Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate WP_097029020.1 CRO07_RS00230 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha
Query= metacyc::MONOMER-13589 (666 letters) >NCBI__GCF_900207575.1:WP_097029020.1 Length = 666 Score = 1243 bits (3215), Expect = 0.0 Identities = 623/666 (93%), Positives = 648/666 (97%) Query: 1 MFKKILIANRGEIACRVIKTARKMGIQTVAVYSDADRNALHVSMADEAIHIGPPPANQSY 60 MFKKILIANRGEIACRVIKTARKMGI+TVAVYSDADRNALHVSMADEAIHIGPPPANQSY Sbjct: 1 MFKKILIANRGEIACRVIKTARKMGIKTVAVYSDADRNALHVSMADEAIHIGPPPANQSY 60 Query: 61 IVIDKIMEAIKASGAEAVHPGYGFLSERMDFAAALEAAGVVFIGPPSGAIEAMGDKITSK 120 IVIDKIMEAIK SGAEAVHPGYGFLSERMDFAAALEAAGVVFIGPPS AIEAMGDKITSK Sbjct: 61 IVIDKIMEAIKQSGAEAVHPGYGFLSERMDFAAALEAAGVVFIGPPSPAIEAMGDKITSK 120 Query: 121 KLAKEAGVSTVPGYMGLIADADEAVKISNEIGYPVMIKASAGGGGKGMRIAWSEAEVKEG 180 KLA+EAGVSTVPGYMGLIADADEAVKIS++IGYPVMIKASAGGGGKGMRIAW+ E +EG Sbjct: 121 KLAQEAGVSTVPGYMGLIADADEAVKISDQIGYPVMIKASAGGGGKGMRIAWNAEEAREG 180 Query: 181 FESSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADKHGNCVYLHERECSIQRRNQKVIE 240 F+SSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADKHGNCVYLHERECSIQRRNQKVIE Sbjct: 181 FQSSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADKHGNCVYLHERECSIQRRNQKVIE 240 Query: 241 EAPSPFLDEATRKAMGEQACALAKAVGYASAGTVEFIVDGQKNFYFLEMNTRLQVEHPVT 300 EAPSPFLDEATRKAMGEQACALAKAVGYASAGTVEFIVDGQKNFYFLEMNTRLQVEHPVT Sbjct: 241 EAPSPFLDEATRKAMGEQACALAKAVGYASAGTVEFIVDGQKNFYFLEMNTRLQVEHPVT 300 Query: 301 ELITGIDLVEQMIRVAAGEKLPFQQSDLKINGWAMESRLYAEDPYRNFLPSIGRLTRYRP 360 ELITGIDLVEQMIRVAAGEKLPFQQSDLKINGWAMESRLYAEDPYRNFLPSIGRLTRYRP Sbjct: 301 ELITGIDLVEQMIRVAAGEKLPFQQSDLKINGWAMESRLYAEDPYRNFLPSIGRLTRYRP 360 Query: 361 PVESVTPTSVVRNDTGVYEGGEISMYYDPMIAKLCTWAPTREAAIEEMRLALDTFEVEGI 420 PVE+VTPTSVVRNDTGV+EGGEISMYYDPMIAKLCTWAPTREAAIEEMR+ALDTFEVEGI Sbjct: 361 PVEAVTPTSVVRNDTGVFEGGEISMYYDPMIAKLCTWAPTREAAIEEMRIALDTFEVEGI 420 Query: 421 GHNLPFVGAVMDHPRFVKGDITTAFIAEEYPDGFQGAVLDEPTLRRVAAAAAAMNRVAEI 480 GHNLPFVGAVMDHPRFV GDITTAFIAEEYPDGFQGA LDEPTLRRVAAAAAAMNRVAEI Sbjct: 421 GHNLPFVGAVMDHPRFVSGDITTAFIAEEYPDGFQGATLDEPTLRRVAAAAAAMNRVAEI 480 Query: 481 RRTRISGTMNNHERHVGVDWVVALQGESYHVSIAADREGSTVSFSDGSSLRVTSDWTPGQ 540 RR RISGTM+NHER VG DWVV+LQGE +HV IAADREGSTV+F DGSSLR TSDWTPGQ Sbjct: 481 RRARISGTMDNHERKVGDDWVVSLQGEDFHVGIAADREGSTVTFCDGSSLRCTSDWTPGQ 540 Query: 541 PLASLMVDGRPLVMKVGKIPMGFRLRLRGADLKVNVRTPRQAELALLMPEKLPPDTSKYL 600 PLA +MVDG+PLVMKVGKIPMGFR+RLRGADLKV+VRTPRQAELA+LMPEKLPPDTS++L Sbjct: 541 PLARVMVDGKPLVMKVGKIPMGFRVRLRGADLKVHVRTPRQAELAVLMPEKLPPDTSRFL 600 Query: 601 LCPMPGLVVKINVAEGDEVQEGQALATVEAMKMENILRAERRGTVKKIAAAPGASLRVDD 660 LCPMPGLVVKINV EGDEVQEGQALATVEAMKMENILRAER+G VK+IAAA G+SLRVDD Sbjct: 601 LCPMPGLVVKINVEEGDEVQEGQALATVEAMKMENILRAERKGVVKRIAAAAGSSLRVDD 660 Query: 661 VIMEFE 666 +IMEFE Sbjct: 661 IIMEFE 666 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1446 Number of extensions: 36 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 666 Length of database: 666 Length adjustment: 38 Effective length of query: 628 Effective length of database: 628 Effective search space: 394384 Effective search space used: 394384 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory