GapMind for catabolism of small carbon sources

 

Protein WP_101589300.1 in Brevibacterium jeotgali SJ5-8

Annotation: NCBI__GCF_900169175.1:WP_101589300.1

Length: 600 amino acids

Source: GCF_900169175.1 in NCBI

Candidate for 9 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism pccA med Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 (characterized) 44% 100% 471.5 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
propionate catabolism pccA med Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 (characterized) 44% 100% 471.5 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
L-threonine catabolism pccA med Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 (characterized) 44% 100% 471.5 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
L-valine catabolism pccA med Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 (characterized) 44% 100% 471.5 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
L-leucine catabolism liuB med Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized) 41% 82% 401.4 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
L-isoleucine catabolism pccA1 med acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized) 40% 88% 344 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
propionate catabolism pccA1 med acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized) 40% 88% 344 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
L-threonine catabolism pccA1 med acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized) 40% 88% 344 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2
L-valine catabolism pccA1 med acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized) 40% 88% 344 acetyl CoA carboxylase monomer (EC 6.4.1.2) 51% 566.2

Sequence Analysis Tools

View WP_101589300.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MTAAPAASQPDLIAADPRPVRRVLIANRGEIALRIVRACRDLGIASVAAYTAADADALFV
SLADDAYRLDGSGAAETYLSIPTMVELAHRAGADAVHPGYGYLAESGEFATAVAEAGLTW
VGPTASAIDALGDKAGARAVAQAVGAPVPQGSAGPVESIPEALDVAEEIGYPVAIKAVHG
GGGRGFRTAADAQALPAAFEAASREAKAAFGRGDCLIEQQIIRPRHVETQCLADAHGRVL
VVSTRDCTMQRRNQKVLEEAPAPGLTDEQEHLLEDASVNILRHVGYVGAATCEFLVGADG
RITFMEANARIQVEHTITEEVAGVDLVAWQLRIAAGAALPESFPAPRGHAFQFRINAEDP
THGFVPVCGTITRYREPSGPGVRMDSGIATGTVVGTDFDPMLAKLIVWGPDRESALARSR
RAIDEFALEGLPTLLPLHRSLTDSAEFAGPELRVSTRWLEEDFMPAWSAERLPQPSGDSE
APAEQVDVVVEVDGHRLVVGVPASLAAPSARPSSRSRRVTRRGTKAAAASGTLTAPMQGT
IVAVDVSPGDQVTAGDRLAVIEAMKMEQPLTAAHDAVVRSVVVGPGAGVRSGDVLVEFEA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory