 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | thuK | med | Trehalose/maltose import ATP-binding protein MalK; EC 7.5.2.1 (characterized) | 44% | 94% | 264.2 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| xylitol catabolism | Dshi_0546 | med | ABC transporter for Xylitol, ATPase component (characterized) | 44% | 100% | 262.7 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | malK | med | ABC-type maltose transporter (subunit 3/3) (EC 7.5.2.1) (characterized) | 42% | 97% | 261.5 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-cellobiose catabolism | gtsD | med | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 43% | 95% | 259.6 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-glucose catabolism | gtsD | med | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 43% | 95% | 259.6 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| lactose catabolism | gtsD | med | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 43% | 95% | 259.6 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | gtsD | med | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 43% | 95% | 259.6 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| sucrose catabolism | gtsD | med | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 43% | 95% | 259.6 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| trehalose catabolism | gtsD | med | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 43% | 95% | 259.6 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-glucosamine (chitosamine) catabolism | SM_b21216 | med | ABC transporter for D-Glucosamine, ATPase component (characterized) | 42% | 99% | 258.8 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-xylose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 41% | 96% | 253.8 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | aglK | med | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 41% | 100% | 253.1 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| sucrose catabolism | aglK | med | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 41% | 100% | 253.1 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| trehalose catabolism | aglK | med | ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) | 41% | 100% | 253.1 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-galactose catabolism | PfGW456L13_1897 | med | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 41% | 97% | 252.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | malK_Bb | med | ABC-type maltose transport, ATP binding protein (characterized, see rationale) | 43% | 87% | 237.7 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-cellobiose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 40% | 96% | 235.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-glucose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 40% | 96% | 235.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| lactose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 40% | 96% | 235.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 40% | 96% | 235.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| sucrose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 40% | 96% | 235.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| trehalose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 40% | 96% | 235.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| D-maltose catabolism | malK_Sm | lo | MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 37% | 99% | 228.4 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| trehalose catabolism | malK | lo | MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 37% | 99% | 228.4 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
| glycerol catabolism | glpS | lo | ABC transporter for Glycerol, ATPase component 1 (characterized) | 35% | 98% | 188.3 | ABC transporter for Lactose, ATPase component | 43% | 282.0 |
Sequence Analysis Tools
View WP_090664757.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MAGITISGLTKRFGVTQVLRGVSLAVAPGEFVALVGPSGCGKTTLMRIIAGIETADAGEV
VIGERAVTRLRPAKRDIAMVFQSYALYPHLTVRENIAVPLAMRRLSAWQRLPLLGGLLPG
ARARRAAIAEEVVRAAAPLGLEPLLDRRPGQLSGGQRQRVALARAIIRRPAAFLMDEPLS
NLDAALRVQTRREIVEIHRAVGVATLYVTHDQAEALTMADKVAVMMGGELLQVAPPEAIY
AEPADLRVAGFIGSPRINTLAAAVDAAGRVTVAGLPVGLRTEARGALTLALRPEALRPAA
EGLPAIAEHLEFLGDGLLLHARHTPDGAPLILRLEPEAREGLRPGAALRLGFAPSRALLF
GADGRRVAARADAPLVAA
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory