Alignments for a candidate for prpE in Halobacillus alkaliphilus FP5

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Halobacillus alkaliphilus FP5

Align Acyl-coenzyme A synthetase ACSM3, mitochondrial; Acyl-CoA synthetase medium-chain family member 3; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; Middle-chain acyl-CoA synthetase 3; Propionate--CoA ligase; Protein SA homolog; EC 6.2.1.2; EC 6.2.1.17 (characterized)
to candidate WP_089751845.1 BMZ06_RS09710 acyl--CoA ligase

Query= SwissProt::Q3UNX5
         (580 letters)



>NCBI__GCF_900113125.1:WP_089751845.1
          Length = 524

 Score =  384 bits (985), Expect = e-111
 Identities = 211/529 (39%), Positives = 304/529 (57%), Gaps = 18/529 (3%)

Query: 49  PEYFNFAKDVLDQWTNMEKAGKRLSNPAFWWIDGNGEELRWSFEELGLLSRKFANILTEA 108
           PE +N   +       MEK  +     A  W+   G     ++E+L   + K  N   + 
Sbjct: 9   PERYNVVSE-------MEKFAEDPDRKALLWLSEAGGRKEVTYEQLLHQANKMGNAFLDH 61

Query: 109 CSLQRGDRVMVILPKIPEWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAKCIITD 168
             LQ+GD+++V++P++ E +   +A L+ G V+IP +  L  KD+ YR+     + +I+ 
Sbjct: 62  -GLQKGDKILVMIPRLIEAYQVYIAALKAGLVIIPSSEMLRAKDLQYRITHGDVRAVISY 120

Query: 169 DTLAPAVDAVAAKCENLHSKLIVSQHSREGWGNLKEMMKYASDSHTCVDTKHDEMMAIYF 228
                 +D V    + L   +     S+EGW +   +++  S +    DT  D+M  + +
Sbjct: 121 HPFTEHLDEVKESDQLLKFSV---GGSKEGWFDFDGLLEDYSSTLETADTASDDMAFLSY 177

Query: 229 TSGTTGPPKMIGHTHSSFGLGLSVNGRFWLDLIASDVMWNTSDTGWAKSAWSSVFSPWTQ 288
           TSGTTG PK + HTH      L      WL +   D +W T+  GW K  WS   S    
Sbjct: 178 TSGTTGNPKGVVHTHGWGYAHLKTAAYKWLAIQDGDTVWATAGPGWQKWVWSPFLSVLGT 237

Query: 289 GACVFAHYLPRFESTSILQTLSKFPITVFCSAPTAYRMLVQND-MSSYKFNSLKHCVSAG 347
           GA     Y  +F   + L  + +  + V C  PT YR++ + D +  Y+   L   VSAG
Sbjct: 238 GARGLV-YQGKFNPKTYLSLMDEHNVNVLCCTPTEYRLMAKVDNLDDYQLAGLHSAVSAG 296

Query: 348 EPINPEVMEQWRKKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKILDEN 407
           EP+N EV++ ++    + + +GYGQTE  L+ G  K M+++PGSMG+P+P   V+I+DE 
Sbjct: 297 EPLNREVIDTFKNHFNVTVRDGYGQTENTLLVGILKDMEVRPGSMGRPTPGNQVEIIDEL 356

Query: 408 GATLPPGQEGDIALQVLPERPFGLFTHYVDNPSKTASTLRGSFYITGDRGYMDEDGYFWF 467
              +  G+ GDIA+ +  + P  LF  Y  +P +T  + RG +Y+TGD+   DEDGYFWF
Sbjct: 357 AEPVETGEVGDIAVHL--DTP-ALFKEYYKDPERTKMSHRGHYYVTGDQARKDEDGYFWF 413

Query: 468 VARSDDIILSSGYRIGPFEVESALIEHPSIAESAVVSSPDPIRGEVVKAFIVLNPDYKSH 527
             RSDDII+SSGY IGPFEVE AL++H ++ E AVVSSPD +RG VVKAFIVL  +Y+  
Sbjct: 414 EGRSDDIIISSGYTIGPFEVEDALVKHKAVQECAVVSSPDEVRGNVVKAFIVLQNNYEPT 473

Query: 528 DQEQLKKEIQEHVKKTTAPYKYPRKVEFIEELPKTVSGKVKRNELRKKE 576
           D   L KE+Q+HVK TTAPYKYPRK+EF EELPKT SGK++R ELRKKE
Sbjct: 474 D--SLVKELQDHVKNTTAPYKYPRKIEFTEELPKTTSGKIRRVELRKKE 520


Lambda     K      H
   0.319    0.134    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 816
Number of extensions: 44
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 580
Length of database: 524
Length adjustment: 36
Effective length of query: 544
Effective length of database: 488
Effective search space:   265472
Effective search space used:   265472
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources