GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hpcD in Brevibacterium jeotgali SJ5-8

Align 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized)
to candidate WP_101588405.1 BJEO58_RS05430 crotonase/enoyl-CoA hydratase family protein

Query= BRENDA::A4YI89
         (259 letters)



>NCBI__GCF_900169175.1:WP_101588405.1
          Length = 302

 Score =  155 bits (393), Expect = 7e-43
 Identities = 94/264 (35%), Positives = 148/264 (56%), Gaps = 8/264 (3%)

Query: 4   ETIETKKEGNLFWITLNRPDKLNALNAK-LLEELDRAVSQAESDPEIRVIIITGKGKAFC 62
           + +  +++G++   TLNRPD  N ++   ++  +  AV  A +DP +RV+I+TG GKAF 
Sbjct: 39  DALLVERDGHVETWTLNRPDSRNPISDDDMVAAIIDAVDAANADPAVRVVILTGAGKAFS 98

Query: 63  AGADITQFNQLT------PAEAWKFSKKG-REIMDKIEALSKPTIAMINGYALGGGLELA 115
           AG D+ +    +      P +     + G ++I   ++ L  P IA +NG A+G G +L 
Sbjct: 99  AGGDVKKMRDRSGMFGGHPHQMRGGYRYGIQQIPLALQRLDAPLIAAVNGPAVGAGCDLT 158

Query: 116 LACDIRIAAEEAQLGLPEINLGIYPGYGGTQRLTRVIGKGRALEMMMTGDRIPGKDAEKY 175
              D+RIA+E A      + LGI PG GG   L R++G  RA EM +TGDR+  K A ++
Sbjct: 159 AMADMRIASETAWFAESFVKLGIIPGDGGAWFLPRLVGPARAAEMTLTGDRVDAKTALEW 218

Query: 176 GLVNRVVPLANLEQETRKLAEKIAKKSPISLALIKEVVNRGLDSPLLSGLALESVGWGVV 235
           GLV+RVV   +L  E R LA+++A   P ++ + K+++     S L S L L +    + 
Sbjct: 219 GLVSRVVAPEDLMTEARALADRVAVNPPHAVRMAKKLLKESDGSSLSSLLELSAAMQPLA 278

Query: 236 FSTEDKKEGVSAFLEKREPTFKGK 259
               D  E + AFL+KR+PTF G+
Sbjct: 279 HHAPDHAEAIDAFLDKRDPTFTGE 302


Lambda     K      H
   0.315    0.135    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 172
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 259
Length of database: 302
Length adjustment: 26
Effective length of query: 233
Effective length of database: 276
Effective search space:    64308
Effective search space used:    64308
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory