Align Alcohol dehydrogenase class-3; EC 1.1.1.1; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; EC 1.1.1.-; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.284 (uncharacterized)
to candidate WP_089216373.1 CHB69_RS12640 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase
Query= curated2:P72324 (376 letters) >NCBI__GCF_900188185.1:WP_089216373.1 Length = 370 Score = 581 bits (1498), Expect = e-170 Identities = 286/377 (75%), Positives = 322/377 (85%), Gaps = 8/377 (2%) Query: 1 MRTRAAVAVEAGKPLEIMEVNLEGPKAGEVMVEIKATGICHTDEFTLSGADPEGMFPAIL 60 M+TRAAVA EA KPLEI+E++LEGPKAGEV+VEI ATGICHTD +TL G D EG+FP++L Sbjct: 1 MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLGGFDSEGIFPSVL 60 Query: 61 GHEGAGVVVEVGPGVTSVKPGDHVIPLYTPECRQCPSCLSQKTNLCTAIRGTQGQGLMPD 120 GHEGAGVV EVG GVTSV PGDHVIPLYTPECRQC SCLS KTNLCTAIR TQG+GLMPD Sbjct: 61 GHEGAGVVREVGAGVTSVVPGDHVIPLYTPECRQCKSCLSGKTNLCTAIRATQGKGLMPD 120 Query: 121 GTSRFSMLDGTPILHYMGCSTFSNYTVLPEIAVAKVRPDAPFDKICYIGCGVTTGIGAVI 180 GT+RFS G PI HYMGCSTFSN+TVLPEIAVAK+R DAPF CYIGCGVTTG+GAVI Sbjct: 121 GTTRFS-YKGQPIFHYMGCSTFSNFTVLPEIAVAKIREDAPFQSSCYIGCGVTTGVGAVI 179 Query: 181 NTAKVEIGAKAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNAKKEWGERFGMTHFVNPS 240 NTAKV++G VVFGLGGIGLNVIQG +LAGA+ IIGVD+N A++EWG +FGMT F+N Sbjct: 180 NTAKVQVGDNVVVFGLGGIGLNVIQGARLAGANKIIGVDINPAREEWGRKFGMTEFLNSK 239 Query: 241 EID-GDVVAHLVNMTKTPFDQIGGADYTFDCTGNVKVMRQALEACHRGWGQSIVIGVAPA 299 + DVVA +V MT GGADYTFD TGN +VMR ALEACHRGWG SI+IGVA A Sbjct: 240 GMSREDVVAAIVRMTD------GGADYTFDATGNTEVMRTALEACHRGWGTSIIIGVAEA 293 Query: 300 GAEIQTRPFQLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKIQIDPMITHILSLEEINK 359 G EI TRPFQLVTGR W+G+AFGGA+GRTDVPKIVD YM+GKI+IDPMITH++ LE+INK Sbjct: 294 GKEIATRPFQLVTGRNWRGTAFGGAKGRTDVPKIVDMYMQGKIEIDPMITHVMGLEDINK 353 Query: 360 GFDLMHAGESIRSVVVF 376 GFDLMHAGESIRSVVV+ Sbjct: 354 GFDLMHAGESIRSVVVY 370 Lambda K H 0.320 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 566 Number of extensions: 18 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 370 Length adjustment: 30 Effective length of query: 346 Effective length of database: 340 Effective search space: 117640 Effective search space used: 117640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory