GapMind for catabolism of small carbon sources

 

2-deoxy-D-ribose catabolism in Granulicella tundricola MP5ACTX9

Best path

deoP, deoK, deoC, adh, acs

Rules

Overview: Deoxyribose utilization in GapMind is based on MetaCyc pathways 2-deoxy-D-ribose degradation I via deoxyribose 5-phosphate aldolase (link) and pathway II via oxidation to 2-deoxy-3-dehydro-D-ribonate (link).

19 steps (13 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
deoP deoxyribose transporter ACIX9_RS13540
deoK deoxyribokinase ACIX9_RS01550
deoC deoxyribose-5-phosphate aldolase ACIX9_RS09070
adh acetaldehyde dehydrogenase (not acylating) ACIX9_RS13865 ACIX9_RS14355
acs acetyl-CoA synthetase, AMP-forming ACIX9_RS13680
Alternative steps:
aacS acetoacetyl-CoA synthetase
ackA acetate kinase ACIX9_RS08440 ACIX9_RS00745
ald-dh-CoA acetaldehyde dehydrogenase, acylating
atoA acetoacetyl-CoA transferase, A subunit ACIX9_RS16065
atoB acetyl-CoA C-acetyltransferase ACIX9_RS05110 ACIX9_RS10690
atoD acetoacetyl-CoA transferase, B subunit ACIX9_RS16060
deoxyribonate-dehyd 2-deoxy-D-ribonate 3-dehydrogenase ACIX9_RS00675 ACIX9_RS21300
deoxyribonate-transport 2-deoxy-D-ribonate transporter ACIX9_RS03750 ACIX9_RS20595
drdehyd-alpha 2-deoxy-D-ribose dehydrogenase, alpha subunit ACIX9_RS19405 ACIX9_RS07575
drdehyd-beta 2-deoxy-D-ribose dehydrogenase, beta subunit
drdehyd-cytc 2-deoxyribose-D dehydrogenase, cytochrome c component
garK glycerate 2-kinase ACIX9_RS24190
ketodeoxyribonate-cleavage 2-deoxy-3-keto-D-ribonate cleavage enzyme
pta phosphate acetyltransferase

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory