Alignments for a candidate for mglA in Dethiosulfovibrio salsuginis USBA 82

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Dethiosulfovibrio salsuginis USBA 82

Align Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate WP_085544213.1 B9Y55_RS04705 ABC transporter ATP-binding protein

Query= TCDB::G4FGN3
         (494 letters)



>NCBI__GCF_900177735.1:WP_085544213.1
          Length = 505

 Score =  315 bits (808), Expect = 2e-90
 Identities = 175/493 (35%), Positives = 288/493 (58%), Gaps = 10/493 (2%)

Query: 5   LEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEIIYE 64
           L +K + K F    A+  +S++   G +HA+VGENGAGKST+MK I G++ PD GEI  +
Sbjct: 4   LSIKGLTKTFGPFVAVDDISVDIKGGTIHAVVGENGAGKSTIMKCIYGIHHPDRGEISMD 63

Query: 65  GRGVRWNHPSEAINAGIVTVFQELSVMDNLSVAENIFMGDEEKRGIFIDYKKMYREAEKF 124
           G+ +    P +A+ +GI  V Q   ++ ++SV  N+ +GDE  +G+  D  +   E  + 
Sbjct: 64  GKPLYIRSPRDAMASGIGMVHQHFMLVPSMSVCRNVVLGDEPVKGLAFDLNRARSEVFRL 123

Query: 125 MKEEFGIEIDPEEKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETEKLFEVV 184
           + E +G++I P+  +G   + +QQ VEI + +Y++A+VLI DEPT+ L+ KE  +LFE +
Sbjct: 124 I-ELYGLDISPDVPVGTLPVGLQQQVEILKLLYRQAEVLIFDEPTAVLSPKEVGRLFETL 182

Query: 185 KSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMVGRKLEK 244
           +  K+ G  +IFI+H L E+ +I D +SV+R G  I T     L K  + E+MVGR +  
Sbjct: 183 RGFKDAGKTVIFIAHNLGEVLDISDNISVMRKGSLIDTRPASELDKSSLAELMVGRAINL 242

Query: 245 FYIKEAHEPGEV-VLEVKNLS--GER---FENVSFSLRRGEILGFAGLVGAGRTELMETI 298
             + +     +  +LE+  +S  G+     ++VS  +  GE+LG AG+ G G++EL E I
Sbjct: 243 PSVTDGPVLSKTPLLELSGISVAGDSRPLLDDVSLKIHGGEVLGVAGITGNGQSELEEVI 302

Query: 299 FGFRPKRGGEIYIEGKRVEINHPLDAIEQGIGLVPEDRKKLGLILIMSIMHN--VSLPSL 356
            G R +  G + I+G  +  +      E G+  +PEDR K GL  + S+  N  +     
Sbjct: 303 SGLR-ESDGTVVIDGVDLTSSDSHRRRESGLAYIPEDRLKTGLAPLASLADNGLMGYQYQ 361

Query: 357 DRIKKGPFISFKREKELADWAIKTFDIRPAYPDRKVLYLSGGNQQKVVLAKWLALKPKIL 416
           DR + GPF +        D  +  + +  A+   +   LSGGN Q++V+ + L   PK+L
Sbjct: 362 DRFRNGPFQNRAESLSHIDGIMDKYGVAAAHRAVQSGTLSGGNMQRLVMGRELEHDPKVL 421

Query: 417 ILDEPTRGIDVGAKAEIYRIMSQLAKEGVGVIMISSELPEVLQMSDRIAVMSFGKLAGII 476
           ++ +PTRG+D+G   EI+R +  L   G  V++ISS+L EVL +SDR+AVM  GK+  ++
Sbjct: 422 VVSQPTRGVDIGGAEEIHRHILDLRSRGSAVLLISSDLEEVLSLSDRVAVMFRGKIVALL 481

Query: 477 DAKEASQEKVMKL 489
            +KEA++++V ++
Sbjct: 482 SSKEATRDRVGRI 494



 Score = 90.9 bits (224), Expect = 9e-23
 Identities = 69/224 (30%), Positives = 107/224 (47%), Gaps = 6/224 (2%)

Query: 269 ENVSFSLRRGEILGFAGLVGAGRTELMETIFGFRPKRGGEIYIEGKRVEINHPLDAIEQG 328
           +++S  ++ G I    G  GAG++ +M+ I+G      GEI ++GK + I  P DA+  G
Sbjct: 20  DDISVDIKGGTIHAVVGENGAGKSTIMKCIYGIHHPDRGEISMDGKPLYIRSPRDAMASG 79

Query: 329 IGLVPEDRKKLGLILIMSIMHNVSLPSLDRIKKGPFISFKREKELADWAIKTFDIRPAYP 388
           IG+V    +   L+  MS+  NV L   D   KG      R +      I+ + +  + P
Sbjct: 80  IGMV---HQHFMLVPSMSVCRNVVLG--DEPVKGLAFDLNRARSEVFRLIELYGLDIS-P 133

Query: 389 DRKVLYLSGGNQQKVVLAKWLALKPKILILDEPTRGIDVGAKAEIYRIMSQLAKEGVGVI 448
           D  V  L  G QQ+V + K L  + ++LI DEPT  +       ++  +      G  VI
Sbjct: 134 DVPVGTLPVGLQQQVEILKLLYRQAEVLIFDEPTAVLSPKEVGRLFETLRGFKDAGKTVI 193

Query: 449 MISSELPEVLQMSDRIAVMSFGKLAGIIDAKEASQEKVMKLAAG 492
            I+  L EVL +SD I+VM  G L     A E  +  + +L  G
Sbjct: 194 FIAHNLGEVLDISDNISVMRKGSLIDTRPASELDKSSLAELMVG 237



 Score = 73.2 bits (178), Expect = 2e-17
 Identities = 56/244 (22%), Positives = 118/244 (48%), Gaps = 10/244 (4%)

Query: 3   PILEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEII 62
           P+LE+  I         L  VS++ + GEV  + G  G G+S L ++I+G+ + D G ++
Sbjct: 255 PLLELSGISVAGDSRPLLDDVSLKIHGGEVLGVAGITGNGQSELEEVISGLRESD-GTVV 313

Query: 63  YEGRGVRWNHPSEAINAGIVTVFQE---LSVMDNLSVAENIFMG----DEEKRGIFIDYK 115
            +G  +  +       +G+  + ++     +    S+A+N  MG    D  + G F +  
Sbjct: 314 IDGVDLTSSDSHRRRESGLAYIPEDRLKTGLAPLASLADNGLMGYQYQDRFRNGPFQNRA 373

Query: 116 KMYREAEKFMKEEFGIEIDPEE-KLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQ 174
           +     +  M +++G+       + G  S    Q + + R +    KVL++ +PT  +  
Sbjct: 374 ESLSHIDGIM-DKYGVAAAHRAVQSGTLSGGNMQRLVMGRELEHDPKVLVVSQPTRGVDI 432

Query: 175 KETEKLFEVVKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIV 234
              E++   +  L+ +G A++ IS  LEE+  + D+V+V+  G+ +   S +  T++++ 
Sbjct: 433 GGAEEIHRHILDLRSRGSAVLLISSDLEEVLSLSDRVAVMFRGKIVALLSSKEATRDRVG 492

Query: 235 EMMV 238
            +M+
Sbjct: 493 RIML 496


Lambda     K      H
   0.318    0.138    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 642
Number of extensions: 41
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 494
Length of database: 505
Length adjustment: 34
Effective length of query: 460
Effective length of database: 471
Effective search space:   216660
Effective search space used:   216660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources