Alignments for a candidate for pccB in Williamsia sterculiae CPCC 203464

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccB in Williamsia sterculiae CPCC 203464

Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate WP_076480878.1 BW971_RS14630 acetyl-CoA carboxylase carboxyltransferase subunit

Query= metacyc::MONOMER-17283
         (535 letters)



>NCBI__GCF_900156495.1:WP_076480878.1
          Length = 532

 Score =  477 bits (1227), Expect = e-139
 Identities = 260/543 (47%), Positives = 346/543 (63%), Gaps = 19/543 (3%)

Query: 1   MSIIHSHIQPNSPDFQANFAYHQSLAADLRERLAQIRQGGGAEQRRRHEERGKLFVRDRI 60
           M+ + S + P    + A     ++   +L+    +   GGG ++ +RH  RGK+  R+RI
Sbjct: 1   MTALRSTLDPTGEGYAAAATSMEAKLIELQVEFDKALAGGGEKKVQRHHSRGKMLARERI 60

Query: 61  DTLIDPDSSFLEIGALAAYNVYDEEVPAAGIVCGIGRVAGRPVMIIANDATVKGGTYFPL 120
           + L+DPD++FLE+  LA Y   D +V  A +V GIG V G   MI+AND TV+GGT  P 
Sbjct: 61  ELLVDPDTAFLELCPLAGYGS-DFQV-GASLVVGIGVVEGVECMIVANDPTVRGGTSNPW 118

Query: 121 TVKKHLRAQEIARENRLPCIYLVDSGGAYLPLQSEVFPDRDHFGRIFYNQAQMSAEGIPQ 180
           T+KK  RA EIA +NRLPCI LV+SGGA LP Q EVF      GR+F +  Q+SA GIP 
Sbjct: 119 TLKKGFRANEIALQNRLPCISLVESGGADLPTQKEVFIPG---GRMFRDITQLSAAGIPT 175

Query: 181 IACVMGSCTAGGAYVPAMSDEVVIVKGNGTIFLGGPPLVKAATGEEVTAEELGGADVHTR 240
           IA V G+ TAGGAY+P MSD  V+++    +FLGGPPLVK ATGEE   E LGGA +H R
Sbjct: 176 IALVFGNSTAGGAYIPGMSDHTVMIENRSKVFLGGPPLVKMATGEESDDESLGGAQMHAR 235

Query: 241 ISGVADYFANDDREALAIVRDIVAHLGPRQRANWELRDPEP------PRYDPREIYGILP 294
            SG+ADY+A D+++A+ I R IVA      R NW  + P P      PRYDP E+ GI+P
Sbjct: 236 QSGLADYYAQDEQDAVRIGRRIVA------RLNWHKKGPGPIAPSVEPRYDPEELLGIVP 289

Query: 295 RDFRQSYDVREVIARIVDGSRLHEFKTRYGTTLVCGFAHIEGFPVGILAN-NGILFSESA 353
            D +  +D R+VIAR+VD S   EFK+ YG++LV G+A I G+PVGILAN  G+LFS+ A
Sbjct: 290 ADLKIPFDPRDVIARVVDDSDFDEFKSEYGSSLVTGWARIHGYPVGILANAQGVLFSQEA 349

Query: 354 LKGAHFIELCCARNIPLVFLQNITGFMVGKQYENGGIAKDGAKLVTAVSCANVPKFTVII 413
            K   FI+L    + PL+FL N TG+MVGK+YE  GI K G+ ++ AVS + VP  ++++
Sbjct: 350 QKATQFIQLANRSDTPLLFLHNTTGYMVGKEYEQNGIIKHGSMMINAVSNSTVPHISLLV 409

Query: 414 GGSFGAGNYGMCGRAYQPRQLWMWPNARISVMGGTQAANVLLTIRRDNLRARGQDMTPEE 473
           G S+GAG+YGMCGRAY PR L+ WP+A+ +VMG  Q A V+  + R    ARG  +    
Sbjct: 410 GASYGAGHYGMCGRAYDPRFLFAWPSAKSAVMGAAQLAGVISLVSRAATEARGGTVDEAA 469

Query: 474 QERFMAPILAKYEQEGHPYYASARLWDDGVIDPVETRRVLALGLAAAAEAPVQPT-RFGV 532
                  I A+ E+E  P + S  L+DDG+IDP +TR VL L L+     PV+ T  FGV
Sbjct: 470 DAGMKQMIEAQIEKESVPAFLSGMLYDDGIIDPRDTRTVLGLCLSTIDNTPVEGTSNFGV 529

Query: 533 FRM 535
           FRM
Sbjct: 530 FRM 532


Lambda     K      H
   0.322    0.139    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 801
Number of extensions: 35
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 535
Length of database: 532
Length adjustment: 35
Effective length of query: 500
Effective length of database: 497
Effective search space:   248500
Effective search space used:   248500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources