Align ABC transporter for D-Sorbitol, ATPase component (characterized)
to candidate WP_090274680.1 BLU11_RS14815 polyamine ABC transporter ATP-binding protein
Query= reanno::acidovorax_3H11:Ac3H11_2941 (350 letters) >NCBI__GCF_900105005.1:WP_090274680.1 Length = 378 Score = 222 bits (565), Expect = 1e-62 Identities = 121/322 (37%), Positives = 187/322 (58%), Gaps = 17/322 (5%) Query: 3 YLQLRGIEKFFGEHRAIKGIDLTIQQGEFIVFVGPSGCGKSTLLRLIAGLEAIDGGSLML 62 ++++ + K F + A+ + LTI +GE +G SG GKSTLLR++AG E G ++L Sbjct: 20 FVKIERVSKQFDDALAVDDVTLTINRGEIFALLGGSGSGKSTLLRILAGFETPSEGRVLL 79 Query: 63 DGRDITDQPSSKRDLAMVFQSYALYPHMSVYENMSFALKLAKVDKQVIDEKVQNAARILN 122 DG++IT P KR + M+FQSYAL+PHM+V +N++F LK K+ I E+V ++++ Sbjct: 80 DGQNITALPPHKRPINMMFQSYALFPHMTVEQNIAFGLKQDKLSNTEISERVAEMLKLVH 139 Query: 123 LTQYLQRTPKELSGGQRQRVAIGRAIVRAPKVFLFDEPLSNLDAALRGQTRVEIAKLHRD 182 + +Y +R P +LSGGQRQRVA+ R++ + PK+ L DEP+ LD LR Q ++E+ ++ Sbjct: 140 MAKYAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRSQMQLELVEIIER 199 Query: 183 LGATTIYVTHDQVEAMTLADRVVVLRDGIIEQVGTPLELYDKPANQFVAQFIGT------ 236 +G T I VTHDQ EAMT+A R+ ++ G I QVGTP+++Y+ P N+ VA+F+G+ Sbjct: 200 VGVTCIMVTHDQEEAMTMAQRIAIMDQGWIVQVGTPMDIYESPVNRHVAEFVGSVNIFEG 259 Query: 237 ---PQMNVVPVDKLPQPVQQ----QAPAAPAGAAVGAIGLRPENITVRTTGATP----VG 285 M+ + + PQ +Q A LRPE + V T Sbjct: 260 EIVADMDDHVIIECPQLDRQIYLGHGVTTRAEDKSAIYALRPEKVFVTTEQPEQPYNWAH 319 Query: 286 GQVDLIEALGAETLIYVTTPGG 307 G+V I LG ++ Y+ G Sbjct: 320 GEVHDIAYLGGHSVYYIKLDSG 341 Lambda K H 0.320 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 324 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 350 Length of database: 378 Length adjustment: 29 Effective length of query: 321 Effective length of database: 349 Effective search space: 112029 Effective search space used: 112029 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory