Alignments for a candidate for etoh-dh-nad in Sphingomonas histidinilytica UM2

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-nad in Sphingomonas histidinilytica UM2

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate WP_079650953.1 B5X82_RS24480 choline dehydrogenase

Query= BRENDA::Q76HN6
         (526 letters)



>NCBI__GCF_900167915.1:WP_079650953.1
          Length = 537

 Score =  429 bits (1103), Expect = e-124
 Identities = 236/526 (44%), Positives = 315/526 (59%), Gaps = 8/526 (1%)

Query: 4   DYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHVNWAFK 63
           DY+IVGAGSAGC+LANRLSADPS  V LLEAG  DR      P G   ++ T   +W ++
Sbjct: 3   DYIIVGAGSAGCILANRLSADPSAQVTLLEAGGSDRHIFYRMPAGFLGLMKTGMGDWGYE 62

Query: 64  TTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDVLPYFR 123
             PQPGL GR  + PRGKVLGGSSSING + +RG+  DF+ W   G  GW ++D LPYFR
Sbjct: 63  CVPQPGLNGRTMHFPRGKVLGGSSSINGQVIVRGNAGDFDGWAQSGATGWSYEDCLPYFR 122

Query: 124 KSEMHHGGSSEYHGGDGELYVS---PANRHA-ASEAFVESALRAGHSYNPDFNGATQEGA 179
           K E H GG ++  GG G + ++   PA +     +A+ ++A +AG+  NPD NG+TQEG 
Sbjct: 123 KFERHPGGDTDLRGGSGPIGITVAPPAEQMTPMGQAWFKAAEQAGYPINPDLNGSTQEGF 182

Query: 180 GYYDVTIRDGRRWSTATAFLKPVRHRSNLTVLTHTHVESIVLLGKQATGVQALIKGSRVH 239
           G  D      RR ST+  +L     R NL V++      I+    +A GV+    G R  
Sbjct: 183 GRADANFSGTRRQSTSATYLAEAIGRPNLRVISQAQATRILFRNSRAIGVEYASNGKRRT 242

Query: 240 LRARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDH-ADVVLCY 298
           +    EVIL+ G   SP LL LSG+G A  L   GIA   EL GVG+NLQDH   +V   
Sbjct: 243 IETDGEVILAGGTVNSPQLLQLSGVGPAELLRRHGIAVVQELAGVGENLQDHICQMVKQE 302

Query: 299 KSNDTSLLGFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDPGLERPDIQLHSV 358
            +   S L ++    +K   ++  YA  ++GP  SN  E  AF+KT  GLE PDIQ H +
Sbjct: 303 MTKPYSALAYTRP--LKAAISLAQYALFKSGPTLSNGLEVLAFVKTRAGLEYPDIQYHFL 360

Query: 359 IGTVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHDDDVATLL 418
               +DH RK+    GF     V RP+S G+V +AS DP +AP IDP + +  +D+ T  
Sbjct: 361 NLLYEDHGRKIIQREGFMASANVSRPQSRGNVVIASSDPLQAPLIDPRYFSDPEDMRTAR 420

Query: 419 KGYRITRDIIAQTPMASF-GLRDMYSAGLHNDEQLIELLRKRTDTIYHPIGTCKMGQDEM 477
              RI R++IAQ     F G+       + +D  L + +R   ++IYHP+GTC++G D M
Sbjct: 421 ASLRIARELIAQPAFDEFRGVEYAPGTNVQSDADLDDYIRSTANSIYHPVGTCRIGTDPM 480

Query: 478 AVVDSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWI 523
           AVVD QLRVHGI+ LR+VDAS+MPT+V GNTNAA +MIAE+A++ I
Sbjct: 481 AVVDPQLRVHGIDNLRIVDASVMPTIVSGNTNAAVMMIAEKASDII 526


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 809
Number of extensions: 34
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 537
Length adjustment: 35
Effective length of query: 491
Effective length of database: 502
Effective search space:   246482
Effective search space used:   246482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources