Alignments for a candidate for iolA in Pseudomonas litoralis 2SM5

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolA in Pseudomonas litoralis 2SM5

Align malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized)
to candidate WP_090275928.1 BLU11_RS18585 CoA-acylating methylmalonate-semialdehyde dehydrogenase

Query= BRENDA::A0A081YAY7
         (498 letters)



>NCBI__GCF_900105005.1:WP_090275928.1
          Length = 496

 Score =  726 bits (1873), Expect = 0.0
 Identities = 350/496 (70%), Positives = 408/496 (82%)

Query: 1   MTLIKHLIGGELIADTGRTADVFNPSTGEAVRKVPLADRETMQQAIDAAKAAFPAWRNTP 60
           M  I HLI G +   + RT DVFNPSTGE  +K+ +A + T++ AI AA+AA+PAWRNTP
Sbjct: 1   MKTIGHLINGTIDTQSSRTQDVFNPSTGEVSKKLAVASKPTVEAAIAAAQAAYPAWRNTP 60

Query: 61  PAKRAQVLFRFKQLLEANEERIVKLISEEHGKTIEDAAGELKRGIENVEYATAAPEILKG 120
           P KRA+V+FRFK+LLE N + I +LI EEHGK   DA GEL+RGIENVEYA  APE+LKG
Sbjct: 61  PLKRAKVMFRFKELLERNADEICRLIGEEHGKIAHDAMGELQRGIENVEYACGAPELLKG 120

Query: 121 EYSRNVGPNIDAWSDFQPIGVVAGITPFNFPAMVPLWMYPLAIACGNTFILKPSERDPSS 180
           E+S+N GP ID+WS+FQP+GVVAGITPFNFPAMVPLWMYP+AI CGNTF+LKPSER+PS+
Sbjct: 121 EFSKNAGPAIDSWSEFQPLGVVAGITPFNFPAMVPLWMYPMAIVCGNTFVLKPSERNPSA 180

Query: 181 TLLIAELFHEAGLPKGVLNVVHGDKGAVDALIEAPEVKALSFVGSTPIAEYIYSEGTKRG 240
            L IAEL HEAGLP GVLNVV+GDK AVD L+    V+A+SFVGSTPIAEYIY+  +  G
Sbjct: 181 VLFIAELLHEAGLPPGVLNVVNGDKEAVDVLLSDARVQAVSFVGSTPIAEYIYTTASAHG 240

Query: 241 KRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQIADALVQK 300
           KR QALGGAKNHA++MPDAD+DNAVSAL+GAA+GS GERCMA+SVAV VGD+ AD+L+ K
Sbjct: 241 KRCQALGGAKNHAIVMPDADMDNAVSALVGAAFGSSGERCMALSVAVAVGDEAADSLISK 300

Query: 301 LVPQIKGLKIGAGTSCGLDMGPLVTGAARDKVTGYIDTGVAQGAELVVDGRGYKVAGHEN 360
           +  Q+K LK+GA +    D GPL+T A ++KVTGYI +   QGA LVVDGR   VAG++ 
Sbjct: 301 MTEQMKSLKVGAFSDKSNDFGPLITRAHQEKVTGYISSAEQQGATLVVDGRQPAVAGYDK 360

Query: 361 GFFLGGTLFDRVTPEMTIYKEEIFGPVLCIVRVNSLEEAMQLINDHEYGNGTCIFTRDGE 420
           GF++GGTL D VT EMT YKEEIFGPVL ++RVNS+EEAMQLINDHEYGNGTCIFTRDGE
Sbjct: 361 GFYVGGTLIDNVTAEMTCYKEEIFGPVLLVMRVNSMEEAMQLINDHEYGNGTCIFTRDGE 420

Query: 421 AARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYTKRKAITQR 480
           AAR F D I VGMVG+NVPLPVPVAYHSFGGWKRSLFGDLHAYGPD VRFYTKRK ITQR
Sbjct: 421 AARYFSDNIMVGMVGINVPLPVPVAYHSFGGWKRSLFGDLHAYGPDAVRFYTKRKTITQR 480

Query: 481 WPQRKSHEAAQFAFPS 496
           WP     E AQF+FPS
Sbjct: 481 WPSAGVREGAQFSFPS 496


Lambda     K      H
   0.319    0.137    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 807
Number of extensions: 19
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 498
Length of database: 496
Length adjustment: 34
Effective length of query: 464
Effective length of database: 462
Effective search space:   214368
Effective search space used:   214368
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources