GapMind for catabolism of small carbon sources

 

2-deoxy-D-ribose catabolism in Mariniradius saccharolyticus AK6

Best path

deoP, deoK, deoC, adh, ackA, pta

Rules

Overview: Deoxyribose utilization in GapMind is based on MetaCyc pathways 2-deoxy-D-ribose degradation I via deoxyribose 5-phosphate aldolase (link) and pathway II via oxidation to 2-deoxy-3-dehydro-D-ribonate (link).

19 steps (15 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
deoP deoxyribose transporter C943_RS17020
deoK deoxyribokinase C943_RS12280
deoC deoxyribose-5-phosphate aldolase C943_RS18885
adh acetaldehyde dehydrogenase (not acylating) C943_RS19630 C943_RS05050
ackA acetate kinase C943_RS13315
pta phosphate acetyltransferase C943_RS01285
Alternative steps:
aacS acetoacetyl-CoA synthetase C943_RS17400 C943_RS10765
acs acetyl-CoA synthetase, AMP-forming C943_RS11585 C943_RS10765
ald-dh-CoA acetaldehyde dehydrogenase, acylating
atoA acetoacetyl-CoA transferase, A subunit C943_RS01820
atoB acetyl-CoA C-acetyltransferase C943_RS01680 C943_RS12510
atoD acetoacetyl-CoA transferase, B subunit C943_RS01815
deoxyribonate-dehyd 2-deoxy-D-ribonate 3-dehydrogenase C943_RS20455 C943_RS05395
deoxyribonate-transport 2-deoxy-D-ribonate transporter
drdehyd-alpha 2-deoxy-D-ribose dehydrogenase, alpha subunit C943_RS07675 C943_RS13940
drdehyd-beta 2-deoxy-D-ribose dehydrogenase, beta subunit C943_RS10335 C943_RS13945
drdehyd-cytc 2-deoxyribose-D dehydrogenase, cytochrome c component
garK glycerate 2-kinase C943_RS18155
ketodeoxyribonate-cleavage 2-deoxy-3-keto-D-ribonate cleavage enzyme

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory