Align Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6) (characterized)
to candidate WP_089214500.1 CHB69_RS03285 glutamine--fructose-6-phosphate transaminase (isomerizing)
Query= reanno::Korea:Ga0059261_1644 (347 letters) >NCBI__GCF_900188185.1:WP_089214500.1 Length = 607 Score = 159 bits (402), Expect = 2e-43 Identities = 116/354 (32%), Positives = 173/354 (48%), Gaps = 28/354 (7%) Query: 14 MEREAAEAGAAVSRMLAANRDAIERVAAR------LRASPPAVVVTCARGSSDHAATYAK 67 M++E E V++ L++ +E+ A L +V C G+S +A AK Sbjct: 252 MQKEIFEQPTVVAQTLSSYIRPLEQTVALPQMDFDLSKIDRITIVAC--GTSFYAGMVAK 309 Query: 68 YLIETLTGVPTASAALSVASLYDAPVAPGNGLCLAISQSGKSPDLLATVEHQRKAGAFVV 127 Y E VP S D + PG GL L ISQSG++ D LA + H + AG + Sbjct: 310 YWFEQFARVPVDIDVASEFRYRDPVLQPG-GLALFISQSGETADTLAALRHCKAAGQTIA 368 Query: 128 AMVNAEDSPLAALADIVIPLKAGPERSVAATKSYICSLAAIAALVAAWA---------QD 178 +VN S +A AD+++P AGPE VA+TK++ C LA +AAL A A ++ Sbjct: 369 VVVNVPTSSMAREADLLLPTHAGPEIGVASTKAFTCQLAVLAALAAHLALKKGKLSADEE 428 Query: 179 EALETAVADLPAQLERAFALDWSAAVTA--LTGASGLFVLGRGYGYGIAQEAALKFKETC 236 + + + PA L A D A A + A + LGRG Y +A E ALK KE Sbjct: 429 REIVKHLIEAPAALNAALGHDEDIAAMAHLVAPARDVLYLGRGPDYPLALEGALKLKEIS 488 Query: 237 ALHAESFSAAEVRHGPMAIVGEAFHVLAFASSDRAGESVRETVAEFRSRGAEVLLADPA- 295 +HAE +++ E++HGP+A++ EA V+ A S E + E +RG +V+L A Sbjct: 489 YIHAEGYASGEMKHGPIALIDEAVPVIVLAPSGPLFEKTVSNMQEVMARGGKVVLISDAD 548 Query: 296 -ARQAGLPAIA------AHPAIEPILIVQSFYKMANALALARGCDPDSPPHLNK 342 +AG +A HP I P++ +A +A+A+G D D P +L K Sbjct: 549 GIAEAGEGCMATIEMPRVHPLIAPLVYAVPVQLLAYHVAVAKGTDVDQPRNLAK 602 Lambda K H 0.317 0.128 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 447 Number of extensions: 23 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 347 Length of database: 607 Length adjustment: 33 Effective length of query: 314 Effective length of database: 574 Effective search space: 180236 Effective search space used: 180236 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory