Alignments for a candidate for arcB in Sphingomonas histidinilytica UM2

GapMind for catabolism of small carbon sources

Alignments for a candidate for arcB in Sphingomonas histidinilytica UM2

Align ornithine carbamoyltransferase (EC 2.1.3.3) (characterized)
to candidate WP_079647873.1 B5X82_RS09400 ornithine carbamoyltransferase

Query= BRENDA::Q98BB6
         (303 letters)



>NCBI__GCF_900167915.1:WP_079647873.1
          Length = 309

 Score =  338 bits (867), Expect = 9e-98
 Identities = 173/305 (56%), Positives = 211/305 (69%), Gaps = 7/305 (2%)

Query: 4   RHFTDLSTVSEGDLRFMLDDAVVRKARLKAG------ERTRPLEGKVLAMIFDKPSTRTR 57
           R F DL+      +R MLD+AV RK + +AG      +R  PL G  LAMIF+K STRTR
Sbjct: 3   RSFLDLADAGAAGVRLMLDEAVARK-KARAGLPKGAADRDAPLAGHTLAMIFEKNSTRTR 61

Query: 58  VSFDVGMRQLGGETIMLTGTEMQLGRSETIADTAKVLSRYVDAIMIRTTSHDRLLELTEN 117
           VSFD+ MRQLGG TI++    MQLGR ETIADTA+VLSRYVDAIMIRT  H + ++L + 
Sbjct: 62  VSFDMAMRQLGGTTIVMDSGSMQLGRGETIADTARVLSRYVDAIMIRTDDHQKAVDLAKY 121

Query: 118 ATVPVINGLTDDTHPCQLMADIMTFEEHRGPVAGKTIAWTGDGNNVLHSLLEASARFRFN 177
           A VPVINGLTD +HPCQ+MAD+ T  EH+G + G   AW GDGNNVLHS++EA +   F 
Sbjct: 122 ADVPVINGLTDRSHPCQIMADLQTILEHKGRIEGLGWAWLGDGNNVLHSIVEAGSLLGFP 181

Query: 178 LNVAVPEGSEPAQKHIDWSKAHGGKLHFTRSPEEAVDQADCVVTDCWVSMGQEHRARGHN 237
           + +  PEG +P    +  ++A GG +  +R P E V  AD VVTD W+SMGQ H      
Sbjct: 182 VRIGCPEGYDPDAGILAEARARGGGILLSRDPVEVVRGADVVVTDTWISMGQAHAEEKLA 241

Query: 238 VFSPYQVNAKLMAHAKPDALFMHCLPAHRGEEVTDEVIDGPHSVVFDEAENRLHAQKAVL 297
              P+QV+   MA A PDA F+HCLPAHRGEEV D VIDGP S+++DEAENRLHAQKAVL
Sbjct: 242 AMMPFQVDEARMAQAAPDAAFLHCLPAHRGEEVVDAVIDGPRSLIWDEAENRLHAQKAVL 301

Query: 298 AWCLG 302
            WCLG
Sbjct: 302 LWCLG 306


Lambda     K      H
   0.320    0.133    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 331
Number of extensions: 15
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 303
Length of database: 309
Length adjustment: 27
Effective length of query: 276
Effective length of database: 282
Effective search space:    77832
Effective search space used:    77832
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate WP_079647873.1 B5X82_RS09400 (ornithine carbamoyltransferase)
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00658.hmm
# target sequence database:        /tmp/gapView.3270430.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.4e-113  365.1   0.0   1.5e-113  364.9   0.0    1.0  1  NCBI__GCF_900167915.1:WP_079647873.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_900167915.1:WP_079647873.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  364.9   0.0  1.5e-113  1.5e-113       1     303 [.       3     305 ..       3     306 .. 0.95

  Alignments for each domain:
  == domain 1  score: 364.9 bits;  conditional E-value: 1.5e-113
                             TIGR00658   1 rhllslldlseeelkellelakklkkekkkgke.....ekklkgktlaliFekrstRtRvsfevaayelGaqv 68 
                                           r++l+l+d   + ++ +l+ a   kk++    +     ++ l g+tla+iFek+stRtRvsf++a+ +lG+ +
  NCBI__GCF_900167915.1:WP_079647873.1   3 RSFLDLADAGAAGVRLMLDEAVARKKARAGLPKgaadrDAPLAGHTLAMIFEKNSTRTRVSFDMAMRQLGGTT 75 
                                           6899****************999998875422234444899******************************** PP

                             TIGR00658  69 lylnkeelqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDlltike 141
                                           +++++ ++qlgr+e+i+Dtarvlsryvdai++R+ +h++  +lakya vPvingLtd +hPcqi+aDl+ti e
  NCBI__GCF_900167915.1:WP_079647873.1  76 IVMDSGSMQLGRGETIADTARVLSRYVDAIMIRTDDHQKAVDLAKYADVPVINGLTDRSHPCQIMADLQTILE 148
                                           ************************************************************************* PP

                             TIGR00658 142 klgklkevklvyvGDannvanslllaaaklGldvvvatPeglepeaeivkkakkiakenggkleltedpkkav 214
                                           ++g+++++  +++GD+nnv +s++ a+ +lG+ v++ +Peg++p+a i+ +a++     gg + l +dp++ v
  NCBI__GCF_900167915.1:WP_079647873.1 149 HKGRIEGLGWAWLGDGNNVLHSIVEAGSLLGFPVRIGCPEGYDPDAGILAEARA----RGGGILLSRDPVEVV 217
                                           ***************************************************965....9************** PP

                             TIGR00658 215 kdadviytDvwvsmGeeekkeerlkllkpyqvneellelakpevkflhCLPavrGeevtdevlegeasivfde 287
                                           ++adv++tD+w+smG+ ++ ee+l+++ p+qv+e  ++ a p++ flhCLPa+rGeev d v++g++s+++de
  NCBI__GCF_900167915.1:WP_079647873.1 218 RGADVVVTDTWISMGQAHA-EEKLAAMMPFQVDEARMAQAAPDAAFLHCLPAHRGEEVVDAVIDGPRSLIWDE 289
                                           ***************9765.59*************************************************** PP

                             TIGR00658 288 aenRlhaqkavlkall 303
                                           aenRlhaqkavl ++l
  NCBI__GCF_900167915.1:WP_079647873.1 290 AENRLHAQKAVLLWCL 305
                                           ************9877 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (309 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 24.82
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources