Alignments for a candidate for mcmA in Williamsia sterculiae CPCC 203464

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcmA in Williamsia sterculiae CPCC 203464

Align methylmalonyl-CoA mutase (EC 5.4.99.2) (characterized)
to candidate WP_076477891.1 BW971_RS07055 methylmalonyl-CoA mutase

Query= BRENDA::Q84FZ1
         (721 letters)



>NCBI__GCF_900156495.1:WP_076477891.1
          Length = 759

 Score = 1006 bits (2601), Expect = 0.0
 Identities = 512/708 (72%), Positives = 591/708 (83%), Gaps = 13/708 (1%)

Query: 19  KAPPPPAGEPWMTPEGIPVKGFYGPEDREGCEG-------IDSFPGLPPYLRGPYPAMYV 71
           + P P A   W TPE I V   +   DR+           +DS PG  P+LRGPYP MYV
Sbjct: 35  RTPRPEAQTLWTTPEQIDVPPVFTRADRDAVATSGEHPYPLDSVPGAVPFLRGPYPTMYV 94

Query: 72  TQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAG 131
            QPWTIRQYAGFSTA +SNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRV+GDVGMAG
Sbjct: 95  NQPWTIRQYAGFSTAAESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVAGDVGMAG 154

Query: 132 VAIDSIYDMRTLFSGIPLDEMTVSMTMNGAVLPGVALYIVAAEEQGVPPEKLAGTIQNDI 191
           VAIDSI DMR LF GI L  ++VSMTMNGAVLP +ALY+VAAEEQGVP EKLAGTIQNDI
Sbjct: 155 VAIDSILDMRQLFDGIDLGSVSVSMTMNGAVLPILALYVVAAEEQGVPAEKLAGTIQNDI 214

Query: 192 LKEFMVRNTYIYPPKGSMRIISDIFGYTSKNMPKFNSISISGYHMQEAGATQDLELAYTL 251
           LKEFMVRNTYIYPP+ SMRIISDIF +TS  MP+FNSISISGYH+QEAGAT DLELAYTL
Sbjct: 215 LKEFMVRNTYIYPPEPSMRIISDIFKFTSTEMPRFNSISISGYHIQEAGATADLELAYTL 274

Query: 252 ADGVEYIKAGLAAGLTIDQFAPRLSFFWAIGMNFFMEIAKMRAARLIWAKLVKEFEPKSD 311
           ADGVEYI+AGLAAGL +D+FAPRLSFFW IGMNFFME+AK+RAARL+W++LV +FEP++ 
Sbjct: 275 ADGVEYIRAGLAAGLDVDKFAPRLSFFWGIGMNFFMEVAKLRAARLLWSELVAQFEPENP 334

Query: 312 KSLPLRTHSQTSGWSLTAQDVFNNVTRTCIEAMAATQGGTQSLHTNALDEALALPTDFSA 371
           KSL LRTHSQTSGWSLTAQD FNNV RTCIEAMAATQG TQSLHTNALDEALALPTDFSA
Sbjct: 335 KSLSLRTHSQTSGWSLTAQDPFNNVARTCIEAMAATQGHTQSLHTNALDEALALPTDFSA 394

Query: 372 RIARNTQLFLQQESGTTRIIDPWGGSYYVERLTRDIAARAWEHIREVEALGGMAKAIEAG 431
           RIARNTQL LQQESGTTR IDPWGGS YVE LT  +A RA  HI EVE  GGMA+AI  G
Sbjct: 395 RIARNTQLLLQQESGTTRPIDPWGGSNYVEWLTAQLADRARAHIAEVEEAGGMAQAINEG 454

Query: 432 IPKLRIEEAAARAQARIDSGRQTIVGINKYKPDDEMKIDLLRVDNADVRAKQIDKLKRLR 491
           +PKLRIEEAAAR QARIDSG+QT++GIN+Y+  ++ +ID+L+V+N+ VRA+Q+DKL RLR
Sbjct: 455 LPKLRIEEAAARTQARIDSGQQTVIGINRYQVTEDQEIDVLKVENSKVRAEQLDKLDRLR 514

Query: 492 AERNQADVDAALAALTKAADG-----EGNLLELAVNAARAKATVGEISEALEKAWGRHRA 546
           AER++A V  AL  L++AA       E NLL LA+ AAR +ATVGEIS ALEK +GRH+A
Sbjct: 515 AERDEAAVSRALDDLSRAAGDTSGGRENNLLALAIEAARHQATVGEISTALEKVYGRHQA 574

Query: 547 QIRSISGVYKREVGGMSPVVEKVRGLVEAFEENDGRRPRILVAKMGQDGHDRGQKVIASA 606
           +I++ISGVY+ E G +S + + +   VE F + +GRRPR+L+AKMGQDGHDRGQKVIA+A
Sbjct: 575 EIKTISGVYRHEAGSVSNIDDAI-AAVERFADAEGRRPRVLIAKMGQDGHDRGQKVIATA 633

Query: 607 FADLGFDVDIGPLFATPDEAARQAVENDVHIVGVSSLAAGHLTLVPELKAALKQEGRDDV 666
           FADLGFDVD+GPLF TPDE ARQA +NDVH+VGVSSLAAGHLTLVP L+ AL + GR D+
Sbjct: 634 FADLGFDVDVGPLFQTPDEVARQAADNDVHVVGVSSLAAGHLTLVPALRNALAEVGRPDI 693

Query: 667 MIVVGGVIPPGDYDALYAAGASAIFPPGTVIAEAAVNLLGELNTRLGY 714
           MIVVGGVIPPGD+D LY AGA+AIFPPGTVIA++A+ L+ +L + LG+
Sbjct: 694 MIVVGGVIPPGDFDELYQAGAAAIFPPGTVIADSALELIDKLGSALGH 741


Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1436
Number of extensions: 49
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 721
Length of database: 759
Length adjustment: 40
Effective length of query: 681
Effective length of database: 719
Effective search space:   489639
Effective search space used:   489639
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources