GapMind for catabolism of small carbon sources

 

2-deoxy-D-ribose catabolism in Halomonas salina B6

Best path

deoP, deoK, deoC, adh, ackA, pta

Rules

Overview: Deoxyribose utilization in GapMind is based on MetaCyc pathways 2-deoxy-D-ribose degradation I via deoxyribose 5-phosphate aldolase (link) and pathway II via oxidation to 2-deoxy-3-dehydro-D-ribonate (link).

19 steps (14 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
deoP deoxyribose transporter
deoK deoxyribokinase BN1003_RS14215
deoC deoxyribose-5-phosphate aldolase BN1003_RS10565
adh acetaldehyde dehydrogenase (not acylating) BN1003_RS06365 BN1003_RS05035
ackA acetate kinase BN1003_RS10595 BN1003_RS00970
pta phosphate acetyltransferase BN1003_RS10590 BN1003_RS00965
Alternative steps:
aacS acetoacetyl-CoA synthetase BN1003_RS15960 BN1003_RS11840
acs acetyl-CoA synthetase, AMP-forming BN1003_RS09375 BN1003_RS14720
ald-dh-CoA acetaldehyde dehydrogenase, acylating
atoA acetoacetyl-CoA transferase, A subunit BN1003_RS14040 BN1003_RS11775
atoB acetyl-CoA C-acetyltransferase BN1003_RS03690 BN1003_RS11730
atoD acetoacetyl-CoA transferase, B subunit BN1003_RS14045 BN1003_RS11770
deoxyribonate-dehyd 2-deoxy-D-ribonate 3-dehydrogenase BN1003_RS13590 BN1003_RS10965
deoxyribonate-transport 2-deoxy-D-ribonate transporter
drdehyd-alpha 2-deoxy-D-ribose dehydrogenase, alpha subunit BN1003_RS05950 BN1003_RS01535
drdehyd-beta 2-deoxy-D-ribose dehydrogenase, beta subunit
drdehyd-cytc 2-deoxyribose-D dehydrogenase, cytochrome c component
garK glycerate 2-kinase BN1003_RS06125 BN1003_RS06120
ketodeoxyribonate-cleavage 2-deoxy-3-keto-D-ribonate cleavage enzyme BN1003_RS01330

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory