Alignments for a candidate for PS417_11890 in Dethiosulfovibrio salsuginis USBA 82

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_11890 in Dethiosulfovibrio salsuginis USBA 82

Align m-Inositol ABC transporter, ATPase component (itaA) (characterized)
to candidate WP_085544213.1 B9Y55_RS04705 ABC transporter ATP-binding protein

Query= reanno::pseudo3_N2E3:AO353_21385
         (521 letters)



>NCBI__GCF_900177735.1:WP_085544213.1
          Length = 505

 Score =  310 bits (795), Expect = 6e-89
 Identities = 180/494 (36%), Positives = 277/494 (56%), Gaps = 17/494 (3%)

Query: 28  LEIINVSKGFPGVVALSDVQLRVRPGSVLALMGENGAGKSTLMKIIAGIYQPDAGELRLR 87
           L I  ++K F   VA+ D+ + ++ G++ A++GENGAGKST+MK I GI+ PD GE+ + 
Sbjct: 4   LSIKGLTKTFGPFVAVDDISVDIKGGTIHAVVGENGAGKSTIMKCIYGIHHPDRGEISMD 63

Query: 88  GKPVTFDTPLAALQAGIAMIHQELNLMPHMSIAENIWIGREQLNGFHMIDHREMHRCTAQ 147
           GKP+   +P  A+ +GI M+HQ   L+P MS+  N+ +G E + G    D         +
Sbjct: 64  GKPLYIRSPRDAMASGIGMVHQHFMLVPSMSVCRNVVLGDEPVKGL-AFDLNRARSEVFR 122

Query: 148 LLERLRINLDPEEQVGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAITDKEVAHLFSII 207
           L+E   +++ P+  VG L +  +Q VEI K +   +++LI DEPT+ ++ KEV  LF  +
Sbjct: 123 LIELYGLDISPDVPVGTLPVGLQQQVEILKLLYRQAEVLIFDEPTAVLSPKEVGRLFETL 182

Query: 208 ADLKAQGKGIIYITHKMNEVFSIADEVAVFRDGAYIGLQRADSMDGDSLISMMVGRELSQ 267
              K  GK +I+I H + EV  I+D ++V R G+ I  + A  +D  SL  +MVGR ++ 
Sbjct: 183 RGFKDAGKTVIFIAHNLGEVLDISDNISVMRKGSLIDTRPASELDKSSLAELMVGRAIN- 241

Query: 268 LFPVREKPIGDLLMSVRDLRLDGV---------FKGVSFDLHAGEILGIAGLMGSGRTNV 318
           L  V + P+   L     L L G+            VS  +H GE+LG+AG+ G+G++ +
Sbjct: 242 LPSVTDGPV---LSKTPLLELSGISVAGDSRPLLDDVSLKIHGGEVLGVAGITGNGQSEL 298

Query: 319 AEAIFGITPSDGGEICLDGQPVRISDPHMAIEKGFALLTEDRKLSGLFPCLSVLEN--ME 376
            E I G+  SD G + +DG  +  SD H   E G A + EDR  +GL P  S+ +N  M 
Sbjct: 299 EEVISGLRESD-GTVVIDGVDLTSSDSHRRRESGLAYIPEDRLKTGLAPLASLADNGLMG 357

Query: 377 MAVLPHYAGNGFIQQKALRALCEDMCKKLRVKTPSLEQCIDTLSGGNQQKALLARWLMTN 436
                 +    F  +    +  + +  K  V          TLSGGN Q+ ++ R L  +
Sbjct: 358 YQYQDRFRNGPFQNRAESLSHIDGIMDKYGVAAAHRAVQSGTLSGGNMQRLVMGRELEHD 417

Query: 437 PRILILDEPTRGIDVGAKAEIYRLISYLASEGMAVIMISSELPEVLGMSDRVMVMHEGDL 496
           P++L++ +PTRG+D+G   EI+R I  L S G AV++ISS+L EVL +SDRV VM  G +
Sbjct: 418 PKVLVVSQPTRGVDIGGAEEIHRHILDLRSRGSAVLLISSDLEEVLSLSDRVAVMFRGKI 477

Query: 497 MGTLDRSEATQERV 510
           +  L   EAT++RV
Sbjct: 478 VALLSSKEATRDRV 491



 Score = 87.8 bits (216), Expect = 8e-22
 Identities = 63/225 (28%), Positives = 106/225 (47%), Gaps = 6/225 (2%)

Query: 295 VSFDLHAGEILGIAGLMGSGRTNVAEAIFGITPSDGGEICLDGQPVRISDPHMAIEKGFA 354
           +S D+  G I  + G  G+G++ + + I+GI   D GEI +DG+P+ I  P  A+  G  
Sbjct: 22  ISVDIKGGTIHAVVGENGAGKSTIMKCIYGIHHPDRGEISMDGKPLYIRSPRDAMASGIG 81

Query: 355 LLTEDRKLSGLFPCLSVLENMEMAVLPHYAGNGFIQQKALRALCEDMCKKLRVKTPSLEQ 414
           ++ +   L    P +SV  N+ +   P   G  F   +A   +   +  +L     S + 
Sbjct: 82  MVHQHFML---VPSMSVCRNVVLGDEP-VKGLAFDLNRARSEVFRLI--ELYGLDISPDV 135

Query: 415 CIDTLSGGNQQKALLARWLMTNPRILILDEPTRGIDVGAKAEIYRLISYLASEGMAVIMI 474
            + TL  G QQ+  + + L     +LI DEPT  +       ++  +      G  VI I
Sbjct: 136 PVGTLPVGLQQQVEILKLLYRQAEVLIFDEPTAVLSPKEVGRLFETLRGFKDAGKTVIFI 195

Query: 475 SSELPEVLGMSDRVMVMHEGDLMGTLDRSEATQERVMQLASGMSV 519
           +  L EVL +SD + VM +G L+ T   SE  +  + +L  G ++
Sbjct: 196 AHNLGEVLDISDNISVMRKGSLIDTRPASELDKSSLAELMVGRAI 240



 Score = 74.3 bits (181), Expect = 1e-17
 Identities = 58/245 (23%), Positives = 114/245 (46%), Gaps = 8/245 (3%)

Query: 27  LLEIINVSKGFPGVVALSDVQLRVRPGSVLALMGENGAGKSTLMKIIAGIYQPDAGELRL 86
           LLE+  +S        L DV L++  G VL + G  G G+S L ++I+G+ + D G + +
Sbjct: 256 LLELSGISVAGDSRPLLDDVSLKIHGGEVLGVAGITGNGQSELEEVISGLRESD-GTVVI 314

Query: 87  RGKPVTFDTPLAALQAGIAMIHQE---LNLMPHMSIAENIWIGREQLNGFHMIDHREMHR 143
            G  +T        ++G+A I ++     L P  S+A+N  +G +  + F     +    
Sbjct: 315 DGVDLTSSDSHRRRESGLAYIPEDRLKTGLAPLASLADNGLMGYQYQDRFRNGPFQNRAE 374

Query: 144 CTAQL---LERLRINLDPEE-QVGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAITDKE 199
             + +   +++  +       Q G LS    Q + + + + +D  +L++ +PT  +    
Sbjct: 375 SLSHIDGIMDKYGVAAAHRAVQSGTLSGGNMQRLVMGRELEHDPKVLVVSQPTRGVDIGG 434

Query: 200 VAHLFSIIADLKAQGKGIIYITHKMNEVFSIADEVAVFRDGAYIGLQRADSMDGDSLISM 259
              +   I DL+++G  ++ I+  + EV S++D VAV   G  + L  +     D +  +
Sbjct: 435 AEEIHRHILDLRSRGSAVLLISSDLEEVLSLSDRVAVMFRGKIVALLSSKEATRDRVGRI 494

Query: 260 MVGRE 264
           M+  E
Sbjct: 495 MLEGE 499


Lambda     K      H
   0.321    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 675
Number of extensions: 51
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 521
Length of database: 505
Length adjustment: 35
Effective length of query: 486
Effective length of database: 470
Effective search space:   228420
Effective search space used:   228420
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources