Alignments for a candidate for prpD in Belnapia rosea CPCC 100156

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpD in Belnapia rosea CPCC 100156

Align 2-methylcitrate dehydratase; 2-MC dehydratase; Aconitate hydratase; ACN; Aconitase; EC 4.2.1.79; EC 4.2.1.3 (characterized)
to candidate WP_090568919.1 BLR02_RS19020 MmgE/PrpD family protein

Query= SwissProt::H8F0D6
         (505 letters)



>NCBI__GCF_900101615.1:WP_090568919.1
          Length = 512

 Score =  618 bits (1593), Expect = 0.0
 Identities = 313/515 (60%), Positives = 377/515 (73%), Gaps = 18/515 (3%)

Query: 5   MLMHAVRAWRSADDFPCTEHMAYKIAQVAADPVDVDPEVADMVCNRIIDNAAVSAASMVR 64
           M  HAV+ + S       + +A+KIA VAAD V V+ +VA M+ NRIIDNA+V+ A++ R
Sbjct: 1   MQKHAVKLYPSKSLLKREDQLAWKIAGVAADKVAVEKDVAGMIINRIIDNASVAIAAINR 60

Query: 65  RPVTVARHQALAHPVRHGAKVFGVEGS--YSADWAAWANGVAARELDFHDTFLAADYSHP 122
           RPVT AR  AL HP + GA +FGV  +  +S +WAAWANG A RELD HDTFLAADYSHP
Sbjct: 61  RPVTSARTMALGHPRKGGATIFGVGANKRFSPEWAAWANGTAVRELDMHDTFLAADYSHP 120

Query: 123 ADNIPPLVAVAQQLGVCGAELIRGLVTAYEIHIDLTRGICLHEHKIDHVAHLGPAVAAGI 182
            DNIPP++AVAQ +   G +LIRGL T YEI IDL + ICLHEHK+DH+AHL P+ AAGI
Sbjct: 121 GDNIPPILAVAQTMEKSGRDLIRGLATGYEIQIDLVKAICLHEHKVDHIAHLCPSAAAGI 180

Query: 183 GTMLRLDQETIYHAIGQALHLTTSTRQSRKGAISSWKAFAPAHAGKVGIEAVDRAMRGEG 242
           GT+L+L QETIY A+ QALH++ S RQSRKG ISSWKAFAPAHAGK+ +EAVDRAMRGEG
Sbjct: 181 GTLLKLPQETIYQAVQQALHVSISFRQSRKGEISSWKAFAPAHAGKLAVEAVDRAMRGEG 240

Query: 243 SPAPIWEGEDGVIAWLLAGPEHT-------------YRVPLPAPGEPKRAILDSYTKQHS 289
           +P+PI+EGED VI W+L+G                 Y VPLP  GE KRAILDSYTK+HS
Sbjct: 241 APSPIYEGEDSVIGWVLSGRTVADKEGRAAAYEPVYYEVPLPGAGEAKRAILDSYTKEHS 300

Query: 290 AEYQSQAPIDLACRLRERIGDLDQIASIVLHTSHHTHVVIGTGSGDPQKFDPDASRETLD 349
           AEYQSQA IDLA R+R+RI D++QI  IV+HTSHHTH VIGTG+ DPQK DP ASRETLD
Sbjct: 301 AEYQSQALIDLAFRMRDRIEDMEQIEKIVIHTSHHTHYVIGTGANDPQKMDPKASRETLD 360

Query: 350 HSLPYIFAVALQDGCWHHERSYAPERARRSDTVALWHKISTVEDPEWTRRYHCADPAKKA 409
           HS+ YIFAVALQDG WHH  SYAP RA R+DTV LW KI T E+ EWTRRYH  DP + +
Sbjct: 361 HSIMYIFAVALQDGRWHHVDSYAPRRAARADTVRLWQKIETREEAEWTRRYHSRDPNELS 420

Query: 410 FGARAEVTLHSGEVIVDELAVADAHPLGTRPFERKQYVEKFTELADGVVEPVEQQRFLAV 469
           FG R E+T+  G V+ DELAVA+AHPLG +PF R+ Y+ KF  L DG++   E  RFLA 
Sbjct: 421 FGGRVEITMKDGSVLSDELAVANAHPLGAKPFGREDYINKFQILTDGIISARESNRFLAD 480

Query: 470 VESLADLESGAVGGLNVLVDPRVLDKAPVIPPGIF 504
           V+ LA + +G +  LNV +    L +     PGIF
Sbjct: 481 VQDLARIPAGELHVLNVALPNGALAEG---KPGIF 512


Lambda     K      H
   0.320    0.135    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 754
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 505
Length of database: 512
Length adjustment: 34
Effective length of query: 471
Effective length of database: 478
Effective search space:   225138
Effective search space used:   225138
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources