Alignments for a candidate for iolA in Williamsia sterculiae CPCC 203464

GapMind for catabolism of small carbon sources

Alignments for a candidate for iolA in Williamsia sterculiae CPCC 203464

Align malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)
to candidate WP_076481165.1 BW971_RS14520 CoA-acylating methylmalonate-semialdehyde dehydrogenase

Query= reanno::Smeli:SMc00781
         (498 letters)



>NCBI__GCF_900156495.1:WP_076481165.1
          Length = 520

 Score =  681 bits (1756), Expect = 0.0
 Identities = 335/516 (64%), Positives = 402/516 (77%), Gaps = 21/516 (4%)

Query: 3   ELGHFIDGKRVAGTSG----RVSNIFNPATGEVQGTVALASDADLAAAVESAKAAQPKWA 58
           ++ H ++G+R    +G    R + + NP+TG V+G VA+A+ AD+ AAV SA  AQ  WA
Sbjct: 4   QIPHLVNGERTMSPAGDTTPRTAEVLNPSTGAVRGQVAMATAADVDAAVASAAVAQRAWA 63

Query: 59  ATNPQRRARVFMKFVQLLNDNMNELAEMLSREHGKTIDDAKGDIVRGLEVCEFVIGIPHL 118
           A NPQRRARV M+FV L+N N +ELAE+LS EHGKT+ DA+GDI RG+EV EF IG PHL
Sbjct: 64  AWNPQRRARVMMRFVDLVNQNADELAELLSVEHGKTVADARGDIQRGVEVIEFAIGAPHL 123

Query: 119 QKSEFTEGAGPGIDMYSIRQPVGIGAGITPFNFPGMIPMWMFAPAIACGNAFILKPSERD 178
            K E+T GAG GID+YS+RQP+G+ AGITPFNFP MIP+W   PA+ACGNAFILKPSERD
Sbjct: 124 LKGEYTVGAGAGIDVYSVRQPLGVVAGITPFNFPAMIPLWKAGPALACGNAFILKPSERD 183

Query: 179 PSVPIRLAELMIEAGLPAGILNVVNGDKGAVDAILTHPDIAAVSFVGSTPIARYVYGTAA 238
           PSVP+RLAEL +EAGLPAG   V+ GDK AVDA+L +PD+AA+ FVGS+ IA+Y+Y TA 
Sbjct: 184 PSVPLRLAELFVEAGLPAGAFQVIQGDKEAVDALLANPDVAAIGFVGSSDIAQYIYATAT 243

Query: 239 MNGKRAQCFGGAKNHMIIMPDADLDQAANALIGAGYGSAGERCMAISVAVPVGEETANRL 298
            NGKRAQCFGGAKNHMI+MPDADLDQA +ALIGAGYGSAGERCMAISVAVPVGEETA+RL
Sbjct: 244 ANGKRAQCFGGAKNHMIVMPDADLDQAVDALIGAGYGSAGERCMAISVAVPVGEETADRL 303

Query: 299 IDKLVPMVESLRIGPYTDEKADMGPVVTKEAEQRIRSLIDSGIEQGAKLVVDGRD----- 353
            ++LV  +  LR+G   D KAD GP++T  A +R+R  + +G+E GA++VVDGR+     
Sbjct: 304 RERLVDKIAGLRVGHSLDPKADYGPLITPAALERVRGYLAAGVEAGAEMVVDGRERASDD 363

Query: 354 --FKLQGYENGHFIGGCLFDDVTPDMDIYKTEIFGPVLSVVRARNYEEALSLPMKHEYGN 411
             F       G+FIG  LFD VTPDM IY  EIFGPVL +VRA +YEEAL+LP +HEYGN
Sbjct: 364 LQFGDDSLAGGNFIGPTLFDHVTPDMSIYTDEIFGPVLCIVRAADYEEALALPSEHEYGN 423

Query: 412 GVAIYTRDGDAARDFASRINIGMVGVNVPIPVPLAYHSFGGWKSSSFGDLNQHGTDSIKF 471
           GVAI+TRDGDAARDF  R+ +GMVGVNVPIPVP+AYH+FGGWK S FGDLNQHG  S +F
Sbjct: 424 GVAIFTRDGDAARDFTERVQVGMVGVNVPIPVPVAYHTFGGWKRSGFGDLNQHGPASFQF 483

Query: 472 WTRTKTITSRWPSGIKDG----------AEFSIPTM 497
           +T+ KTITSRWPSGIKDG          AEFSIPTM
Sbjct: 484 YTKVKTITSRWPSGIKDGASGATGYDGSAEFSIPTM 519


Lambda     K      H
   0.319    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 865
Number of extensions: 25
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 498
Length of database: 520
Length adjustment: 34
Effective length of query: 464
Effective length of database: 486
Effective search space:   225504
Effective search space used:   225504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources