Definition of L-tryptophan catabolism

As rules and steps, or see full text

Rules

Overview: Tryptophan degradation in GapMind is based on MetaCyc degradation pathways I via anthranilate (link), II via pyruvate (link), or IX via 3-hydroxyanthranilate (link). Pathway XII (link) overlaps with pathway I and is also represented. The other MetaCyc pathways do not yield fixed carbon or are not reported in prokaryotes, and are not included. For example, pathway IV yields indole-3-lactate, which could potentially be oxidized to indole-3-acetate, which has a known catabolic pathway, but no prokaryotes are known to consume tryptophan this way. Pathway VIII yields tryptophol (also known as indole-3-ethanol), which could potentially be oxidized to indole-3-acetate and consumed. Pathways X and XIII yield indole-3-propionate, which may spontaneously oxidize to kynurate, but kynurate catabolism is not reported.

• all:
  • tryptophan-transport, kynA, kynB, kyn and anthranilate-degradation
  • or tryptophan-transport and tnaA
  • or tryptophan-transport, kynA, kynB, sibC, kyn and 3-hydroxyanthranilate-degradation
  • Comment: In pathway I, dioxygenase kynA opens the non-aromatic ring, to N-formyl-L-kynureine, a hydrolase yields L-kynurenine (and formate), and a hydrolase yields anthranilate and L-alanine. In pathway II, the tryptophan is hydrolyzed to indole and pyruvate, and the indole may be secreted (as in E. coli). In pathway IX, dioxygenase kynA forms N-formyl-L-kynurenine and a hydrolase forms L-kynurenine, as in pathway I; then, oxygenase sibC forms 3-hydroxy-L-kynurenine, which is hydrolyzed to L-alanine and 3-hydroxyanthranilate.
• anthranilate-degradation:
  • anthranilate-dioxygenase and catechol-degradation
  • or hpaH and 3-hydroxyanthranilate-degradation
  • Comment: In MetaCyc pathway anthranilate degradation I (link), a dioxygenase cleaves off carbon dioxide and ammonia, leaving catechol. In MetaCyc pathway anthranilate degradation IV (link), anthranilate hydroxylase/monooxygenase (hpaH) yields 3-hydroxyanthranilate. Additional pathways are not included: the fate of 2-amino-5-oxocyclohex-1-enecarboxyl-CoA is not known (link), and anthraniloyl-CoA reductase (the only anaerobic route known, link) has not been linked to sequence.
• anthranilate-dioxygenase:
  • antA, antB and antC
  • or andAa, andAb, andAc and andAd
  • Comment: There are two forms of anthranilate dioxygenase, 3-subunit antABC or 4-subunit andAabcd.
• 3-hydroxyanthranilate-degradation: nbaC, nbaD, nbaE, nbaF, nbaG and 2-hydroxypenta-2,4-dienoate-degradation
  • Comment: 3-hydroxyanthranilate degradation is part of L-tryptophan degradation pathway XII (link). Dioxygenase NbaC cleaves the aromatic ring, yielding 2-amino-3-carboxymuconate 6-semialdehyde, a decarboxylase forms (2Z,4E)-2-aminomuconate semialdehyde, a dehydrogenase forms (2Z,4E)-2-aminomuconate, a deaminase forms (3E)-2-oxo-3-hexenedioate (also known as 2-oxalocrotonate), and a decarboxylase forms (2Z)-2-hydroxypenta-2,4-dienoate (HPD).
• catechol-degradation:
  • xylE and 2-hydroxymuconate-6-semialdehyde-degradation
  • or catA, catB, catC, pcaD and 3-oxoadipate-degradation
  • Comment: In MetaCyc pathway catechol degradation to HPD I (meta-cleavage, link), dioxygenase xylE converts catechol to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate (also known as 2-hydroxymuconate 6-semialdehyde). (Catechol degradation to HPD II also involves xylE and HPD, link.) In MetaCyc pathway catechol degradation III (ortho-cleavage, link), the 1,2-dioxygenase catA forms cis,cis-muconate, a cycloisomerase forms (+)-muconolactone, an isomerase converts this to (4,5-dihydro-5-oxofuran-2-yl)-acetate (also known as 3-oxoadipate enol lactone), and a hydrolase cleaves this to 3-oxoadipate.
• 3-oxoadipate-degradation: 3-oxodipate-CoA-transferase and pcaF
  • Comment: MetaCyc pathway 3-oxoadipate degradation (link) involves activation by CoA (using succinyl-CoA) and a thiolase (succinyltransferase) reaction that splits it to acetyl-CoA and succinyl-CoA.
• 2-hydroxymuconate-6-semialdehyde-degradation:
  • praB, praC, praD and 2-hydroxypenta-2,4-dienoate-degradation
  • or xylF and 2-hydroxypenta-2,4-dienoate-degradation
  • Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (This series of steps is part of protocatechuate para-cleavage, link, or catechol degradation II, link.) Or, hydrolase xylF forms HPD and formate. (This is part of a MetaCyc pathway for catechol degradation, link.)
• 2-hydroxypenta-2,4-dienoate-degradation: mhpD, mhpE and acetaldehyde-degradation
  • Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. In MetaCyc pathway 2-hydroxypenta-2,4-dienoate degradation (link), HPD is hydrated to (S)-4-hydroxy-2-oxopentanoate and an aldolase cleaves it to pyruvate and acetaldehyde.
• 3-oxodipate-CoA-transferase:
  • pcaI and pcaJ
  • or catI and catJ
  • Comment: Two different types of 3-oxoadipate CoA-transferases (EC 2.8.3.6) are known. They are both heteromeric with each subunit containing a CoA-transferase domain
• acetaldehyde-degradation:
  • ald-dh-CoA
  • or adh and acs
  • or adh, ackA and pta
  • Comment: Acetaldehyde can be oxidized to acetyl-CoA, or oxidized to acetate and activated to acetyl-CoA by either acetyl-CoA synthetase (acs) or by acetate kinase (ackA) and phosphate acetyltransferase (pta).
• tryptophan-transport:
  • trpP, ecfA1, ecfA2 and ecfT
  • or aroP
  • or tnaB
  • or TAT
  • or tnaT
  • Comment: Transporters were identified using query: transporter:tryptophan:L-tryptophan:trp

Steps

trpP: energy-coupling factor transporter, tryptophan-specific (S) component TrpP

• Curated sequence O07515: Probable tryptophan transport protein. Tryptophan transporter TrpP (YhaG; Tryptophan ECF transporter S component)
• Comment: Four-part transporter (bioMNY-type) with two ATPase (A1/A2) components, a transmembrane (T) component, and a substrate-specific (S) component. The A1A2T components are not specific to tryptophan.
• Total: 1 characterized proteins

ecfA1: energy-coupling factor transporter, ATPase 1 (A1) component

• UniProt sequence P40735: RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA1 {ECO:0000255|HAMAP-Rule:MF_01710}; Short=ECF transporter A component EcfA {ECO:0000255|HAMAP-Rule:MF_01710}; EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710};
• Ignore hits to Q839D5 when looking for 'other' hits (Cobalt import ATP-binding protein CbiO 1, component of ATP-dependent folic acid uptake porter, FolT/EcfT/EcfA1/EcfA2)
• Ignore hits to Q035B2 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA; EC 7.-.-.-)
• Ignore hits to A2RI01 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA; EC 7.-.-.-. Energy-coupling factor transporter ATP-binding protein EcfA 1, component of The biotin uptake system, BioMNY. The 3-d structure of the EcfS subunit, BioY, at 2.1Å resolution is known (Berntsson et al., 2012). BioY and ThiT from L. lactis)
• Ignore hits to Q5M243 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.-. Energy-coupling factor transporter ATP-binding protein EcfA 2, component of Riboflavin ECF transport system, EcfAA'T/RibU)
• Ignore hits to Q03PY5 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA1, component of The folate transporter, FolT/EcfAA''T (The 3-d structure is known to 3.0Å resolution (Xu et al. 2013; 4HUQ))
• Ignore hits to G12WB-158-MONOMER when looking for 'other' hits (energy-coupling factor transporter ATP-bindind protein 1)
• Ignore hits to Q03ZL6 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.-)
• Comment: Orthologs may be annotated as ecfA1 or cbiO; these have the same function (but might not be part of a tryptophan transporting system).
• Total: 1 characterized proteins

ecfA2: energy-coupling factor transporter, ATPase 2 (A2) component

• UniProt sequence P70970: RecName: Full=Energy-coupling factor transporter ATP-binding protein EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; Short=ECF transporter A component EcfA2 {ECO:0000255|HAMAP-Rule:MF_01710}; EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710}; AltName: Full=ECF transporter A component EcfA';
• Ignore hits to Q839D4 when looking for 'other' hits (Cobalt import ATP-binding protein CbiO 2, component of ATP-dependent folic acid uptake porter, FolT/EcfT/EcfA1/EcfA2)
• Ignore hits to Q5M244 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.-. Energy-coupling factor transporter ATP-binding protein EcfA 1, component of Riboflavin ECF transport system, EcfAA'T/RibU)
• Ignore hits to A2RI02 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; ECF transporter A component EcfA'; EC 3.6.3.-)
• Ignore hits to Q035B3 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; ECF transporter A component EcfA'; EC 3.6.3.-)
• Ignore hits to Q03PY6 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA2, component of The folate transporter, FolT/EcfAA''T (The 3-d structure is known to 3.0Å resolution (Xu et al. 2013; 4HUQ))
• Ignore hits to G12WB-159-MONOMER when looking for 'other' hits (energy-coupling factor transporter ATP-bindind protein 2)
• Ignore hits to Q03ZL5 when looking for 'other' hits (Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; ECF transporter A component EcfA'; EC 3.6.3.-)
• Comment: Orthologs may be annotated as ecfA2 or cbiO2; these have the same function
• Total: 1 characterized proteins

ecfT: energy-coupling factor transporter, transmembrane (T) component

• Curated sequence P70972: Energy-coupling factor transporter transmembrane protein EcfT; ECF transporter T component EcfT
• Ignore hits to I7BV49 when looking for 'other' hits (Energy-coupling factor transporter transmembrane protein EcfT, component of ATP-dependent folic acid uptake porter, FolT/EcfT/EcfA1/EcfA2)
• Ignore hits to Q5M245 when looking for 'other' hits (Energy-coupling factor transporter transmembrane protein EcfT; ECF transporter T component EcfT. Energy-coupling factor transporter transmembrane protein EcfT, component of Riboflavin ECF transport system, EcfAA'T/RibU)
• Ignore hits to A2RI03 when looking for 'other' hits (Energy-coupling factor transporter transmembrane protein EcfT; ECF transporter T component EcfT. Energy-coupling factor transporter transmembrane protein EcfT, component of The biotin uptake system, BioMNY. The 3-d structure of the EcfS subunit, BioY, at 2.1Å resolution is known (Berntsson et al., 2012). BioY and ThiT from L. lactis)
• Ignore hits to Q035B4 when looking for 'other' hits (Energy-coupling factor transporter transmembrane protein EcfT; ECF transporter T component EcfT)
• Ignore hits to G12WB-160-MONOMER when looking for 'other' hits (energy-coupling factor transporter transmembrane protein EcfT)
• Ignore hits to Q03PY7 when looking for 'other' hits (Energy-coupling factor transporter transmembrane protein EcfT, component of The folate transporter, FolT/EcfAA''T (The 3-d structure is known to 3.0Å resolution (Xu et al. 2013; 4HUQ))
• Ignore hits to Q03ZL4 when looking for 'other' hits (Energy-coupling factor transporter transmembrane protein EcfT; ECF transporter T component EcfT)
• Comment: Ignore EcfT proteins from other bacteria
• Total: 1 characterized proteins

aroP: tryptophan:H+ symporter AroP

• Curated sequence P15993: Aromatic amino acid:H+ symporter, AroP of 457 aas and 12 TMSs (Cosgriff and Pittard 1997). Transports phenylalanine, tyrosine and tryptophan. aromatic amino acid:H⁺ symporter AroP. aromatic amino acid:H⁺ symporter AroP
• Curated sequence F2HN33: Transporter for phenylalainine, tyrosine and tryptophan of 449 aas and 12 TMSs, FywP or YsjA
• Curated sequence Q2VQZ4: Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids)
• Curated sequence Q46065: Aromatic amino acid permease, AroP
• UniProt sequence A0A0N9WG97: SubName: Full=Amino acid permease {ECO:0000313|EMBL:ALI00611.1};
• UniProt sequence Q4KIP0: SubName: Full=Aromatic amino acid transport protein AroP {ECO:0000313|EMBL:AAY96158.1};
• Ignore hits to A2RMP5 when looking for 'other' hits (Aromatic amino acid permease FywP)
• Ignore hits to AO356_18530 when looking for 'other' hits (L-tyrosine transporter)
• Comment: AO353_05930 (A0A0N9WG97) from Pseudomonas fluorescens FW300-N2E3 is related to aroP and is specifically improtant for tryptophan utilization (although more so if Trp is the nitrogen source). PfGW456L13_4291 (A0A293QSB2) from P. fluorescens GW4560-L13 is related to aroP and is specifically important for tryptophan utilization. Unfortunately A0A293QSB2 is no longer in UniProt; the closest remaining sequence is Q4KIP0 (only 87% identical). FywP (A2RMP5) may well be a tryptophan transporter as well, so ignore.
• Total: 6 characterized proteins

tnaB: tryptophan:H+ symporter TnaB

• Curated sequence CH_000783: low affinity tryptophan permease. Low affinity (70. tryptophan:H⁺ symporter TnaB. tryptophan:H⁺ symporter TnaB
• Curated sequence Q02DS7: Tryptophan-specific transport protein; Tryptophan permease
• Curated sequence P0AAD2: High affinity (3. tryptophan:H⁺ symporter Mtr. tryptophan:H⁺ symporter Mtr
• Total: 3 characterized proteins

TAT: tryptophan permease

• Curated sequence CH_091156: tryptophan permease. Tryptophan permease; Tryptophan amino acid transporter. Tryptophan permease, Tat2. Regulated via endocytosis by ATP-binding Cassette Transporters, Pdr5 (3.A.1.205.1) and Yor1 (3.A.208.3) as well as a seven-transmembrane protein, RSB1 (9.A.27.1.2)
• Curated sequence CH_091631: valine/tyrosine/tryptophan amino-acid permease. Valine/tyrosine/tryptophan amino-acid permease 1; Tyrosine and tryptophan amino acid transporter 1. Val/Tyr/Trp permease
• Total: 2 characterized proteins

tnaT: tryptophan:Na+ symporter TnaT

• Curated sequence O50649: High affinity tryptophan:Na+ symporter, TnaT, of 501 aas and 12 TMSs (Androutsellis-Theotokis et al., 2003). The Km for Tryptophan is 145 nM; tryptamine and serotonin weakly inhibited with Ki values of 200 and 440 μM, respectively. An evolutionarily conserved role of adjacent transmembrane segments 7 and 8 has been proposed
• Total: 1 characterized proteins

ald-dh-CoA: acetaldehyde dehydrogenase, acylating

• Curated proteins or TIGRFams with EC 1.2.1.10
• Ignore hits to items matching 1.1.1.1 when looking for 'other' hits
• Ignore hits to items matching 1.1.1.71 when looking for 'other' hits
• Ignore hits to items matching 1.2.1.57 when looking for 'other' hits
• Ignore hits to Q2XQZ7 when looking for 'other' hits (4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39))
• Comment: Many enzymes are multifunctional alcohol/acetaldehyde dehydrogenases, and many close homologs have just one annotation. EC 1.2.1.57 is acylating butanal dehydrogenase, which may also act on acetaldehyde. Q2XQZ7 is probably misannotated.
• Total: 2 HMMs and 20 characterized proteins

adh: acetaldehyde dehydrogenase (not acylating)

• Curated proteins or TIGRFams with EC 1.2.1.3
• Total: 70 characterized proteins

acs: acetyl-CoA synthetase, AMP-forming

• Curated proteins or TIGRFams with EC 6.2.1.1
• Total: 1 HMMs and 28 characterized proteins

ackA: acetate kinase

• Curated proteins or TIGRFams with EC 2.7.2.1
• Curated proteins or TIGRFams with EC 2.7.2.15
• Total: 1 HMMs and 25 characterized proteins

pta: phosphate acetyltransferase

• Curated proteins or TIGRFams with EC 2.3.1.8
• Ignore hits to P32796 when looking for 'other' hits (carnitine O-acetyltransferase (EC 2.3.1.7); phosphate acetyltransferase (EC 2.3.1.8). Carnitine O-acetyltransferase, mitochondrial; Carnitine acetylase; EC 2.3.1.7)
• Comment: BRENDA misannotates yeast's carnitine acetyltransferase with EC 2.3.1.8
• Total: 1 HMMs and 18 characterized proteins

pcaI: 3-oxoadipate CoA-transferase subunit A (PcaI)

• Curated sequence CH_021928: 3-oxoadipate CoA-transferase subunit A; EC 2.8.3.6. 3-oxoadipate CoA-transferase (subunit 2/2) (EC 2.8.3.6). α subunit of β-ketoadipate succinyl-CoA transferase (EC 2.8.3.6)
• Ignore hits to items matching 2.8.3.6 when looking for 'other' hits
• Ignore hits to items matching 2.8.3.5 when looking for 'other' hits
• Comment: Ignore EC 2.8.3.5, 3-oxoacid CoA-transferase, usually active on shorter substrates but who knows?
• Total: 1 characterized proteins

pcaJ: 3-oxoadipate CoA-transferase subunit B (PcaJ)

• Curated sequence P0A102: 3-oxoadipate CoA-transferase (subunit 1/2) (EC 2.8.3.6). 3-oxoadipate CoA-transferase subunit B; EC 2.8.3.6. β subunit of β-ketoadipate succinyl-CoA transferase (EC 2.8.3.6)
• Ignore hits to items matching 2.8.3.6 when looking for 'other' hits
• Ignore hits to items matching 2.8.3.5 when looking for 'other' hits
• Total: 1 characterized proteins

catI: 3-oxoadipate CoA-transferase subunit A (CatI)

• Curated sequence Q8VPF3: 3-oxoadipate CoA-transferase subunit A; 3-oxoadipate:succinyl-CoA transferase subunit A; Beta-ketoadipate:succinyl-CoA transferase subunit A; EC 2.8.3.6. 3-oxoadipate CoA-transferase (subunit 2/2) (EC 2.8.3.6)
• Curated sequence GFF1318: 3-oxoadipate CoA-transferase (EC 2.8.3.6)
• Curated sequence PfGW456L13_4592: 3-oxoadipate CoA-transferase (EC 2.8.3.6)
• Curated sequence AO353_17195: 3-oxoadipate CoA-transferase (EC 2.8.3.6)
• Ignore hits to items matching 2.8.3.6 when looking for 'other' hits
• Ignore hits to items matching 2.8.3.5 when looking for 'other' hits
• Comment: Ignore EC 2.8.3.5, 3-oxoacid CoA-transferase, usually active on shorter substrates but who knows?
• Total: 4 characterized proteins

catJ: 3-oxoadipate CoA-transferase subunit B (CatJ)

• Curated sequence Q8VPF2: 3-oxoadipate CoA-transferase subunit B; 3-oxoadipate:succinyl-CoA transferase subunit B; Beta-ketoadipate:succinyl-CoA transferase subunit B; EC 2.8.3.6. 3-oxoadipate CoA-transferase (subunit 1/2) (EC 2.8.3.6)
• Curated sequence GFF1319: 3-oxoadipate CoA-transferase (EC 2.8.3.6)
• Curated sequence PfGW456L13_4591: 3-oxoadipate CoA-transferase (EC 2.8.3.6)
• Curated sequence AO353_17200: 3-oxoadipate CoA-transferase (EC 2.8.3.6)
• Ignore hits to items matching 2.8.3.6 when looking for 'other' hits
• Ignore hits to items matching 2.8.3.5 when looking for 'other' hits
• Total: 4 characterized proteins

mhpD: 2-hydroxypentadienoate hydratase

• Curated proteins or TIGRFams with EC 4.2.1.80
• Total: 5 characterized proteins

mhpE: 4-hydroxy-2-oxovalerate aldolase

• Curated proteins or TIGRFams with EC 4.1.3.39
• Ignore hits to Q2XQZ7 when looking for 'other' hits (4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39))
• Comment: Q2XQZ7 is misannotated in BRENDA
• Total: 1 HMMs and 11 characterized proteins

praB: 2-hydroxymuconate 6-semialdehyde dehydrogenase

• Curated proteins or TIGRFams with EC 1.2.1.85
• Curated proteins or TIGRFams with EC 1.2.1.32
• Comment: EC 1.2.1.32 is 2-aminomuconate 6-semialdehyde dehydrogenase, but it is also reported to act on 2-hydroxymuconate-6-semialdehyde; many of these proteins are very similar to EC 1.2.1.85
• Total: 10 characterized proteins

praC: 2-hydroxymuconate tautomerase

• Curated proteins or TIGRFams with EC 5.3.2.6
• Total: 9 characterized proteins

praD: 2-oxohex-3-enedioate decarboxylase

• Curated proteins or TIGRFams with EC 4.1.1.77
• Total: 1 HMMs and 7 characterized proteins

xylF: 2-hydroxymuconate semialdehyde hydrolase

• Curated proteins or TIGRFams with EC 3.7.1.9
• Total: 2 characterized proteins

pcaF: succinyl-CoA:acetyl-CoA C-succinyltransferase

• Curated proteins or TIGRFams with EC 2.3.1.174
• Total: 1 HMMs and 4 characterized proteins

pcaD: 3-oxoadipate enol-lactone hydrolase

• Curated proteins or TIGRFams with EC 3.1.1.24
• Total: 1 HMMs and 4 characterized proteins

xylE: catechol 2,3-dioxygenase

• Curated proteins or TIGRFams with EC 1.13.11.2
• Ignore hits to P23103 when looking for 'other' hits (catechol 2,3-dioxygenase (EC 1.13.11.2))
• Comment: BRENDA misannotates P23103 as the P. putida xylE (that should be, P06622)
• Total: 1 HMMs and 13 characterized proteins

catA: catechol 1,2-dioxygenase

• Curated proteins or TIGRFams with EC 1.13.11.1
• Ignore hits to CH_012040 when looking for 'other' hits (catechol 1,2-dioxygenase; EC 1.13.11.1)
• Ignore hits to P83715 when looking for 'other' hits (Catechol 1,2-dioxygenase; EC 1.13.11.1)
• Comment: Ignore two protein fragments for catA
• Total: 2 HMMs and 15 characterized proteins

catB: muconate cycloisomerase

• Curated proteins or TIGRFams with EC 5.5.1.1
• Total: 6 characterized proteins

catC: muconolactone isomerase

• Curated proteins or TIGRFams with EC 5.3.3.4
• Total: 1 HMMs and 7 characterized proteins

nbaC: 3-hydroxyanthranilate 3,4-dioxygenase

• Curated proteins or TIGRFams with EC 1.13.11.6
• Total: 1 HMMs and 8 characterized proteins

nbaD: 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase

• Curated proteins or TIGRFams with EC 4.1.1.45
• Total: 5 characterized proteins

nbaE: 2-aminomuconate 6-semialdehyde dehydrogenase

• Curated proteins or TIGRFams with EC 1.2.1.32
• Total: 7 characterized proteins

nbaF: 2-aminomuconate deaminase

• Curated proteins or TIGRFams with EC 3.5.99.5
• Total: 3 characterized proteins

nbaG: 2-oxo-3-hexenedioate decarboxylase

• Curated proteins or TIGRFams with EC 4.1.1.77
• Total: 1 HMMs and 7 characterized proteins

antA: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), large subunit AntA

• Curated sequence O85673: Anthranilate 1,2-dioxygenase large subunit; EC 1.14.12.1. anthranilate 1,2-dioxygenase, large subunit; EC 1.14.12.1
• Curated sequence GFF4629: anthranilate 1,2-dioxygenase (deaminating, decarboxylating) large subunit [EC: 1.14.12.1]
• Curated sequence PfGW456L13_2740: Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Curated sequence AO353_05950: Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 4 characterized proteins

antB: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), small subunit AntB

• Curated sequence O85674: Anthranilate 1,2-dioxygenase small subunit; EC 1.14.12.1. anthranilate 1,2-dioxygenase, small subunit; EC 1.14.12.1
• Curated sequence GFF4630: Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Curated sequence PfGW456L13_2739: Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Curated sequence AO353_05955: Anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 4 characterized proteins

antC: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), electron transfer component AntC

• Curated sequence O85675: Anthranilate 1,2-dioxygenase electron transfer component; EC 1.18.1.3. anthranilate dioxygenase reductase. anthranilate dioxygenase reductase component (EC 1.14.12.1)
• Curated sequence GFF4631: electron transfer component of the anthranilate 1,2-dioxygenase system (EC 1.14.12.1)
• Curated sequence PfGW456L13_2738: electron transfer component of the anthranilate 1,2-dioxygenase system (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 3 characterized proteins

andAa: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), ferredoxin--NAD(+) reductase component AndAa

• Curated sequence Q84BZ0: Anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase component; EC 1.18.1.3. anthranilate dioxygenase reductase component (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 1 characterized proteins

andAb: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), ferredoxin subunit AndAb

• Curated sequence Q84BZ1: Anthranilate 1,2-dioxygenase ferredoxin subunit. anthranilate dioxygenase ferredoxin component (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 1 characterized proteins

andAc: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), large subunit AndAc

• Curated sequence Q84BZ3: Anthranilate 1,2-dioxygenase large subunit; EC 1.14.12.1. anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 1 characterized proteins

andAd: athranilate 1,2-dioxygenase (deaminating, decarboxylating), small subunit AndAd

• Curated sequence Q84BZ2: Anthranilate 1,2-dioxygenase small subunit; EC 1.14.12.1. anthranilate 1,2-dioxygenase (deaminating, decarboxylating) (EC 1.14.12.1)
• Ignore hits to items matching 1.14.12.1 when looking for 'other' hits
• Total: 1 characterized proteins

hpaH: anthranilate 3-monooxygenase (hydroxylase), FADH2-dependent

• Curated proteins or TIGRFams with EC 1.14.14.8
• Total: 1 characterized proteins

kynA: tryptophan 2,3-dioxygenase

• Curated proteins or TIGRFams with EC 1.13.11.11
• Curated proteins or TIGRFams with EC 1.13.11.52
• Total: 1 HMMs and 33 characterized proteins

kynB: kynurenine formamidase

• Curated proteins or TIGRFams with EC 3.5.1.9
• Ignore hits to MONOMER-19595 when looking for 'other' hits (N-formyl-β-hydroxy-L-kynurenine formamidase (EC 3.5.1.9))
• Comment: MONOMER-19595 is involved in echinomycin biosynthesis and its molecular function is controversial, see the MetaCyc entry.
• Total: 1 HMMs and 20 characterized proteins

kyn: kynureninase

• Curated proteins or TIGRFams with EC 3.7.1.3
• Comment: 3-hydroxykynurenine hydrolase and kynurenine hydrolase are described by the same EC number.
• Total: 1 HMMs and 12 characterized proteins

tnaA: tryptophanase

• Curated proteins or TIGRFams with EC 4.1.99.1
• Curated sequence MONOMER-7584: tryptophanase subunit
• Curated sequence MONOMER-7541: tryptophanase subunit
• Comment: MONOMER-7584 and -7541 carry out the same reaction but were not linked to the EC number
• Total: 1 HMMs and 9 characterized proteins

sibC: L-kynurenine 3-monooxygenase

• Curated proteins or TIGRFams with EC 1.14.13.9
• Total: 12 characterized proteins