Protein WP_090273180.1 in Pseudomonas litoralis 2SM5
Annotation: NCBI__GCF_900105005.1:WP_090273180.1
Length: 426 amino acids
Source: GCF_900105005.1 in NCBI
Candidate for 9 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-malate catabolism | dctM | lo | C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) | 34% | 98% | 262.7 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| fumarate catabolism | dctM | lo | C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) | 34% | 98% | 262.7 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| succinate catabolism | dctM | lo | C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) | 34% | 98% | 262.7 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| D-gluconate catabolism | gntB | lo | TRAP-type large permease component (characterized, see rationale) | 34% | 100% | 248.8 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| D-glucuronate catabolism | dctM | lo | Putative TRAP dicarboxylate transporter, DctM subunit (characterized, see rationale) | 32% | 97% | 221.9 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| L-glutamate catabolism | gtrB | lo | GtrB aka SLL1103, component of Tripartite glutamate:Na+ symporter (characterized) | 31% | 98% | 204.1 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| 4-hydroxybenzoate catabolism | fcbT3 | lo | FcbT3, component of Tripartite 4-chlorobenzoate symporter (also binds and may transport 4-bromo-, 4-iodo-, and 4-fluorobenzoate and with a lower affinity, 3-chlorobenzoate, 2-chlorobenzoate, 4-hydroxybenzoate, 3-hydroxybenzoate, and benzoate) (characterized) | 30% | 96% | 202.6 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| pyruvate catabolism | dctM | lo | Alr3027 protein, component of The 2-oxo monocarboxylate transporter (Pernil et al., 2010). Transports pyruvate which is inhibited by various 2-ketoacids (characterized) | 30% | 97% | 182.6 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
| 2-oxoglutarate (alpha-ketoglutarate) catabolism | dctM | lo | TRAP transporter, subunit DctM (characterized, see rationale) | 31% | 81% | 164.5 | Ectoine TRAP transporter large permease protein TeaC | 62% | 533.9 |
Sequence Analysis Tools
View WP_090273180.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MLTLTLIITVLLLLLGFPMMFPLLAASLMLLFLVLQMDNVGLLIGQMISGVQTWALAAVP
LFIFAADLMTRGRTANRLLDLVQSFVGHLRGGLPITTAASCTLFGAVSGSTQATVVAMGG
PMRPKLLEKGYSDKFSIALIINASDIALLIPPSIGLIIYGVVSGASIGDLFIAGILPGLL
LLLLFSAYCWIASIRMGIEPEPKATWRYRWQALKKAALPLGFPLVVVGGIYGGLFSPTEA
AAIAVLYAVVLEIVILRSVKVTELWDIALSTGLITAVVFILVAAGNAFTYTISFAGIPQA
ILNPIIADIGHSQVMVLSLIAVSFFIGCMFVDPIVVILILTPLFKPAVAAAGLDPVHVGI
IVTLQAAIGSATPPFGCDIFTAMAIFRRPYFDVIRGTPPFFALLVLATIILILFPSISLW
LPSLAS
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory