GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bgtA in Pseudomonas litoralis 2SM5

Align BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized)
to candidate WP_090275879.1 BLU11_RS18455 methionine ABC transporter ATP-binding protein

Query= TCDB::P73721
         (252 letters)



>NCBI__GCF_900105005.1:WP_090275879.1
          Length = 334

 Score =  172 bits (435), Expect = 1e-47
 Identities = 104/250 (41%), Positives = 141/250 (56%), Gaps = 7/250 (2%)

Query: 8   LISFDQLQKNFGA----LQVLRGVTGEIYPKDVISIIGPSGCGKSTFLRCLNRLEPISGG 63
           +I F Q+ K +      +  L      I    V  IIG SG GKST LR +NRLE  S G
Sbjct: 1   MIEFQQVNKAYRVGGRDIPALHATDLTIEAGQVYGIIGHSGAGKSTLLRLINRLEEPSNG 60

Query: 64  RLEVAGVDLSGAKIDQKHLRQLRVRVGMVFQHFNLFPHLTVLQNLLLAPRKVLRIPMAEA 123
           R+ VAG D +   ++ + LR+ R RVGM+FQHFNL    TV  N+ L  R        + 
Sbjct: 61  RIMVAGEDTTA--LNAEGLRRFRQRVGMIFQHFNLLSSATVADNVALPLRLAGSANRQQI 118

Query: 124 KDRALTYLDKVGLGTKADNYPDQLSGGQKQRVAIARGLCMKPEILLFDEPTSALDPELVG 183
             R  + LD+VGL   A  YP QLSGGQKQRV IAR L  +P ILL DE TSALDP   G
Sbjct: 119 AARVASLLDRVGLSDHASKYPAQLSGGQKQRVGIARALATEPSILLCDEATSALDPHTTG 178

Query: 184 EVLNVMKQLAEE-GMTMAVVTHEMQFAREVSNRVFFFNQGIIEEEGDPNEVFRNPKSDRL 242
           +VL ++ ++  E  +T+ ++THEM   R V ++V   + G I E+G   +VF +P+    
Sbjct: 179 QVLQLLSEINRELNLTIVLITHEMDVIRRVCDQVAVMDGGRIVEQGTVADVFLHPQHPTT 238

Query: 243 RAFLSRIQSS 252
           R+F+   +S+
Sbjct: 239 RSFVFGSESA 248


Lambda     K      H
   0.321    0.139    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 218
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 252
Length of database: 334
Length adjustment: 26
Effective length of query: 226
Effective length of database: 308
Effective search space:    69608
Effective search space used:    69608
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory