As text, or see rules and steps
# Glucose-6-phosphate is a central metabolic intermediate, so GapMind # only represents uptake. Alternatively, glucose-6-phosphate could be # hydrolyzed before uptake by a glucose transporter. This probably # occurs in Pseudomonas simiae WCS417, but specific hydrolases for # glucose-6-phosphate have not been identified, so this pathway is not # represented in GapMind. # In more detail: mutant fitness data suggests that Pseudomonas simiae # WCS417 hydrolyzes glucose-6-phosphate to glucose before # uptake. (PS417_22685, a putative cytoplasmic glucokinase, is very # important for utilization, and the transporter # PS417_22145:PS417_22130, which is also very important, is closely # related to glucose transporters in other Pseudomonas.) But the # mutant data did not identify the putative hydrolase. It's possible # that the hydrolase is secreted and is not important for fitness in a # pooled assay. Most bacteria that can utilize glucose do not grow on # glucose-6-phosphate (PMID:29769716), so specific enzymes are # probably needed, but little is known about them. E. coli alkaline # phosphatase has the necessary activity in vitro, but the # glucose-6-phosphate transporter (uhpT) is still somewhat important # for utilization. # The homolog from Listeria is known as Hpt and is required for utilization of glucose-6-phosphate (PMID:11756655) uhpT glucose-6-phosphate:phosphate antiporter curated:ecocyc::UHPT-MONOMER curated:TCDB::Q9Z7N9 uniprot:Q8VMX0 glucose-6-P-transport: uhpT all: glucose-6-P-transport
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory