Align Phosphate propanoyltransferase; Phosphate acyltransferase PduL; Phosphotransacylase PduL; PTAC; Propanediol utilization protein PduL; EC 2.3.1.222 (characterized)
to candidate WP_097070251.1 CRO22_RS11250 phosphate propanoyltransferase
Query= SwissProt::Q9XDN5 (210 letters) >NCBI__GCF_900217815.1:WP_097070251.1 Length = 220 Score = 175 bits (444), Expect = 5e-49 Identities = 95/179 (53%), Positives = 120/179 (67%), Gaps = 2/179 (1%) Query: 22 IPLGVSNRHIHLSAQDYERLF-PGHPISEKKALLQPGQYAAEQTVTLVGPKGQLKNVRLL 80 +P+GVSNRHIHLS D + LF PG ++ KA+ QPGQYAAE+TV L GPKG++ VR+L Sbjct: 34 VPVGVSNRHIHLSRPDMDILFGPGASLTRMKAMKQPGQYAAEETVILRGPKGEIGKVRVL 93 Query: 81 GPLRSVSQVEISRTDARTLGIAAPLRMSGNLKGTPGIRLVSPFAELELPSGVIVAQRHIH 140 GPLR +Q+E+S D LGI P+RMSG L+G+ G+ +V P + +GVIVA RHIH Sbjct: 94 GPLRKETQIEVSVADGFVLGIKPPMRMSGKLEGSAGLTVVGPKGAVVKDAGVIVALRHIH 153 Query: 141 MSPLDALILRVSHGDMVSVAIEGDDRGLIFNNVAIRVSPDMRLEMHIDTDEANAAGADN 199 M+P DA L + +GD V V + G RG I NVAIR EMHID +EANA G N Sbjct: 154 MAPADASRLGLKNGDTVDVDVTG-PRGGIMRNVAIRSDEVSATEMHIDVEEANAFGLKN 211 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 186 Number of extensions: 10 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 210 Length of database: 220 Length adjustment: 22 Effective length of query: 188 Effective length of database: 198 Effective search space: 37224 Effective search space used: 37224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory