GapMind for catabolism of small carbon sources

 

Protein WP_157682121.1 in Mucilaginibacter mallensis MP1X4

Annotation: NCBI__GCF_900105165.1:WP_157682121.1

Length: 527 amino acids

Source: GCF_900105165.1 in NCBI

Candidate for 19 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-fructose catabolism levG med PTFD aka LEVG, component of Fructose group translocator, LevDEFG (characterized) 42% 96% 212.6 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
sucrose catabolism levG med PTFD aka LEVG, component of Fructose group translocator, LevDEFG (characterized) 42% 96% 212.6 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
D-cellobiose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
D-glucosamine (chitosamine) catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
D-glucose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
lactose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
D-maltose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
D-mannose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
sucrose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
trehalose catabolism manZ lo PTS system mannose-specific EIID component; EII-M-Man; EIID-Man; Mannose permease IID component (characterized) 37% 93% 209.9 PTS mannose transporter subunit IID, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 50% 273.9
D-cellobiose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
D-glucose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
lactose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
D-maltose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
D-mannose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
sucrose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
trehalose catabolism manY lo PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 34% 98% 157.9 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
D-fructose catabolism levF lo PTFC aka LEVF, component of Fructose group translocator, LevDEFG (characterized) 33% 98% 142.1 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1
sucrose catabolism levF lo PTFC aka LEVF, component of Fructose group translocator, LevDEFG (characterized) 33% 98% 142.1 PTS sugar transporter, component of PTS uptake system for glucoselysine and fructoselysine, GfrABCD 45% 226.1

Sequence Analysis Tools

View WP_157682121.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSVSFILIALIAMFGHSEDFLGTTLLSRPLVLGPLVGLVLGDLNQGIIIGATLELIFMGN
IKVGAAIPPDVITGGVLGTAFAIISGKGPAIALAIAIPVSILAEMMISALFVLRAMLNKK
FNQYAEEGNYKKIQWLHILSGLIRPLLMGFIVLLALQLGANAMRSFLDMIPAWVQSGLQV
AGNMLPALGFALLMNLMFNKKVAPYFFLGFILASYLKLPIIAIGGIGVIIALIITQYMPV
NTNDDDDNNETQEPDGAPAVIHRLTNRDIRNIFFRSLALEANFNFETWQNTGFAFSIIPA
LKRIYTDKKQMAEALKRHLQLFNTSPYGSTLVLGITAAMEEQNSRDTDFDAESISSVKLG
LMGPLAGIFDSLFWGTFKVIAAGVGTSLALKGNIMGPVIFILIFNIPHLLLRYNLTFIGY
NAGTKFLSNLAKSNVMDKLTTGASILGLMVVGAMPATLMNITTPITIGSSKSAITIQGIL
DQIIPAIIPLGLTFLVYYFVKKGAKTAYLLLGLLVLGFVGSMIHLFA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory