Alignments for a candidate for prpB in Pseudomonas litoralis 2SM5

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpB in Pseudomonas litoralis 2SM5

Align Methylisocitrate lyase (EC 4.1.3.30) (characterized)
to candidate WP_090272481.1 BLU11_RS05815 methylisocitrate lyase

Query= reanno::pseudo6_N2E2:Pf6N2E2_6061
         (294 letters)



>NCBI__GCF_900105005.1:WP_090272481.1
          Length = 294

 Score =  426 bits (1096), Expect = e-124
 Identities = 212/292 (72%), Positives = 242/292 (82%)

Query: 3   NSTPGQRFRDAVANEHPLQVVGTINANHALLAKRAGFKAIYLSGGGVAAGSLGVPDLGIT 62
           N TPGQRFR A+A E PLQV+G INANHALLAKRAGFKAIYLSGGGVAAGSLG+PDLGI 
Sbjct: 2   NKTPGQRFRQALAEEQPLQVIGAINANHALLAKRAGFKAIYLSGGGVAAGSLGLPDLGIN 61

Query: 63  GLDDVLTDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVKSMIKFGAAAIHIEDQVGAKR 122
            L+DVL DVRRITDVCDLPL+VD+DTGFG SAFN+ RTVKS+IK GAAA HIEDQVGAKR
Sbjct: 62  TLEDVLIDVRRITDVCDLPLMVDIDTGFGPSAFNIERTVKSLIKAGAAAAHIEDQVGAKR 121

Query: 123 CGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALDRAAACIEAGA 182
           CGHRP KEIVS +EMVDR++AA DA+TD  F ++ARTDA+  EG+E+AL+R    +EAGA
Sbjct: 122 CGHRPGKEIVSTEEMVDRVRAAADAKTDPDFFLIARTDAIQAEGVEAALERCKRYVEAGA 181

Query: 183 DMIFPEAITELEMYKLFASRVKAPILANITEFGATPLYTTEQLAGADVSLVLYPLSAFRA 242
           D IF EA  +L  Y+ F   +  P+LANITEFGATPL+T E+LA   V++ LYPLSAFRA
Sbjct: 182 DAIFAEAAYDLPTYERFVKELNVPVLANITEFGATPLFTREELASVGVAIQLYPLSAFRA 241

Query: 243 MNKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK 294
            NKAAENVY A+R +G QQNVID+MQTR ELYDRI YH FE KLDALFAA K
Sbjct: 242 ANKAAENVYNAVRTEGHQQNVIDSMQTREELYDRIGYHVFEGKLDALFAAGK 293


Lambda     K      H
   0.320    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 277
Number of extensions: 2
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 294
Length of database: 294
Length adjustment: 26
Effective length of query: 268
Effective length of database: 268
Effective search space:    71824
Effective search space used:    71824
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate WP_090272481.1 BLU11_RS05815 (methylisocitrate lyase)
to HMM TIGR02317 (prpB: methylisocitrate lyase (EC 4.1.3.30))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR02317.hmm
# target sequence database:        /tmp/gapView.2440633.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02317  [M=285]
Accession:   TIGR02317
Description: prpB: methylisocitrate lyase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.8e-127  410.4   0.5     2e-127  410.2   0.5    1.0  1  NCBI__GCF_900105005.1:WP_090272481.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900105005.1:WP_090272481.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  410.2   0.5    2e-127    2e-127       2     284 ..       6     290 ..       5     291 .. 0.99

  Alignments for each domain:
  == domain 1  score: 410.2 bits;  conditional E-value: 2e-127
                             TIGR02317   2 gkalrellkkedilqipGainalvallaekaGfeavYlsGaalaa.slglPDlglttleevaeearritrvtk 73 
                                           g+++r++l++e++lq++Gaina++alla++aGf+a+YlsG+++aa slglPDlg+ tle+v+ ++rrit+v++
  NCBI__GCF_900105005.1:WP_090272481.1   6 GQRFRQALAEEQPLQVIGAINANHALLAKRAGFKAIYLSGGGVAAgSLGLPDLGINTLEDVLIDVRRITDVCD 78 
                                           789****************************************999*************************** PP

                             TIGR02317  74 lpllvDaDtGfGe.alnvartvkeleeagvaavhieDqvapkkCGhldgkelvskeemvkkikaavkakkded 145
                                           lpl+vD+DtGfG  a+n++rtvk+l++ag+aa hieDqv +k+CGh++gke+vs+eemv++++aa++ak+d+d
  NCBI__GCF_900105005.1:WP_090272481.1  79 LPLMVDIDTGFGPsAFNIERTVKSLIKAGAAAAHIEDQVGAKRCGHRPGKEIVSTEEMVDRVRAAADAKTDPD 151
                                           ************769********************************************************** PP

                             TIGR02317 146 fvliaRtDaraveGldaaieRakaYveaGadaiftealeseeefrefakavkvpllanmtefGktplltadel 218
                                           f+liaRtDa   eG++aa+eR+k YveaGadaif+ea  ++  +++f k+++vp+lan+tefG tpl+t +el
  NCBI__GCF_900105005.1:WP_090272481.1 152 FFLIARTDAIQAEGVEAALERCKRYVEAGADAIFAEAAYDLPTYERFVKELNVPVLANITEFGATPLFTREEL 224
                                           ************************************************************************* PP

                             TIGR02317 219 eelgykiviyPvtalRaalkaaekvyeelkkkGtqkelldklqtRkelYellgyedyekkdkelfk 284
                                           +++g++i +yP++a+Raa+kaae+vy+ ++ +G q++++d++qtR+elY+ +gy+ +e k++ lf+
  NCBI__GCF_900105005.1:WP_090272481.1 225 ASVGVAIQLYPLSAFRAANKAAENVYNAVRTEGHQQNVIDSMQTREELYDRIGYHVFEGKLDALFA 290
                                           ************************************************************999985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (285 nodes)
Target sequences:                          1  (294 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 22.92
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources