Potential Gaps in catabolism of small carbon sources in Halobacillus alkaliphilus FP5
Found 82 low-confidence and 56 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 2-oxoglutarate | Psest_0085: 2-oxoglutarate TRAP transporter, solute receptor component | BMZ06_RS18735 | |
| 4-hydroxybenzoate | adh: acetaldehyde dehydrogenase (not acylating) | BMZ06_RS08010 | BMZ06_RS19550 |
| 4-hydroxybenzoate | mhpD: 2-hydroxypentadienoate hydratase | | |
| 4-hydroxybenzoate | mhpE: 4-hydroxy-2-oxovalerate aldolase | | |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| 4-hydroxybenzoate | xylF: 2-hydroxymuconate semialdehyde hydrolase | | |
| acetate | actP: cation/acetate symporter ActP | BMZ06_RS11800 | |
| arabinose | araE: L-arabinose:H+ symporter | BMZ06_RS14425 | |
| arabinose | KDG-aldolase: 2-dehydro-3-deoxy-L-arabinonate aldolase | | |
| arabinose | xacB: L-arabinose 1-dehydrogenase | BMZ06_RS09060 | BMZ06_RS02025 |
| arabinose | xacC: L-arabinono-1,4-lactonase | BMZ06_RS14615 | |
| arabinose | xacD: L-arabinonate dehydratase | BMZ06_RS09425 | BMZ06_RS07445 |
| arginine | rocE: L-arginine permease | BMZ06_RS01185 | |
| asparagine | ans: asparaginase | BMZ06_RS03390 | BMZ06_RS17170 |
| citrate | tctB: citrate/Na+ symporter, small transmembrane component TctB | | |
| citrate | tctC: citrate/Na+ symporter, substrate-binding component TctC | BMZ06_RS18850 | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | BMZ06_RS15960 | BMZ06_RS19810 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | BMZ06_RS19805 | BMZ06_RS15955 |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | BMZ06_RS19805 | BMZ06_RS15955 |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | | |
| citrulline | arcC: carbamate kinase | | |
| D-alanine | cycA: D-alanine:H+ symporter CycA | | |
| D-alanine | dadA: D-alanine dehydrogenase | | |
| D-serine | cycA: D-serine:H+ symporter CycA | | |
| deoxyinosine | adh: acetaldehyde dehydrogenase (not acylating) | BMZ06_RS08010 | BMZ06_RS19550 |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | BMZ06_RS08630 | BMZ06_RS08530 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | adh: acetaldehyde dehydrogenase (not acylating) | BMZ06_RS08010 | BMZ06_RS19550 |
| deoxyribose | deoP: deoxyribose transporter | | |
| ethanol | adh: acetaldehyde dehydrogenase (not acylating) | BMZ06_RS08010 | BMZ06_RS19550 |
| fucose | fucA: L-fuculose-phosphate aldolase FucA | | |
| fucose | fucI: L-fucose isomerase FucI | | |
| fucose | fucK: L-fuculose kinase FucK | | |
| fucose | fucP: L-fucose:H+ symporter FucP | | |
| fucose | fucU: L-fucose mutarotase FucU | | |
| galactose | galP: galactose:H+ symporter GalP | BMZ06_RS14425 | |
| galacturonate | exuT: D-galacturonate transporter ExuT | | |
| galacturonate | kdgK: 2-keto-3-deoxygluconate kinase | BMZ06_RS08150 | BMZ06_RS20040 |
| galacturonate | uxaA: D-altronate dehydratase | | |
| galacturonate | uxaB: tagaturonate reductase | | |
| galacturonate | uxaC: D-galacturonate isomerase | | |
| glucosamine | gamP: glucosamine PTS system, EII-CBA components (GamP/NagE) | BMZ06_RS15350 | BMZ06_RS08640 |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | | |
| glucuronate | gci: D-glucaro-1,4-lactone cycloisomerase | BMZ06_RS07445 | |
| glucuronate | kdgD: 5-dehydro-4-deoxyglucarate dehydratase | | |
| glucuronate | udh: D-glucuronate dehydrogenase | | |
| histidine | hutG: N-formiminoglutamate formiminohydrolase | BMZ06_RS06660 | |
| histidine | hutH: histidine ammonia-lyase | | |
| histidine | hutI: imidazole-5-propionate hydrolase | | |
| histidine | hutU: urocanase | | |
| histidine | PA5503: L-histidine ABC transporter, ATPase component | BMZ06_RS17230 | BMZ06_RS09230 |
| histidine | PA5504: L-histidine ABC transporter, permease component | BMZ06_RS17225 | BMZ06_RS01405 |
| histidine | PA5505: L-histidine ABC transporter, substrate-binding component | BMZ06_RS09220 | BMZ06_RS17220 |
| isoleucine | acdH: (2S)-2-methylbutanoyl-CoA dehydrogenase | BMZ06_RS06705 | BMZ06_RS02695 |
| isoleucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | BMZ06_RS06115 | BMZ06_RS01115 |
| isoleucine | brnQ: L-isoleucine:cation symporter BrnQ/BraZ/BraB | BMZ06_RS13040 | |
| isoleucine | ivdG: 3-hydroxy-2-methylbutyryl-CoA dehydrogenase | BMZ06_RS05280 | BMZ06_RS07330 |
| isoleucine | pccA: propionyl-CoA carboxylase, alpha subunit | BMZ06_RS00985 | BMZ06_RS02705 |
| lactose | lacE: lactose ABC transporter, substrate-binding component | BMZ06_RS16905 | |
| lactose | lacK: lactose ABC transporter, ATPase component | BMZ06_RS19030 | BMZ06_RS13115 |
| leucine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | BMZ06_RS06115 | BMZ06_RS01115 |
| leucine | brnQ: L-leucine:Na+ symporter BrnQ/BraB | BMZ06_RS13040 | |
| leucine | liuA: isovaleryl-CoA dehydrogenase | BMZ06_RS02695 | BMZ06_RS06710 |
| leucine | liuB: 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit | BMZ06_RS10120 | BMZ06_RS02705 |
| leucine | liuD: 3-methylcrotonyl-CoA carboxylase, beta subunit | BMZ06_RS02725 | BMZ06_RS01160 |
| lysine | davA: 5-aminovaleramidase | BMZ06_RS19530 | |
| lysine | davB: L-lysine 2-monooxygenase | | |
| lysine | davD: glutarate semialdehyde dehydrogenase | BMZ06_RS06000 | BMZ06_RS09860 |
| lysine | lysP: L-lysine:H+ symporter LysP | | |
| myoinositol | iolB: 5-deoxy-D-glucuronate isomerase | | |
| myoinositol | iolC: 5-dehydro-2-deoxy-D-gluconate kinase | BMZ06_RS20040 | |
| myoinositol | iolD: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase | | |
| myoinositol | iolE: scyllo-inosose 2-dehydratase | | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | BMZ06_RS07110 | BMZ06_RS15080 |
| myoinositol | iolJ: 5-dehydro-2-deoxyphosphogluconate aldolase | BMZ06_RS06750 | BMZ06_RS20040 |
| myoinositol | iolT: myo-inositol:H+ symporter | BMZ06_RS14425 | |
| phenylacetate | paaA: phenylacetyl-CoA 1,2-epoxidase, subunit A | | |
| phenylacetate | paaB: phenylacetyl-CoA 1,2-epoxidase, subunit B | | |
| phenylacetate | paaC: phenylacetyl-CoA 1,2-epoxidase, subunit C | | |
| phenylacetate | paaE: phenylacetyl-CoA 1,2-epoxidase, subunit E | | |
| phenylacetate | paaF: 2,3-dehydroadipyl-CoA hydratase | BMZ06_RS02720 | BMZ06_RS18940 |
| phenylacetate | paaG: 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase | BMZ06_RS18055 | BMZ06_RS02720 |
| phenylacetate | paaJ1: 3-oxo-5,6-dehydrosuberyl-CoA thiolase | BMZ06_RS07305 | BMZ06_RS07240 |
| phenylacetate | paaJ2: 3-oxoadipyl-CoA thiolase | BMZ06_RS07240 | BMZ06_RS07305 |
| phenylacetate | paaK: phenylacetate-CoA ligase | BMZ06_RS18930 | BMZ06_RS07250 |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylacetate | paaZ1: oxepin-CoA hydrolase | BMZ06_RS18055 | |
| phenylacetate | paaZ2: 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase | | |
| phenylalanine | aroP: L-phenylalanine:H+ symporter AroP | BMZ06_RS01185 | |
| phenylalanine | fahA: fumarylacetoacetate hydrolase | BMZ06_RS04555 | BMZ06_RS06095 |
| phenylalanine | hmgA: homogentisate dioxygenase | | |
| phenylalanine | HPD: 4-hydroxyphenylpyruvate dioxygenase | | |
| phenylalanine | maiA: maleylacetoacetate isomerase | | |
| phenylalanine | PAH: phenylalanine 4-monooxygenase | | |
| phenylalanine | PCBD: pterin-4-alpha-carbinoalamine dehydratase | BMZ06_RS09795 | |
| phenylalanine | QDPR: 6,7-dihydropteridine reductase | BMZ06_RS04250 | |
| propionate | pccA: propionyl-CoA carboxylase, alpha subunit | BMZ06_RS00985 | BMZ06_RS02705 |
| propionate | prpE: propionyl-CoA synthetase | BMZ06_RS09710 | BMZ06_RS02730 |
| putrescine | gabT: gamma-aminobutyrate transaminase | BMZ06_RS06005 | BMZ06_RS09875 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | BMZ06_RS10380 | BMZ06_RS18675 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | BMZ06_RS07645 | BMZ06_RS18260 |
| putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
| pyruvate | yjcH: putative small subunit of pyruvate transporter (yjcH-like) | | |
| rhamnose | LRA1: L-rhamnofuranose dehydrogenase | BMZ06_RS05280 | BMZ06_RS13185 |
| rhamnose | LRA2: L-rhamnono-gamma-lactonase | | |
| rhamnose | LRA3: L-rhamnonate dehydratase | BMZ06_RS07445 | |
| rhamnose | LRA4: 2-keto-3-deoxy-L-rhamnonate aldolase | | |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsA: D-ribose ABC transporter, ATPase component RbsA | BMZ06_RS04075 | BMZ06_RS05745 |
| ribose | rbsB: D-ribose ABC transporter, substrate-binding component RbsB | BMZ06_RS04065 | |
| ribose | rbsC: D-ribose ABC transporter, permease component RbsC | BMZ06_RS04070 | |
| serine | serP: L-serine permease SerP | | |
| sorbitol | sdh: sorbitol dehydrogenase | BMZ06_RS07160 | BMZ06_RS07165 |
| sorbitol | SOT: sorbitol:H+ co-transporter SOT1 or SOT2 | BMZ06_RS14425 | |
| threonine | adh: acetaldehyde dehydrogenase (not acylating) | BMZ06_RS08010 | BMZ06_RS19550 |
| threonine | gcvP: glycine cleavage system, P component (glycine decarboxylase) | BMZ06_RS00885 | BMZ06_RS00880 |
| threonine | tdcC: L-threonine:H+ symporter TdcC | | |
| thymidine | adh: acetaldehyde dehydrogenase (not acylating) | BMZ06_RS08010 | BMZ06_RS19550 |
| trehalose | treC: trehalose-6-phosphate hydrolase | BMZ06_RS07930 | BMZ06_RS18880 |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | BMZ06_RS01185 | |
| tyrosine | fahA: fumarylacetoacetate hydrolase | BMZ06_RS04555 | BMZ06_RS06095 |
| tyrosine | hmgA: homogentisate dioxygenase | | |
| tyrosine | HPD: 4-hydroxyphenylpyruvate dioxygenase | | |
| tyrosine | maiA: maleylacetoacetate isomerase | | |
| valine | acdH: isobutyryl-CoA dehydrogenase | BMZ06_RS06705 | BMZ06_RS02695 |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | BMZ06_RS02720 | |
| valine | bkdC: branched-chain alpha-ketoacid dehydrogenase, E2 component | BMZ06_RS06115 | BMZ06_RS01115 |
| valine | brnQ: L-valine:cation symporter BrnQ/BraZ/BraB | BMZ06_RS13040 | |
| valine | ech: (S)-3-hydroxybutanoyl-CoA hydro-lyase | BMZ06_RS02720 | BMZ06_RS18940 |
| valine | mmsB: 3-hydroxyisobutyrate dehydrogenase | | |
| valine | pccA: propionyl-CoA carboxylase, alpha subunit | BMZ06_RS00985 | BMZ06_RS02705 |
| xylitol | fruI: xylitol PTS, enzyme IIABC (FruI) | BMZ06_RS09525 | |
| xylose | xylT: D-xylose transporter | BMZ06_RS14425 | |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Links
Downloads
Related tools
About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory