Alignments for a candidate for prpE in Pseudomonas litoralis 2SM5

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Pseudomonas litoralis 2SM5

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate WP_090271795.1 BLU11_RS01910 acetate--CoA ligase

Query= SwissProt::P27550
         (652 letters)



>NCBI__GCF_900105005.1:WP_090271795.1
          Length = 647

 Score =  826 bits (2133), Expect = 0.0
 Identities = 392/647 (60%), Positives = 496/647 (76%), Gaps = 7/647 (1%)

Query: 1   MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQG-KILDWIKPYQKVKNTS 59
           M +I  H +  N   +  ++   YE MY+QSI  PD FW EQ  + ++W++P+ KV  ++
Sbjct: 1   MYKIQNHKVSENQLRQTHLDQDGYERMYRQSIEQPDMFWAEQANRFVEWMQPWSKVHESN 60

Query: 60  FAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDASQSKHISYKELHRDVCR 119
              G  +  W+ D  LN++ NC+DRHL+   D+TAIIWEGDD + S++I+Y+ELH +VCR
Sbjct: 61  MEKGEAA--WFVDAKLNVSVNCIDRHLKTRADQTAIIWEGDDPNDSRYITYRELHEEVCR 118

Query: 120 FANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSN 179
            AN L E G+KKGD V +YMPM+PEAA AMLACARIGAVH+V+FGGFSPEA+  RI ++ 
Sbjct: 119 LANVLKERGVKKGDRVCLYMPMIPEAAFAMLACARIGAVHTVVFGGFSPEALRDRIQNAE 178

Query: 180 SRLVITSDEGVRAGRSIPLKKNVDDALKN-PNVTSVEHVVVLKRTGGKIDWQEGRDLWWH 238
             +VIT+DEGVR G+++PLK+NVD AL   PNV +V   V +KRTG  + W E +D+W+H
Sbjct: 179 CDVVITADEGVRGGKTVPLKQNVDKALTACPNVHTV---VTVKRTGADVAWDEQKDVWYH 235

Query: 239 DLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPG 298
           +   Q S +   E M++EDPLFILYTSGSTGKPKGVLHTTGGYLV  ALT  YVFDYH G
Sbjct: 236 EACAQVSKECTPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGYLVSVALTHHYVFDYHDG 295

Query: 299 DIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAP 358
           +++WCTADVGWVTGHSY++YGPL  GATTLMFEGVP +PT +R  +VVDKH+VN+ YTAP
Sbjct: 296 EVFWCTADVGWVTGHSYIVYGPLCNGATTLMFEGVPTYPTASRCWEVVDKHKVNVFYTAP 355

Query: 359 TAIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTET 418
           TAIRALMA G++ ++ + R S+R+LGSVGEPINPE WEWY++ +G+ +CP+VDTWWQTET
Sbjct: 356 TAIRALMALGEEPVKSSSRESIRLLGSVGEPINPEVWEWYYQVVGDSRCPIVDTWWQTET 415

Query: 419 GGFMITPLPGATELKAGSATRPFFGVQPALVDNEGNPLEGATEGSLVITDSWPGQARTLF 478
           G  +I PLPGA  LK G+A RP FGV PALVDNEGN L+G  +G+LVI  SWP Q RT++
Sbjct: 416 GSILIAPLPGACGLKPGAAMRPMFGVVPALVDNEGNELDGVADGNLVIKQSWPSQIRTVY 475

Query: 479 GDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVA 538
           GDH R  +TYFST+K +YF+GDGARRD DG YWITGRVDDVLN+SGHR+GTAE+ESALV 
Sbjct: 476 GDHHRLIETYFSTYKGVYFTGDGARRDADGDYWITGRVDDVLNISGHRIGTAEVESALVL 535

Query: 539 HPKIAEAAVVGIPHNIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHW 598
           +  +AEAAVVG PH+IKGQAIYAYV    G   + E+ AE++  V +++G  A PD +  
Sbjct: 536 NDYVAEAAVVGCPHDIKGQAIYAYVMPMKGVTLTDEVMAELKVAVSEQVGAFARPDFIQP 595

Query: 599 TDSLPKTRSGKIMRRILRKIAAGDTSNLGDTSTLADPGVVEKLLEEK 645
           T  LPKTRSGKIMRRILRKIA  +   LGDTSTLADPGVV++L+E +
Sbjct: 596 TTGLPKTRSGKIMRRILRKIACDELDTLGDTSTLADPGVVDELVEHR 642


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1360
Number of extensions: 52
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 647
Length adjustment: 38
Effective length of query: 614
Effective length of database: 609
Effective search space:   373926
Effective search space used:   373926
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources