Align BadH (characterized)
to candidate WP_097068497.1 CRO22_RS02400 beta-ketoacyl-ACP reductase
Query= metacyc::MONOMER-893 (255 letters) >NCBI__GCF_900217815.1:WP_097068497.1 Length = 240 Score = 168 bits (426), Expect = 8e-47 Identities = 94/245 (38%), Positives = 136/245 (55%), Gaps = 10/245 (4%) Query: 6 NKTAVITGGGGGIGGATCRRFAQEGAKIAVFDLNLDAAEKVAGAIRDAGGTAEAVRCDIA 65 ++ A++TGG GIG A + G + N ++ A G + V+ +A Sbjct: 2 SRVALVTGGTRGIGAAISMALKEAGYTVVA---NYAGNDEAAREFTHVTGI-KTVKWSVA 57 Query: 66 DRTSVDAAIATTTTTLGPVDILVNNAGWDIFKPFTKTEPGEWERLIAINLTGALHMHHAV 125 D T + I +GP+D+LVNNAG PF K +W+++I NL G +M H + Sbjct: 58 DYTGCEDGIRQIEDEVGPIDVLVNNAGITRDAPFHKMSREQWDQVIGTNLNGLFNMTHPL 117 Query: 126 LPGMVERRHGRIVNIASDAARVGSSGEAVYAACKGGLVAFSKTLAREHARHGITVNVVCP 185 GM ER+ GRI+NI+S + G G+ Y+A K G + F+K LA+E AR GITVN +CP Sbjct: 118 WNGMRERKFGRIINISSINGQKGQFGQVNYSAAKAGDIGFTKALAQEGARSGITVNAICP 177 Query: 186 GPTDTALLADVTSGAANPEKLIEAFTKAIPLGRLGKPDDLAGAIAFFGSDDAGFITGQVL 245 G T ++ V PEK+ E+ IP+GRLG+PD++A + F SD AGFITG Sbjct: 178 GYIATEMVMAV------PEKVRESIIATIPVGRLGEPDEIARTVVFLASDQAGFITGATF 231 Query: 246 SVSGG 250 +V+GG Sbjct: 232 TVNGG 236 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 163 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 240 Length adjustment: 24 Effective length of query: 231 Effective length of database: 216 Effective search space: 49896 Effective search space used: 49896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory