Align D-xylose 1-dehydrogenase (EC 1.1.1.175) (characterized)
to candidate WP_085218047.1 B9N75_RS06390 acetoacetyl-CoA reductase
Query= BRENDA::B8H1Z0 (248 letters) >NCBI__GCF_900177405.1:WP_085218047.1 Length = 240 Score = 98.2 bits (243), Expect = 1e-25 Identities = 76/237 (32%), Positives = 110/237 (46%), Gaps = 11/237 (4%) Query: 15 VITGGGSGIGAGLTAGFARQGAEVIFLDIADEDSRALEAELAGSPIPPVYKRCDLMNLE- 73 ++TGG GIG ++ +G V +E+ E G YK D+ + E Sbjct: 6 IVTGGTRGIGEAISLALRDKGLTVAANYAGNEEKAKAFTEKTGIK---AYK-WDVADFEG 61 Query: 74 ---AIKAVFAEIGDVDVLVNNAGNDDRHKLADVTGAYWDERINVNLRHMLFCTQAVAPGM 130 A+K + A++G VDVLVNNAG + + WD+ I+VNL +AV GM Sbjct: 62 CVAAVKQIEADLGAVDVLVNNAGITRDGTMKRMDRNAWDDVIDVNLGGCFNMAKAVWEGM 121 Query: 131 KKRGGGAVINFGSISWHLGLEDLVLYETAKAGIEGMTRALARELGPDDIRVTCVVPGNVK 190 +R G ++N GSI+ G V Y AK+GI G T+ALA+E I V + PG + Sbjct: 122 SERKYGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKALAQEGARAGITVNAIAPGYID 181 Query: 191 TKRQEKWYTPEGEAQIVAAQCLKGRI-VPENVAALVLFLASDDASLCTGHEYWIDAG 246 T P Q + A+ GR+ +A V FL ++D TG I+ G Sbjct: 182 TDMVAA--VPADVLQKIVAKIPVGRLGQASEIARGVTFLCAEDGGFITGSTLSINGG 236 Lambda K H 0.319 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 155 Number of extensions: 13 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 248 Length of database: 240 Length adjustment: 24 Effective length of query: 224 Effective length of database: 216 Effective search space: 48384 Effective search space used: 48384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory