Alignments for a candidate for fadA in Mucilaginibacter mallensis MP1X4

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadA in Mucilaginibacter mallensis MP1X4

Align 3-ketoacyl-CoA thiolase; Acetyl-CoA acyltransferase; Beta-ketothiolase; EC 2.3.1.16 (characterized)
to candidate WP_091380023.1 BLU33_RS24945 acetyl-CoA C-acyltransferase

Query= SwissProt::O32177
         (391 letters)



>NCBI__GCF_900105165.1:WP_091380023.1
          Length = 391

 Score =  389 bits (1000), Expect = e-113
 Identities = 200/388 (51%), Positives = 268/388 (69%), Gaps = 1/388 (0%)

Query: 4   AVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGN-IDDLIIGCATPEAE 62
           A IV+ +R+ VGKA +G     RPD L A  +K  +      + + I+D+I+G ATPEAE
Sbjct: 3   AYIVAASRSAVGKATRGGFRFTRPDTLAADVIKHLMASVPNVDKDEIEDVIVGNATPEAE 62

Query: 63  QGLNMARNIGALAGLPYTVPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESMSQ 122
           QGLN+AR I  ++     VP +TVNRYC+SGL++IA A+ KI  G  D  IAGG ESMS 
Sbjct: 63  QGLNVARLISLMSLDTDKVPGMTVNRYCASGLETIAIASAKIHAGIADCIIAGGVESMSL 122

Query: 123 VPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGVSREDQDAFAVRSHQNAAKALAE 182
           +PM G    PN  +A   P+YY  MG TAE VAK+Y ++R++QD FA  SHQ A  A+ E
Sbjct: 123 LPMGGWRIVPNADVALAHPDYYWGMGLTAEAVAKEYHINRDEQDLFAYNSHQKAISAIKE 182

Query: 183 GKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTVTA 242
           GKFKDEIVPV +T   + E  K  ++ F    DEG R  T+ D LS L+P F   G VTA
Sbjct: 183 GKFKDEIVPVNITEVYVDESGKKKKRDFKIDTDEGPRADTSIDALSKLKPVFDAKGVVTA 242

Query: 243 GNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALKLA 302
           GNSSQTSDGAA VM++         L P+ +  ++AV GVPP +MGIGP+ AIP+ LK+A
Sbjct: 243 GNSSQTSDGAAFVMVVSESFMKKNNLTPIARLVNYAVVGVPPRIMGIGPLYAIPKVLKMA 302

Query: 303 GLQLQDIGLFELNEAFASQAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSLIH 362
           G++ QD+ LFELNEAFASQ++ VI+ L ++ + +NVNGGAIALGHPLGC+G KL++ L +
Sbjct: 303 GMKQQDMDLFELNEAFASQSLAVIKGLDLNPDLINVNGGAIALGHPLGCSGAKLSVQLFN 362

Query: 363 EMKRRNEQFGVVTMCIGGGMGAAGVFEL 390
           E+K+R++++G+VTMC+G G GAAG+FE+
Sbjct: 363 ELKKRDQKYGMVTMCVGTGQGAAGIFEM 390


Lambda     K      H
   0.316    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 390
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 391
Length of database: 391
Length adjustment: 31
Effective length of query: 360
Effective length of database: 360
Effective search space:   129600
Effective search space used:   129600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate WP_091380023.1 BLU33_RS24945 (acetyl-CoA C-acyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01930.hmm
# target sequence database:        /tmp/gapView.3601041.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01930  [M=385]
Accession:   TIGR01930
Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
     7e-136  439.1   0.2   8.4e-136  438.8   0.2    1.1  1  NCBI__GCF_900105165.1:WP_091380023.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900105165.1:WP_091380023.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  438.8   0.2  8.4e-136  8.4e-136       1     385 []       5     389 ..       5     389 .. 0.95

  Alignments for each domain:
  == domain 1  score: 438.8 bits;  conditional E-value: 8.4e-136
                             TIGR01930   1 ivdavRtpig.klggslkelsaedLlaavikellera.gldpekidevilGnvlqageq.aniaReaalaagl 70 
                                           iv+a R+++g +++g ++ +++++L+a vik+l++ + ++d+++i++vi+Gn+++++eq  n+aR + l +  
  NCBI__GCF_900105165.1:WP_091380023.1   5 IVAASRSAVGkATRGGFRFTRPDTLAADVIKHLMASVpNVDKDEIEDVIVGNATPEAEQgLNVARLISLMSLD 77 
                                           8*********9889*********************************************************** PP

                             TIGR01930  71 pesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakledqllkd 143
                                            ++vp++tvnr+CaSgl+++a+a +ki+aG ad+++aGGvEsmS  p++  +        +++    +     
  NCBI__GCF_900105165.1:WP_091380023.1  78 TDKVPGMTVNRYCASGLETIAIASAKIHAGIADCIIAGGVESMSLLPMGGWRI------VPNADVALA----- 139
                                           ***********************************************966553......332211111..... PP

                             TIGR01930 144 lvktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk...........kkvv 205
                                            ++    mg tAe +ak+y+i+R+eqD +a++Shqka +Ai+egkfkdeivpv++ +            +  +
  NCBI__GCF_900105165.1:WP_091380023.1 140 HPDYYWGMGLTAEAVAKEYHINRDEQDLFAYNSHQKAISAIKEGKFKDEIVPVNITEVyvdesgkkkkrDFKI 212
                                           245679************************************************99879999**999988899 PP

                             TIGR01930 206 skDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivsaavagvd 278
                                           ++Deg+r++t++++L+kLkp+f+  kg +vtAgNssq++DGAa+++++se+++k+ +ltp+ar+v++av+gv+
  NCBI__GCF_900105165.1:WP_091380023.1 213 DTDEGPRADTSIDALSKLKPVFDA-KG-VVTAGNSSQTSDGAAFVMVVSESFMKKNNLTPIARLVNYAVVGVP 283
                                           **********************96.8*.7******************************************** PP

                             TIGR01930 279 peemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAiAlGHPlGasGar 351
                                           p++mg+gp +Ai+k+Lk ag++ +d+dl+E+nEAFA+q lav+k l+ l+++ +NvnGGAiAlGHPlG+sGa 
  NCBI__GCF_900105165.1:WP_091380023.1 284 PRIMGIGPLYAIPKVLKMAGMKQQDMDLFELNEAFASQSLAVIKGLD-LNPDLINVNGGAIALGHPLGCSGAK 355
                                           ***********************************************.88*********************** PP

                             TIGR01930 352 ivltllkeLkergkkyGlatlCvggGqGaAvile 385
                                           + ++l++eLk+r++kyG++t+Cvg+GqGaA i+e
  NCBI__GCF_900105165.1:WP_091380023.1 356 LSVQLFNELKKRDQKYGMVTMCVGTGQGAAGIFE 389
                                           *******************************997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (391 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 20.54
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources