GapMind for catabolism of small carbon sources

 

Protein WP_085544283.1 in Dethiosulfovibrio salsuginis USBA 82

Annotation: NCBI__GCF_900177735.1:WP_085544283.1

Length: 446 amino acids

Source: GCF_900177735.1 in NCBI

Candidate for 3 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-tryptophan catabolism tnaT lo High affinity tryptophan:Na+ symporter, TnaT, of 501 aas and 12 TMSs (Androutsellis-Theotokis et al., 2003). The Km for Tryptophan is 145 nM; tryptamine and serotonin weakly inhibited with Ki values of 200 and 440 μM, respectively. An evolutionarily conserved role of adjacent transmembrane segments 7 and 8 has been proposed (characterized) 32% 88% 235.7 Neurotransmitter:sodium symporter of 455 aas, MhsT 44% 382.1
L-tyrosine catabolism tyt1 lo The 11 TMS Na+-dependent tyrosine transporter, Tyt1 (characterized) 31% 98% 215.3 Neurotransmitter:sodium symporter of 455 aas, MhsT 44% 382.1
L-alanine catabolism metP lo Transporter, component of The methionine/alanine uptake porter, MetPS (Trotschel et al., 2008) (MetP is the transporter; MetS is an essential auxiliary subunit) (characterized) 35% 70% 213.8 Neurotransmitter:sodium symporter of 455 aas, MhsT 44% 382.1

Sequence Analysis Tools

View WP_085544283.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKAESSRGEWSSKIGFILAAAGSAIGLGSIWRFPYITGQSGGAVFVLVYLFLVFTIGISL
MMAEIVIGKKGRLNAVGSFRRLGGKHWGLVGWIGVIAAFVILSYYGVIGGWTMAYVLKSF
TGLISTSGSGEVAKIFEAFISDGKQMLFYQGLFMAATVIVVFRGVSGGIERLCTFCMPAL
FILMILLIGRALTLPGAMEGVVFFLKPDFSKLSGEVVLSAMGQAFFSLSLGIGAMVIYGS
YLPDKTDIPKASLQICFLTFMISLMAGLIIFPSLFAFGMEPGAGAGLTFITLPVVFAKMP
GGVLWAASFFVLFFFAALTSSVSLLEVVVSYMIDSLGWDRKKATILLGVLISVVGIPSAL
SQGAVTINVFGVSFLDAADFFASNLLMPAGGFLTAVFIGWVAYSAGFDGITGGGAVPFRH
KKLWMFILRFIAPVCILVIFIAGLKW

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory