As text, or see rules and steps
# The canonical pathway for glucosamine utilization involves # glucosamine 6-phosphate as an intermediate, as in N-acetylglucosamine utilization (metacyc:GLUAMCAT-PWY). # GapMind also includes two other pathways: an oxidative pathway via glucosaminate ammonia-lyase, and # a transmembrane transacetylase (NagX) pathway. # ABC transporters: # A 4-part system in Sinorhizobium meliloti was identified from genetic data; # expression of this system is also induced by glucosamine or galactosamine (PMC1635973). SM_b21216 ABC transporter for D-Glucosamine, ATPase component curated:reanno::Smeli:SM_b21216 SM_b21219 ABC transporter for D-Glucosamine, permease component 1 curated:reanno::Smeli:SM_b21219 SM_b21220 ABC transporter for D-Glucosamine, permease component 2 curated:reanno::Smeli:SM_b21220 SM_b21221 ABC transporter for D-Glucosamine, substrate-binding protein curated:reanno::Smeli:SM_b21221 # Transporters and PTS systems were identified using # query: transporter:glucosamine:D-glucosamine:CPD-12538:CPD-12539 glucosamine-transport: SM_b21216 SM_b21219 SM_b21220 SM_b21221 # Pseudomonas oxidize glucosamine to glucosaminate using glucose dehydrogenase (PMID:1849886), # which is a periplasmic enzyme. # So these 4-part "glucosamine" ABC transporters, # which were identified by genetic data from various Pseudomonas, # are probably glucosaminate transporters AO353_21715 glucosaminate ABC transporter, permease component 1 curated:reanno::pseudo3_N2E3:AO353_21715 curated:reanno::pseudo6_N2E2:Pf6N2E2_2052 curated:reanno::pseudo5_N2C3_1:AO356_00475 AO353_21720 glucosaminate ABC transporter, permease component 2 curated:reanno::pseudo3_N2E3:AO353_21720 curated:reanno::pseudo5_N2C3_1:AO356_00470 AO353_21725 glucosaminate ABC transporter, ATPase component curated:reanno::pseudo3_N2E3:AO353_21725 curated:reanno::pseudo5_N2C3_1:AO356_00465 curated:reanno::pseudo6_N2E2:Pf6N2E2_2050 AO353_21710 glucosaminate ABC transporter, substrate-binding component curated:reanno::pseudo3_N2E3:AO353_21710 curated:reanno::pseudo5_N2C3_1:AO356_00480 curated:reanno::pseudo6_N2E2:Pf6N2E2_2053 glucosaminate-transport: AO353_21715 AO353_21720 AO353_21725 AO353_21710 # Unified EII-CBA systems from E. coli ("nagE") or B. subtilis ("gamP"). # Ignore a close homolog of NagE from Klebsiella, annotated as a NAG PTS system, which is not well studied # and may well act on glucosamine as well. gamP glucosamine PTS system, EII-CBA components (GamP/NagE) curated:BRENDA::P09323 curated:SwissProt::P39816 ignore:BRENDA::P45604 # PTS systems (forming glucosamine-6-phosphate) glucosamine-PTS: gamP # The manXYZ system from E. coli where manX has EII-AB, manY has EII-C, and manZ has EII-D. # (manZ is listed twice with slightly different sequence lengths) manX glucosamine PTS system, EII-AB component ManX curated:CharProtDB::CH_088329 manY glucosamine PTS system, EII-C component ManY curated:CharProtDB::CH_088330 manZ glucosamine PTS system, EII-D component ManZ curated:SwissProt::P69805 curated:TCDB::P69805 glucosamine-PTS: manX manY manZ # Other transporters: SLC2A2 glucosamine transporter SLC2A2 curated:SwissProt::P11168 glucosamine-transport: SLC2A2 # periplasmic glucose dehydrogenase import glucose.steps:gdh # nagB is glucosamine 6-phosphate deaminase (isomerizing) import NAG.steps:NAG-utilization nagB # A purified glucosaminate dehydratase "alpha subunit" was determined to be # thioredoxin (SwissProt Q93HX6), which seems unlikely to physiologically relevant. glucosaminate-lyase glucosaminate ammonia-lyase EC:4.3.1.9 ignore:SwissProt::Q93HX6 # The entry for Q9ZU29 is erroneous (the correct accession is Q97U29). # EcoCyc 2-dehydro-3-deoxygalactonokinase (dgoK) is given this EC number as well but # I could not determine why. It doesn't link to the ketodeoxygluconate kinase reaction. kdgK 2-keto-3-deoxygluconate kinase EC:2.7.1.45 EC:2.7.1.178 ignore:BRENDA::Q9ZU29 ignore:ecocyc::DEHYDDEOXGALACTKIN-MONOMER kdgA 2-keto-3-deoxygluconate-6-phosphate aldolase EC:4.1.2.14 EC:4.1.2.55 # The glucosaminate pathway begins with the periplasmic glucose dehydrogenase (gdh; PMID:1849886). # (It is not clear if gdh forms glucosaminate directly or forms a lactone intermediate; # the latter seems more likely but has not been demonstrated, nor has a lactonase # been identified by genetics; or the lactone might hydrolyze # spontaneously.) # Glucosaminate is then taken up and converted to 2-keto-3-deoxygluconate by an # ammonia-lyase (EC:4.3.1.9) and phosphorylated (by kdgK) to enter the Entner-Doudoroff pathway. # (No phenotypes for kdgK were identified in the genetic data, but this step could be genetically redundant. # It is also reported that the glucosaminate dehydratase has # some aldolase activity, producing glyceraldehye and pyruvate (PMID:7766176).) all: gdh glucosaminate-transport glucosaminate-lyase kdgK kdgA # Fitness data confirms that SM_b21217 (Q92VI2), a proposed glucosamine kinase, is involved in glucosamine utilization. glc-kinase glucosamine kinase EC:2.7.1.8 EC:2.7.1.147 uniprot:Q92VI2 # Alternatively, glucosamine kinase forms glucosamine-6-phosphate, # which can be converted by nagB (glucosamine 6-phosphate deaminase (isomerizing)) # to fructose-6-phosphate. all: glucosamine-transport glc-kinase nagB # Glucosamine 6-phosphate can also be formed by PTS systems. all: glucosamine-PTS nagB # These NagX proteins are distantly related to human HGSNAT (uniprot:Q68CP4), # which is a transmembrane acetyl-CoA:alpha-glucosaminide N-acetyltransferase. # Genetic data suggests that these bacterial homologs are involved in glucosamine # utilization, but not as a transporter -- N-acetylglucosamine utilization # genes are also involved. So, they appear to be transmembrane N-acetyltransferases # for glucosamine. The key histidine which holds the acetyl group as it passes # through the membrane (His269 in NG_009552.1, or His297 # in Q68CP4) is conserved in these proteins. # The bacterial proteins with clear evidence for this role are: # Shewana3_3111 (A0KZW6), Sama_0947 (A1S448), and Echvi_1106 (L0FVP4). nagX transmembrane glucosamine N-acetyltransferase NagX uniprot:A0KZW6 uniprot:A1S448 uniprot:L0FVP4 # Alternatively, the transmembrane transacetylase (NagX) route involves # conversion in the periplasm to N-acetylglucosamine. all: nagX NAG-utilization
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory