Potential Gaps in catabolism of small carbon sources in Sphingopyxis indica DS15
Found 90 low-confidence and 53 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 2-oxoglutarate | kgtP: 2-oxoglutarate:H+ symporter KgtP | CHB69_RS01450 | CHB69_RS00295 |
| 4-hydroxybenzoate | adh: acetaldehyde dehydrogenase (not acylating) | CHB69_RS02540 | CHB69_RS13105 |
| 4-hydroxybenzoate | mhpD: 2-hydroxypentadienoate hydratase | CHB69_RS17210 | |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| 4-hydroxybenzoate | xylF: 2-hydroxymuconate semialdehyde hydrolase | CHB69_RS00015 | CHB69_RS11190 |
| alanine | snatA: L-alanine symporter SnatA | CHB69_RS13500 | |
| arabinose | araE: L-arabinose:H+ symporter | CHB69_RS13190 | CHB69_RS14085 |
| arabinose | xacC: L-arabinono-1,4-lactonase | CHB69_RS09335 | CHB69_RS14880 |
| arabinose | xacD: L-arabinonate dehydratase | CHB69_RS09330 | CHB69_RS13125 |
| arginine | astC: succinylornithine transaminase | CHB69_RS03325 | CHB69_RS01705 |
| arginine | astE: succinylglutamate desuccinylase | | |
| arginine | rocE: L-arginine permease | | |
| asparagine | ans: asparaginase | | |
| citrate | SLC13A5: citrate:Na+ symporter | CHB69_RS04700 | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | CHB69_RS14435 | CHB69_RS18860 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | | |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | | |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | | |
| citrulline | citrullinase: putative citrullinase | CHB69_RS17135 | |
| citrulline | rocD: ornithine aminotransferase | CHB69_RS03325 | CHB69_RS01655 |
| D-alanine | cycA: D-alanine:H+ symporter CycA | CHB69_RS13745 | |
| D-alanine | dadA: D-alanine dehydrogenase | CHB69_RS08755 | |
| D-lactate | D-LDH: D-lactate dehydrogenase | CHB69_RS04300 | CHB69_RS02565 |
| D-lactate | lctP: D-lactate:H+ symporter LctP or LidP | | |
| D-serine | cycA: D-serine:H+ symporter CycA | | |
| D-serine | dsdA: D-serine ammonia-lyase | CHB69_RS12555 | CHB69_RS17055 |
| deoxyinosine | adh: acetaldehyde dehydrogenase (not acylating) | CHB69_RS02540 | CHB69_RS13105 |
| deoxyinosine | deoB: phosphopentomutase | CHB69_RS03145 | |
| deoxyinosine | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyinosine | deoD: deoxyinosine phosphorylase | | |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | CHB69_RS13110 | CHB69_RS04385 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | garK: glycerate 2-kinase | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | adh: acetaldehyde dehydrogenase (not acylating) | CHB69_RS02540 | CHB69_RS13105 |
| deoxyribose | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyribose | deoK: deoxyribokinase | | |
| deoxyribose | deoP: deoxyribose transporter | | |
| ethanol | adh: acetaldehyde dehydrogenase (not acylating) | CHB69_RS02540 | CHB69_RS13105 |
| fucose | aldA: lactaldehyde dehydrogenase | CHB69_RS04470 | CHB69_RS13105 |
| fucose | fucA: L-fuculose-phosphate aldolase FucA | | |
| fucose | fucI: L-fucose isomerase FucI | | |
| fucose | fucK: L-fuculose kinase FucK | | |
| fucose | fucP: L-fucose:H+ symporter FucP | CHB69_RS19545 | CHB69_RS00850 |
| fucose | fucU: L-fucose mutarotase FucU | | |
| galactose | dgoD: D-galactonate dehydratase | CHB69_RS09330 | CHB69_RS13125 |
| galactose | dgoK: 2-dehydro-3-deoxygalactonokinase | CHB69_RS12960 | CHB69_RS13285 |
| galactose | galactonolactonase: galactonolactonase (either 1,4- or 1,5-lactone) | CHB69_RS09335 | CHB69_RS14880 |
| galacturonate | kdgK: 2-keto-3-deoxygluconate kinase | CHB69_RS12960 | |
| galacturonate | uxaB: tagaturonate reductase | CHB69_RS13020 | |
| galacturonate | uxaC: D-galacturonate isomerase | CHB69_RS13015 | |
| gluconate | gntK: D-gluconate kinase | | |
| gluconate | gntT: gluconate:H+ symporter GntT | | |
| glucosamine | gamP: glucosamine PTS system, EII-CBA components (GamP/NagE) | CHB69_RS04940 | |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | CHB69_RS13150 | CHB69_RS13010 |
| glucuronate | kdgK: 2-keto-3-deoxygluconate kinase | CHB69_RS12960 | |
| glucuronate | uxaC: D-glucuronate isomerase | CHB69_RS13015 | |
| glutamate | gltP: L-glutamate:cation symporter GltP/GltT | CHB69_RS05615 | CHB69_RS00430 |
| glycerol | glpF: glycerol facilitator glpF | CHB69_RS07845 | |
| histidine | Ga0059261_1577: L-histidine transporter | CHB69_RS13745 | |
| isoleucine | acdH: (2S)-2-methylbutanoyl-CoA dehydrogenase | CHB69_RS13685 | CHB69_RS03045 |
| isoleucine | Bap2: L-isoleucine permease Bap2 | | |
| isoleucine | pccA: propionyl-CoA carboxylase, alpha subunit | CHB69_RS03015 | CHB69_RS00455 |
| L-lactate | lctO: L-lactate oxidase or 2-monooxygenase | CHB69_RS12535 | |
| L-lactate | lctP: L-lactate:H+ symporter LctP or LidP | | |
| lactose | dgoD: D-galactonate dehydratase | CHB69_RS09330 | CHB69_RS13125 |
| lactose | dgoK: 2-dehydro-3-deoxygalactonokinase | CHB69_RS12960 | CHB69_RS13285 |
| lactose | galactonolactonase: galactonolactonase (either 1,4- or 1,5-lactone) | CHB69_RS09335 | CHB69_RS14880 |
| lactose | lacP: lactose permease LacP | | |
| leucine | leuT: L-leucine:Na+ symporter LeuT | | |
| leucine | liuC: 3-methylglutaconyl-CoA hydratase | CHB69_RS13675 | CHB69_RS12770 |
| leucine | liuE: hydroxymethylglutaryl-CoA lyase | CHB69_RS01000 | |
| lysine | davA: 5-aminovaleramidase | CHB69_RS17135 | CHB69_RS00190 |
| lysine | davB: L-lysine 2-monooxygenase | | |
| lysine | davD: glutarate semialdehyde dehydrogenase | CHB69_RS07655 | CHB69_RS06610 |
| lysine | davT: 5-aminovalerate aminotransferase | CHB69_RS03325 | CHB69_RS01475 |
| lysine | lysP: L-lysine:H+ symporter LysP | | |
| mannitol | mt2d: mannitol 2-dehydrogenase | CHB69_RS13020 | CHB69_RS12955 |
| mannitol | PLT5: polyol transporter PLT5 | | |
| mannose | gluP: mannose:Na+ symporter | CHB69_RS19545 | CHB69_RS00850 |
| mannose | man-isomerase: D-mannose isomerase | | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | | |
| myoinositol | iolM: 2-inosose 4-dehydrogenase | | |
| myoinositol | iolN: 2,4-diketo-inositol hydratase | | |
| myoinositol | iolO: 5-dehydro-L-gluconate epimerase | | |
| myoinositol | iolT: myo-inositol:H+ symporter | CHB69_RS13190 | CHB69_RS14085 |
| myoinositol | kdgK: 2-keto-3-deoxygluconate kinase | CHB69_RS12960 | |
| myoinositol | uxaE: D-tagaturonate epimerase | | |
| phenylacetate | paaA: phenylacetyl-CoA 1,2-epoxidase, subunit A | | |
| phenylacetate | paaB: phenylacetyl-CoA 1,2-epoxidase, subunit B | | |
| phenylacetate | paaC: phenylacetyl-CoA 1,2-epoxidase, subunit C | | |
| phenylacetate | paaE: phenylacetyl-CoA 1,2-epoxidase, subunit E | | |
| phenylacetate | paaF: 2,3-dehydroadipyl-CoA hydratase | CHB69_RS13675 | CHB69_RS12770 |
| phenylacetate | paaJ1: 3-oxo-5,6-dehydrosuberyl-CoA thiolase | CHB69_RS08555 | CHB69_RS16130 |
| phenylacetate | paaJ2: 3-oxoadipyl-CoA thiolase | CHB69_RS08555 | CHB69_RS16130 |
| phenylacetate | paaK: phenylacetate-CoA ligase | CHB69_RS06840 | CHB69_RS07220 |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylacetate | paaZ1: oxepin-CoA hydrolase | CHB69_RS12770 | CHB69_RS02790 |
| phenylacetate | paaZ2: 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase | | |
| phenylalanine | aroP: L-phenylalanine:H+ symporter AroP | | |
| phenylalanine | hmgA: homogentisate dioxygenase | | |
| phenylalanine | maiA: maleylacetoacetate isomerase | CHB69_RS03230 | CHB69_RS16505 |
| phenylalanine | QDPR: 6,7-dihydropteridine reductase | | |
| propionate | pccA: propionyl-CoA carboxylase, alpha subunit | CHB69_RS03015 | CHB69_RS00455 |
| propionate | putP: propionate transporter; proline:Na+ symporter | CHB69_RS03945 | |
| putrescine | gabT: gamma-aminobutyrate transaminase | CHB69_RS01705 | CHB69_RS03325 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | CHB69_RS01705 | CHB69_RS03325 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | CHB69_RS06610 | CHB69_RS13105 |
| putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
| pyruvate | SLC5A8: sodium-coupled pyruvate transporter | | |
| rhamnose | aldA: lactaldehyde dehydrogenase | CHB69_RS04470 | CHB69_RS13105 |
| rhamnose | rhaA: L-rhamnose isomerase | | |
| rhamnose | rhaB: L-rhamnulokinase | | |
| rhamnose | rhaD: rhamnulose 1-phosphate aldolase | | |
| rhamnose | rhaM: L-rhamnose mutarotase | | |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsK: ribokinase | | |
| ribose | rbsU: probable D-ribose transporter RbsU | | |
| serine | snatA: L-serine transporter | CHB69_RS13500 | |
| sorbitol | sdh: sorbitol dehydrogenase | CHB69_RS12955 | CHB69_RS13020 |
| sorbitol | SOT: sorbitol:H+ co-transporter SOT1 or SOT2 | | |
| threonine | adh: acetaldehyde dehydrogenase (not acylating) | CHB69_RS02540 | CHB69_RS13105 |
| threonine | gcvP: glycine cleavage system, P component (glycine decarboxylase) | CHB69_RS05875 | CHB69_RS05870 |
| threonine | ltaE: L-threonine aldolase | CHB69_RS04650 | CHB69_RS17180 |
| threonine | snatA: L-threonine transporter snatA | CHB69_RS13500 | |
| thymidine | adh: acetaldehyde dehydrogenase (not acylating) | CHB69_RS02540 | CHB69_RS13105 |
| thymidine | deoA: thymidine phosphorylase DeoA | | |
| thymidine | deoB: phosphopentomutase | CHB69_RS03145 | |
| thymidine | deoC: deoxyribose-5-phosphate aldolase | | |
| tryptophan | aroP: tryptophan:H+ symporter AroP | | |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | | |
| tyrosine | hmgA: homogentisate dioxygenase | | |
| tyrosine | maiA: maleylacetoacetate isomerase | CHB69_RS03230 | CHB69_RS16505 |
| valine | acdH: isobutyryl-CoA dehydrogenase | CHB69_RS13685 | CHB69_RS03045 |
| valine | Bap2: L-valine permease Bap2 | | |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | CHB69_RS13680 | CHB69_RS13675 |
| valine | pccA: propionyl-CoA carboxylase, alpha subunit | CHB69_RS03015 | CHB69_RS00455 |
| xylitol | PLT5: xylitol:H+ symporter PLT5 | | |
| xylitol | xdhA: xylitol dehydrogenase | CHB69_RS13635 | CHB69_RS12955 |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory