Align acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate WP_097029448.1 CRO07_RS04350 pyruvate carboxylase
Query= metacyc::MONOMER-13597 (509 letters) >NCBI__GCF_900207575.1:WP_097029448.1 Length = 1154 Score = 349 bits (895), Expect = e-100 Identities = 188/460 (40%), Positives = 281/460 (61%), Gaps = 9/460 (1%) Query: 1 MPPFSRVLVANRGEIATRVLKAIKEMGMTAIAVYSEADKYAVHTKYADEAYYIGKAPA-L 59 M F ++L+ANRGEIA RV++A EMG +AVY+E DK ++H ADEAY IG+ + + Sbjct: 1 MAEFRKILIANRGEIAIRVMRAANEMGKKTVAVYAEEDKLSLHRFKADEAYRIGEGLSPV 60 Query: 60 DSYLNIEHIIDAAEKAHVDAIHPGYGFLSENAEFAEAVEKAGITFIGPSSEVMRKIKDKL 119 +YL+I II A+ + DAIHPGYG LSEN +F EA + AGI FIGP +E MR + DK Sbjct: 61 GAYLSIPEIIRVAKMSGADAIHPGYGLLSENPDFVEACDAAGIAFIGPRAETMRALGDKA 120 Query: 120 DGKRLANMAGVPTAPGSDGPVTSIDEALKLAEKIGYPIMVKAASGGGGVGITRVDNQDQL 179 +R+A AGVP P ++ +DE + A ++GYP+M+KA+ GGGG G+ + ++ +L Sbjct: 121 SARRVAMAAGVPVIPATEVLGDDMDEVKRQAAEVGYPLMLKASWGGGGRGMRPIPSEAEL 180 Query: 180 MDVWERNKRLAYQAFGKADLFIEKYAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQK 239 D +R A AFG + ++EK + RH+E Q++GDK GN +ER+CT+QRRNQK Sbjct: 181 ADKVREGRREAEAAFGNGEGYLEKMILRARHVEVQILGDKQGNIYHLYERDCTVQRRNQK 240 Query: 240 LIEEAPSPALKMEERESMFEPIIKFGKLINYFTLGTFETAFSDVSRDFYFLELNKRLQVE 299 ++E AP+P L ++R + E + +NY GT E S+ FYF+E+N R+QVE Sbjct: 241 VVERAPAPYLTDDQRAEVCELGRRICAHVNYECAGTVEFLMDMDSQQFYFIEVNPRVQVE 300 Query: 300 HPTTELIFRIDLVKLQIKLAAGEHLPFS-----QEDLNKRVRGTAIEYRINAEDALNNFT 354 H TE + ID+V+ QI++A G L + QED+ + G A++ R+ ED NNF Sbjct: 301 HTVTEEVTGIDIVQAQIRIAEGATLAEATGVARQEDIT--LSGHALQCRVTTEDPQNNFI 358 Query: 355 GSSGFVTYYREPTGPGVRVDSGIE-SGSYVPPYYDSLVSKLIVYGESREYAIQAGIRALA 413 G +T YR TG G+R+D G +G + YYDSL+ K+ + ++ E AI RAL Sbjct: 359 PDYGRLTAYRSATGMGIRLDGGTAYAGGVITRYYDSLLVKVTAWAQTPEKAISRMDRALR 418 Query: 414 DYKIGGIKTTIELYKWIMQDPDFQEGKFSTSYISQKTDQF 453 +++I G+ T I + +++ P F + ++T +I + F Sbjct: 419 EFRIRGVATNIAFVENLLKHPSFLDYSYTTKFIDTTPELF 458 Lambda K H 0.317 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1151 Number of extensions: 46 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 509 Length of database: 1154 Length adjustment: 40 Effective length of query: 469 Effective length of database: 1114 Effective search space: 522466 Effective search space used: 522466 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory