GapMind for catabolism of small carbon sources

 

Protein WP_076476644.1 in Williamsia sterculiae CPCC 203464

Annotation: NCBI__GCF_900156495.1:WP_076476644.1

Length: 480 amino acids

Source: GCF_900156495.1 in NCBI

Candidate for 22 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-phenylalanine catabolism pad-dh med phenylacetaldehyde dehydrogenase (EC 1.2.1.39) (characterized) 44% 99% 417.2 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
4-hydroxybenzoate catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
2'-deoxyinosine catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
2-deoxy-D-ribose catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
ethanol catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-threonine catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
thymidine catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-tryptophan catabolism adh med Putative aldehyde dehydrogenase FUS7; Fusarin biosynthesis protein 7; EC 1.2.1.3 (characterized) 40% 99% 362.5 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-arginine catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 37% 97% 299.3 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-citrulline catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 37% 97% 299.3 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-lysine catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 37% 97% 299.3 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-proline catabolism davD lo Glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 37% 97% 299.3 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-arginine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 34% 98% 280.4 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-citrulline catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 34% 98% 280.4 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-lysine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 34% 98% 280.4 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
putrescine catabolism patD lo aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) 34% 98% 280.4 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
4-hydroxybenzoate catabolism praB lo 2-aminomuconate semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 35% 98% 280 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-tryptophan catabolism nbaE lo 2-aminomuconate semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 35% 98% 280 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-tryptophan catabolism praB lo 2-aminomuconate semialdehyde dehydrogenase (EC 1.2.1.32) (characterized) 35% 98% 280 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-fucose catabolism aldA lo NAD+-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 36% 100% 277.7 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-rhamnose catabolism aldA lo NAD+-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 36% 100% 277.7 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9
L-threonine catabolism aldA lo NAD+-dependent L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 36% 100% 277.7 aromatic aldehyde dehydrogenase (EC 1.2.1.67; EC 1.2.1.29) 49% 427.9

Sequence Analysis Tools

View WP_076476644.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSTPTDVRYANVINGETRSSAESAPVYNPATGQVFAEVPVATRADLDDAVTAAAAAYPGW
SATSAAERAAAVSAIGDRLEAHAEEFITLLTAEQGKPRSMAEWEVYGSVAWFREIAKQQL
PDEVVEDTPERRVISRYTPLGVVGAIVPWNFPILLAVWKIAPALVTGNTIIVKPSPFTPL
CDLKLVELVQDLLPPGVLSALSGDDDLGKWMTVHPGIAKIAFTGSTETGRHVMASAAGTL
KRVTLELGGNDPAIVLRDVDPQKVAPQIFWAAFQNNAQFCNAAKRIYIHDDVYDAVRDEL
VEYARSVVVGDGSHPDTQLGPIQNRPQLAKVTEYFDDCRKNGYTFALGGEIDTESEGWFV
PVTLVDNPPEDSRLVAEEPFGPILPLLKWSEEDDVVARANDTVWGLGATVWGDDLEAVER
IGRRIEAGTVWLNEVHQYSPHQVFGGHKQSGIGAENSLHGLAEYTNYQTVCLNRAPGTVA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory