GapMind for catabolism of small carbon sources

 

Alignments for a candidate for AZOBR_RS08250 in Pseudomonas litoralis 2SM5

Align Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate WP_090272108.1 BLU11_RS03755 ABC transporter ATP-binding protein

Query= uniprot:G8ALJ1
         (236 letters)



>NCBI__GCF_900105005.1:WP_090272108.1
          Length = 256

 Score =  125 bits (314), Expect = 8e-34
 Identities = 77/248 (31%), Positives = 136/248 (54%), Gaps = 16/248 (6%)

Query: 1   MLKVSGVHTFYGAIEALKGVDIEIGAGEIVSLIGANGAGKSTLLMTICGSPRARMGRITF 60
           +L VSG+   +G + A+  V +++  G+IVSLIG NGAGK+T+   + G  +   G I F
Sbjct: 6   LLDVSGLCMRFGGLLAVDQVALQVEEGQIVSLIGPNGAGKTTVFNCLTGFYKPSAGSIRF 65

Query: 61  EGQDITQMPTYELVRLGIAQSPEGRRIFPRMSVLENL-----------QMGSITAKPGSF 109
            G++++ MP ++L R+G+ ++ +  R+F +MSV+ENL            +  +   P   
Sbjct: 66  HGEEVSGMPGFKLARMGMVRTFQHVRLFKQMSVVENLLVAQHHHMNTNMLAGLLKTPSFR 125

Query: 110 ANE---LERVLTLFPR--LKERISQRAGTMSGGEQQMLAIGRALMSQPRLLLLDEPSLGL 164
            +E   L+R      R  L E  ++ AGT++ G+Q+ L I R +++QPRLL+LDEP+ GL
Sbjct: 126 RSEKEALDRAAQWLERTGLLEFANRTAGTLAYGQQRRLEIARCMVTQPRLLMLDEPAAGL 185

Query: 165 APLVVKQIFQAVKDINREQKMTVFMVEQNAFHALKLAHRGYVMVNGKVTMSGTGAELLAN 224
            P     +   + ++  E  +T+ ++E +    + ++    V+  G    +GT  ++  +
Sbjct: 186 NPRETVDLQALIAELRSEHGLTILLIEHDMKLVMGISDHVVVINQGCPLAAGTPEQVCRD 245

Query: 225 EEVRSAYL 232
            EV  AYL
Sbjct: 246 PEVIKAYL 253


Lambda     K      H
   0.320    0.136    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 136
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 236
Length of database: 256
Length adjustment: 24
Effective length of query: 212
Effective length of database: 232
Effective search space:    49184
Effective search space used:    49184
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory